Quantitative determination of major phlorotannins in Ecklonia stolonifera

Abstract

Ecklonia stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 x 4.6 mm i.d., 5 microm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4-9.5%), and accuracy (93.9-108.7%). The limits of detection ranged from 0.06 to 0.30 microg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 microg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia stolonifera.

Method development and validation for dieckol in the standardization of phlorotannin preparations

“…Shabir (2003) reported that R 2 > 0.999 is generally considered an acceptable value for a regression line. Because each R 2 was >0.998, the regression suggested good linearity between the peak areas and the compound concentrations over the tested range (Goo et al, 2010).…”

“…The linear regression equation was calculated from the calibration curve as Y = Ax + B, where A and B are the slope and intercept of the calibration curve, respectively, x is the concentration of dieckol, and Y is the peak area (Goo et al, 2010;Kim et al, 2013). The correlation coefficient (R 2 ) values were determined for the calibration curve.…”

“…However, a validation for the standardization of phlorotannin products has not been reported. Dieckol is generally the most abundant compound in phlorotannin preparations, and is used as an indicator compound (Shibata et al, 2004;Cho et al, 2012;Goo et al, 2010). Therefore, we developed and validated an HPLC method for the determination of dieckol for the commercialization of phlorotannin preparations.…”

Effects ofEcklonia cavaPolyphenol in Individuals with Hypercholesterolemia: A Pilot Study

“…19 E. stolonifera and E. cava are closely related brown algae species known to produce the same type of compounds, including eckol, dieckol, 6,6 0 -bieckol, 8,8 0 -bioeckol, and phlorofurofucoeckol, as major polyphenolic components. 20 Although it is not clear, several reports suggest that the anti-dyslipidemic mechanism of E. cava polyphenols may include radical scavenging activity, matrix metalloproteinase inhibitory activity, protease inhibitory activity, and antiinflammatory activity. [11][12][13][14][15][16] However, it is necessary that this uncontrolled study followed up with controlled trials to exclude the possibility of time-related bias (i.e., consequence of these lifestyle changes).…”

Quantitative Analysis of Ursolic Acid and Euscaphic Acid in Chaenomelis Fructus by HPLC-Evaporative Light Scattering Detection

“…10,11 Sometimes, the isolation of maker compounds from traditional herbal medicines is a prerequisite in quality control since most of these compounds are not commercially available. 12,13 In this study, ursolic acid and euscaphic acid ( Figure 1) were isolated from the ethanolic extract of Chaenomelis Fructus and used as marker compounds for quality control of Chaenomelis Fructus products.…”