Quantitative Determination of Individual C19O2 and C19O3 Urinary 17-Ketosteroids by Gas Chromatography.

Sparagana, Mario; Keutmann, E. Henry; Mason, W. B.

doi:10.1021/ac60202a006

Cited by 21 publications

(3 citation statements)

References 21 publications

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“…Total neutral 17-oxosteroid and 17-oxogenic steroid levels were measured by standard methods (Medical Research Council Committee on Clinical Endocrinology, 1963) and previously described methods were used for oestriol (Palmer, 1964), pregnanediol (Klopper, Michie & Brown, 1955) and pregnanetriol (Morris, 1959 of Kirschner & Lipsetf (1963) with an additional partition step between benzene and 50 % methanol before thin layer chromatography (Sparagana, Keutman & Mason, 1960). Testicular volume.…”

Section: Patientsmentioning

confidence: 99%

Treatment of Eunuchoidal Men With Human Chorionic Gonadotrophin and Follicle-Stimulating Hormone

Crooke¹,

Davies²,

Morris³

1968

Journal of Endocrinology

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SUMMARY Large doses of human chorionic gonadotrophin and human pituitary follicle-stimulating hormone were given singly and in combination to six eunuchoidal men. None had an increased excretion of urinary gonadotrophin before treatment. Histological examination of the testicles showed very immature germinal epithelium in five of the patients before treatment. Spermatozoa were found histologically in three patients, only after combined treatment with both gonadotrophins. Low concentrations of spermatozoa appeared in semen from two of these patients. One patient was found to have histological evidence of spermatogenesis before treatment but was unable to produce semen. Treatment with chorionic gonadotrophin alone enabled him to produce semen containing up to 15,000,000 spermatozoa per ml. Significant increases were found in the urinary levels of a variety of steroids and in total body potassium after treatment with chorionic gonadotrophin and a variable amount of somatic development took place.

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Section: Patientsmentioning

confidence: 99%

Treatment of Eunuchoidal Men With Human Chorionic Gonadotrophin and Follicle-Stimulating Hormone

Crooke¹,

Davies²,

Morris³

1968

Journal of Endocrinology

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“…The procedure of Sparagana et al (1963) was followed to quantitate the crude glucoside mixtures. Pure samples of the a-and ß-glueosides, isolated on the AG 1-X8 column, and -D-glucose were separately mixed with known amounts of S (1,2-O-isopropylidene-a-D-glucofuranose served as the internal standard), and then each mixture was chromatographed.…”

mentioning

confidence: 99%

Allyl Glucosides-Preparation and Chromatographic Separation of Anomeric Mixture

Otey¹,

Westhoff²,

Mehltretter³

1972

Product R&D

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“…Several workers have attempted to increase the speed of analysis of urinary 11-deoxy-17-oxo steroids by the application of gas-liquid chromatography (Haahti, VandenHeuvel & Horning, 1961;Sparagana, Keutmann & Mason, 1963). Unfortunately, no stationary phase has yet been found that separates dehydroepiandrosterone, androsterone and aetiocholanolone in their free forms.…”

mentioning

confidence: 99%

The gas-liquid chromatography of carboxylic acid esters of the urinary 11-deoxy-17-oxo steroids. Determination as n-butyrates

Sadler¹,

Kellie²

1967

Biochemical Journal

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1. The gas-liquid-chromatographic separations of the acetate, propionate, n-butyrate, isobutyrate and n-valerate esters of androsterone, aetiocholanolone and dehydroepiandrosterone were studied on a 1% neopentyl glycol sebacate column. The n-butyrate, isobutyrate and n-valerate esters were well resolved. 2. The three steroids derived from hydrolysed urinary 17-oxo steroid conjugate extracts were analysed by gas-liquid chromatography after conversion into their n-butyrate esters. The results were compared with independent determinations involving chromatography on alumina.

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Quantitative Determination of Individual C₁₉O₂ and C₁₉O₃ Urinary 17-Ketosteroids by Gas Chromatography.

Cited by 21 publications

References 21 publications

Treatment of Eunuchoidal Men With Human Chorionic Gonadotrophin and Follicle-Stimulating Hormone

Treatment of Eunuchoidal Men With Human Chorionic Gonadotrophin and Follicle-Stimulating Hormone

Allyl Glucosides-Preparation and Chromatographic Separation of Anomeric Mixture

The gas-liquid chromatography of carboxylic acid esters of the urinary 11-deoxy-17-oxo steroids. Determination as n-butyrates

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