Quantitative determination of medroxyprogesterone acetate in plasma by liquid chromatography/electrospray ion trap mass spectrometry

Abstract

A sensitive and rapid liquid chromatography/electrospray ion trap mass spectrometry (LC/MS/MS) method has been developed for the quantitative determination of medroxyprogesterone acetate (MPA) in human plasma. Plasma samples (1.0 mL) were simply extracted with pentane and the extracts were analyzed by HPLC with the detection of the analyte in the selective reaction monitoring (SRM) mode. The determination of MPA was accurate and reproducible, with a limit of quantitation of 0.05 ng/mL in plasma. The standard calibration curve for MPA was linear (r = 0.998) over the concentration range 0.05-6.0 ng/mL in human plasma. Analysis precision over the concentration range of MPA was lower than 18.8% (relative standard deviation, RSD) and accuracy was between 96.2 and 108.7%.

Medroxyprogesterone acetate impairs memory and alters the GABAergic system in aged surgically menopausal rats

“…Liquid-liquid extraction of aliquots (1 mL) from serum samples was performed according to (Kim and Kim, 2001) after adding 10 ng/mL of d 9 -progesterone (C/D/N Isotopes, Pointe-Claire, Quebec, Canada) as an internal reference compound. MPA concentrations were determined by liquid chromatography–atmospheric-pressure ionization tandem mass spectrometry.…”

Quantitative analysis of [Dmt1]DALDA in ovine plasma by capillary liquid chromatography-nanospray ion-trap mass spectrometry

“…Combined liquid chromatography and tandem mass spectrometry (LC/MS/MS) has been widely used for the quantitative analysis of trace levels of pharmaceutical drugs [5][6][7], metabolites [8,9], and peptides [10][11][12][13]. The on-line LC/MS/MS method minimizes the number of processing steps, could save analysis time, and is used for the high-throughput quantitative analysis of a large number of samples.…”

A stability-indicating HPLC method for medroxyprogesterone acetate in bulk drug and injection formulation

“…Up to now, several methods including spectrophotometry [4,5], HPLC [6][7][8][9], LC-MS [10][11][12][13][14][15][16], GC-MS [16][17][18][19][20] and immunoassay [21] have been published for determining MPA in pharmaceutical preparations and biological fluids. Two stabilityindicating HPLC methods for MPA tablet formulations have been reported.…”