Quantitative Determination of Isotopic Abundance Ratio of <sup>13</sup>C, <sup>2</sup>H, and <sup>18</sup>O in Biofield Energy Treated Ortho and Meta Toluic Acid Isomers

Abstract

O-Toluic acid (OTA) and m-toluic acid (MTA) are two isomers of toluic acid that act as an important organic intermediates, mostly used in medicines and pesticides. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2 H/ 1 H, 13 C/ 1 2C, (PM+1)/PM and 18 O/ 16 O, (PM+2)/PM, in toluic acid isomers using gas chromatography-mass spectrometry (GC-MS). The OTA and MTA samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to biofield energy treatment. The GC-MS spectra of both the isomers showed five m/z peaks due to the molecular ion peak and fragmented peaks of toluic acid derivatives. The isotopic abundance ratio of (PM+1)/PM and (PM+2)/PM were calculated for both the isomers and found significant alteration in the treated isomers. The isotopic abundance ratio of (PM+1)/PM in treated samples of OTA was decreased and then slightly increased upto 2.37% in T2, where the (PM+2)/PM in treated OTA, significantly decreased by 55.3% in T3 sample. Similarly, in case of MTA, the isotopic abundance ratio of (PM+1)/PM in the treated sample showed a slight increase the (PM+2)/PM was decreased by 11.95% in T2 as compared to their respective control. GC-MS data suggests that the biofield energy treatment on toluic acid isomers had significantly altered the isotopic abundance of 2 H, 13 C, and 18 O in OTA and MTA as compared to the control.

LC-MS and NMR Based Structural Characterization and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Consciousness Energy Healing

“…The relative intensity of the peak in the mass spectra is directly proportional to the relative isotopic abundance of the molecule and the isotopic abundance ratio analysis was followed by the scientific literature reported [36][37][38][39] method described as below: P M stands for the relative peak intensity of the parent molecular ion [M + ] expressed in percentage. In other way, it indicates the probability to A elements having only one natural isotope in appreciable abundance (for e.g.…”

“…Consequently, the percentage change in the isotopic abundance ratio of P M+2 /P M was significantly increased by 29.18% in the Biofield Energy Treated sample compared with the control sample. Thus, 13 Scientific literature [37][38][39]45] reported that the vibrational energy is closely related with the reduced mass (µ) of the compound and the alteration of the vibrational energy can affect the several properties like physicochemical, thermal properties of the molecule. The relation between the vibrational energy and the reduced mass (µ) for a diatomic molecule is expressed as below [42,45]…”

“…+ ions were increased or decreased, thereby suggesting the change in number of neutrons in the molecule. It was then postulated to the alterations in atomic mass and atomic charge through possible mediation of neutrino oscillation [37][38][39]46]. Thus, it is assumed that The Trivedi Effect ® -Energy of Consciousness Healing Treatment might offer the required energy for the neutrino oscillations.…”

“…Biofield Energy Treatment (The Trivedi Effect ® ) has been drawn attention more in the recent times for its scientifically measurable capability to transform the characteristic properties of a wide varieties living and nonliving substances such as plants [20,21], animals [22], microbes [23,24], cancer cells [25], medium [26,27], materials [28,29], pharmaceuticals [30,31], nutraceuticals [32,33], organic compounds [34,35]. The scientific study indicated that Biofield Energy Healing Treatment (The Trivedi Effect ® ) might be an alternate method for increasing or decreasing the natural isotopic abundance ratio of the substances [36][37][38][39]. The stable isotope ratio analysis has the broad application in several scientific fields for understanding the isotope effects resulting from the difference of the isotopic composition of the molecule [40,41].…”

Evaluation of Isotopic Abundance Ratio and Structural Properties of Magnesium Gluconate After Treatment with the Energy of Consciousness Using LC-MS and NMR Spectroscopy

“…This process is known as The Trivedi Effect ® -Biofield Energy Healing Treatment [15,16] [17]. Biofield Energy Treatment (The Trivedi Effect ® ) has been extensively studied with significant outcomes in many scientific fields such as agriculture [18][19][20], biotechnology [21][22][23], materials science [24][25][26][27][28], pharmaceutical sciences [29][30][31], medical science [32,33], and microbiology [34][35][36]. Literature demonstrated that Biofield Energy Healing Treatment (The Trivedi Effect ® ) might be an alternative method for increasing or decreasing the natural isotopic abundance ratio of the substances [37,38].…”