Quantitative determination of mixtures of monosaccharides, disaccharides and alditols as their trimethylsilyl ether derivatives by gas-liquid chromatography

Peripheral nerve concentrations of glucose, fructose, sorbitol and myoinositol in diabetic and non-diabetic patients

“…Protein was removed by precipitation with barium hydroxide and zinc sulphate [13] and the remaining solution lyophilised. Trimethylsilyl ethers of the sugars in this sample were made using a pyridine/hexamethyldisilazine/trimethylchlorsilane mixture (10:2:1) (Pierce Chemical Company, Rockford, Ill, USA) [14]. The silylated derivatives were then partitioned between water and cyclohexane and the organic phase chromatographed using a Hewlett Packard HP 5890 GLC fitted with cross-linked methyl silicone 25-m capillary column.…”

Simultaneous quantitation of mono-, di-and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions

“…Papers [10][11][12][13][14] dealing with the separation of TMS disaccharides, with isotherm elutions, give mainly qualitative informations regarding retention indices. More successful operations can be achieved with TMS compounds of various degree of polymerisation [15][16][17][18][19][20][21][22][23][24][25]. Following pioneering works [15][16][17][18] excellent practical results have been obtained in the analysis of maltosaccharides [19][20][21], up to DP 6 [19] and DP 7 [20] or up to DP 3 including also the polyalcohol alternatives [21], as well as, in the determinations of mono-and disaccharide mixtures [22][23][24][25].…”

“…One step, simple silylation provides the less advantageous analyses: resulting, in the cases of the most common reducing saccharides, in six derivatives (the cyclic ~-and (13-pyranose/furanose anomeric pairs and the two acyclic forms, i.e., hydrate/dehydrate pairs). Thus, simple silylation allows the separation and quantitation of a limited number of saccharides only : performing the analysis of sugars both with the same [4][5][6][7][8][9][10][11][12][13][14] and with different degree of polymerisation (DP) [15][16][17][18][19][20][21][22][23][24][25]. Quantitative results have been obtained in the analysis of monosaccarides [4][5][6][7][8][9] present in ovalbumin [4], in blood [5] and in glycosaminoglycans from animal tissues [6].…”