Quantitative Determination of Monosaccharides as Their Alditol Acetates by Gas Liquid Chromatography.

Sawardeker, J. S.; Sloneker, J. H.; Jeanes, Allene

doi:10.1021/ac60231a048

Cited by 1,727 publications

(592 citation statements)

References 11 publications

Supporting

Mentioning

581

Contrasting

Unclassified

Order By: Relevance

“…Hydrolysis of polysaccharides and conversion of the resulting monosaccharides into the corresponding reduced and acetylated derivatives were done as described previously (Lindahl, 1970). Samples (1-2p1) were separated at 170°C in a Perkin-Elmer model F30 gas chromatograph, on glass columns (1 .80m x 2mm internal diameter) packed with 3 % (w/w) ECNSS-M on Gas Chrom Q (100-120mesh) (Sawardeker et al, 1965). N2 was used as carrier gas at a flow rate ofapprox.…”

Section: Methodsmentioning

confidence: 99%

Macromolecular properties and end-group analysis of heparin isolated from bovine liver capsule

Jansson¹,

Ogren²,

Lindahl³

1975

Biochemical Journal

View full text Add to dashboard Cite

Glycosaminoglycans were extracted from bovine liver capsule with 4M-guanidinium chloride, resulting in solubilization of approx. 90% of the total uronic acid-containing polysaccharide of the tissue. The extracted polysaccharide was purified and fractionated by anion-exchange chromatography on DEAE-cellulose, density-gradient ultracentrifugation in CsCl and finally gel chromatography on Sepharose 4B. By using these procedures, the two major polysaccharide components, dermatan sulphate and heparin, which constituted 55 and 30 % respectively of the total glycosaminoglycan content of the tissue, were separated from each other. Analysis of the macromolecular properties of the two polysaccharides showed that heparin existed exclusively as single polysaccharide chains, whereas dermatan sulphate occurred largely as a proteoglycan (protein content, 74% dry wt.). The purified heparin preparation was subjected to sedimentationequilibrium ultracentrifugation, indicating a molecular weight of 8800. Analysis for neutral sugars (by g.l.c.) showed 0.1 residue of xylose and 0.2 residue of galactose/ polysaccharide chain; serine amounted to 0.3 residue/polysaccharide chain. Reduction of the heparin with NaB3H4 resulted in incorporation of 3H, approximately corresponding to one reducible group/polysaccharide chain. The 3H-labelled sugar residue was liberated by a combination of acid hydrolysis and deaminative cleavage of the polysaccharide with HNO2; it was subsequently identified as an aldonic acid by paper electrophoresis. Most of the heparin chains thus contained a uronic acid residue in reducing position. It is suggested that heparin isolated from bovine liver capsule is a degradation product released from larger molecules by an endo-glycuronidase.

show abstract

Section: Methodsmentioning

confidence: 99%

Macromolecular properties and end-group analysis of heparin isolated from bovine liver capsule

Jansson¹,

Ogren²,

Lindahl³

1975

Biochemical Journal

View full text Add to dashboard Cite

show abstract

“…The hemicellulose, cellulose and lignin weight fractions present within those natural lignocellulosic materials have been also determined following a procedure described in detail elsewhere (Saeman et al, 1954;Sarwardeker et al, 1965). As shown in Fig.…”

Section: Raw Materials Compositionsmentioning

confidence: 99%

Contributions of hemicellulose, cellulose and lignin to the mass and the porous properties of chars and steam activated carbons from various lignocellulosic precursors

Cagnon¹,

Py²,

Guillot³

et al. 2009

Bioresource Technology

361

143

View full text Add to dashboard Cite

a b s t r a c tIn this study, contributions of hemicellulose, cellulose and lignin to the mass and the porous properties of chars and activated carbons from various lignocellulosic materials were studied. A predictive calculation was established using the experimental results obtained for the three components separately to evaluate the carbonization and activation yields and their respective contributions to the chars and to the subsequent activated carbons of various precursors in term of weight fraction. These equations were validated. The results showed that lignin can be considering as being the major contributor of all chars and activated carbons. Besides, the evolution of the mean pore size versus the specific porous volume showed that each component contributes to the porosity of chars and activated carbons whatever is its weight contribution.

show abstract

“…Before the gas chromatographic analysis, trifluoroacetic acid was removed from samples by freeze drying the hydrolysates in a speed-vac apparatus (Heto V-R1, Allerød, Denmark). Sugar monomers were converted to alditol acetates according to the method of Sawardeker et al (1965) and injected (25 ll) into a stainless steel column, 1AE80 m, 32 mm I.D., packed with 2% diethylene glycol adipate on 100/120 mesh Chromosorb W AW-DMCS (Supelco, Milano, Italy) fitted Table 1 Central composite design for the evaluation of the effect of pH, temperature (T,°C) and yeast extract concentration (YE, g l )1 ) on growth and exopolysaccharide (EPS) production by Streptococcus thermophilus SY in batch fermentation in a whey-based medium…”

Section: Molecular Mass Determinationmentioning

confidence: 99%

Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer

et al. 2002

View full text Add to dashboard Cite

Aims: To evaluate the effect of yeast extract (YE) concentration, temperature and pH on growth and exopolysaccharide (EPS) production in a whey-based medium by Streptococcus thermophilus SY and to characterize the partially purified EPS. Methods and Results: Factorial experiments and empirical model building were used to optimize fermentation conditions and the chemical composition, average molecular weight (MW) and rheological properties of aqueous dispersions of the EPS were determined. Exopolysaccharide production was growth associated and was higher (152 mg l -1 ) at pH 6AE4 and 36°C with 4 g l -1 YE. High performance size exclusion chromatography of the partially purified EPS showed two peaks, with a weight average MW of 2 · 10 6 and 5 · 10 4 , respectively. The EPS was a heteropolysaccharide, with a glucose : galactose : rhamnose ratio of 2 : 4AE5 : 1. Its water dispersions had a pseudoplastic behaviour and showed a higher viscosity of xanthan solutions. Significance and Impact of the Study: The fermentation conditions and some properties of an EPS produced by Strep. thermophilus, a dairy starter organism, were described.

show abstract

Quantitative Determination of Monosaccharides as Their Alditol Acetates by Gas Liquid Chromatography.

Cited by 1,727 publications

References 11 publications

Macromolecular properties and end-group analysis of heparin isolated from bovine liver capsule

Macromolecular properties and end-group analysis of heparin isolated from bovine liver capsule

Contributions of hemicellulose, cellulose and lignin to the mass and the porous properties of chars and steam activated carbons from various lignocellulosic precursors

Exopolysaccharide production by Streptococcus thermophilus SY: production and preliminary characterization of the polymer

Contact Info

Product

Resources

About