Quantitative determination of nitrogen content in plant tissue by a colorimetric method

Bilbao, Bibiana; Giraldo, Diego Fernando Barragán; Hevia, Patricio

doi:10.1080/00103629909370348

Cited by 22 publications

(8 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…e procedure described by Bilbao et al [19] was used for determination of nitrogen constituent in plant tissue. One gram of the ground sample of each treatment was weighed into crucibles and 2…”

Section: Determination Of Plant Nutrient Uptakementioning

confidence: 99%

Different Rates of Chicken Manure and NPK 15-15-15 Enhanced Performance of Sunflower (Helianthus annuusL.) on Ferruginous Soil

Abumere

Dada

Adebayo

et al. 2019

International Journal of Agronomy

View full text Add to dashboard Cite

Utilization of adequate fertilizer rate enhances soil physical and chemical properties, minimizes soil nutrient imbalance, and promotes better crop growth and development. e study investigated the influence of varying rates of chicken manure and NPK fertilizers as it affected growth, nutrient uptake, seed yield, and oil yield of sunflower on nutrient-limiting soil. Field experiments were carried out during 2014 and 2015 planting seasons for both main and residual studies.ere were eight treatments comprising four rates of chicken manure (5, 10, 15, and 20 t·ha −1 ), three rates of NPK (30, 60, and 90 kg·N·ha −1 ), and control. e experiment was laid out in a randomized complete block design with three replicates. Growth, yield, dry matter and proximate, nutrient uptake concentration, and oil content were determined following standard procedures. Data were analyzed using ANOVA, and means were compared with the Duncan multiple range test (DMRT) at p ≤ 0.05. Performance of sunflower was superior on the field fertilized with 10 t·ha −1 chicken manure which was comparable to 90 kg·N·ha −1 NPK fertilizer. Sunflower seed yield and oil quality were superior in plots supplied with 10 t·ha −1 chicken manure which was comparable to 60 kg·N·ha −1 NPK. Growth, yield, dry matter, and proximate content were least in the unfertilized plots.

show abstract

Section: Determination Of Plant Nutrient Uptakementioning

confidence: 99%

Different Rates of Chicken Manure and NPK 15-15-15 Enhanced Performance of Sunflower (Helianthus annuusL.) on Ferruginous Soil

Abumere

Dada

Adebayo

et al. 2019

International Journal of Agronomy

View full text Add to dashboard Cite

show abstract

“…Obviously, these methods are subjective, inaccurate, and unable to extend to large‐scale field monitoring, since the results depend on trained people. Some wet chemical methods, such as Kjeldahl determination, spectrophotometry, and high‐performance liquid chromatography (HPLC), are widely used to analyze the total N or photosynthetic pigment content of plants, and are proved to be accurate and repeatable . Nevertheless, these methods are destructive, time consuming, and laborious, which are not suitable for rapid online monitoring.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and quantitative diagnosis of nitrogen nutrition of tea plants under field condition using hyperspectral imaging coupled with chemometrics

Wang

Jin

et al. 2019

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: Rapid and accurate diagnosis of nitrogen (N) status in field crops is of great significance for site-specific N fertilizer management. This study aimed to evaluate the potential of hyperspectral imaging coupled with chemometrics for the qualitative and quantitative diagnosis of N status in tea plants under field conditions. RESULTS: Hyperspectral data from mature leaves of tea plants with different N application rates were preprocessed by standard normal variate (SNV). Partial least squares discriminative analysis (PLS-DA) and least squares-support vector machines (LS-SVM) were used for the classification of different N status. Furthermore, partial least squares regression (PLSR) was used for the prediction of N content. The results showed that the LS-SVM model yielded better performance with correct classification rates of 82% and 92% in prediction sets for the diagnosis of different N application rates and N status, respectively. The PLSR model for leaf N content (LNC) showed excellent performance, with correlation coefficients of 0.924, root mean square error of 0.209, and residual predictive deviation of 2.686 in the prediction set. In addition, the important wavebands of the PLSR model were interpreted based on regression coefficients. CONCLUSION: Overall, our results suggest that the hyperspectral imaging technique can be an effective and accurate tool for qualitative and quantitative diagnosis of N status in tea plants. RESULTS AND DISCUSSION Statistical analysis of LNCThe N content in the plant leaf is an indicator of soil N status. In general, the richer the N fertilizer in soil, the higher will be the total N content in plants' leaves. Here, the LNC in tea plants treated with different amount of N application was determined by the Kjeldahl method, and the results are shown in Fig. 1. It can be seen that the total N content in the leaves increased significantly with increasing J Sci Food Agric 2020; 100: 161-167

show abstract

“…Dry weight was determined after drying the plant material at 60 • C for 48 h in an oven (Heraeus Deutschland GmbH & Co. KG, Hanau, Germany). The N content per plant was analyzed using the phenol-nitroprusside method (Bilbao et al, 1999). For determining the chlorophyll and carotenoid contents, 100 mg of plant material was crushed in 10 ml of buffered acetone (acetone and sodium phosphate buffer in the ratio 4:1), centrifuged at 13,000g.…”

Section: Nitrogen Fixationmentioning

confidence: 99%

Isolation and Characterization of High-Efficiency Rhizobia From Western Kenya Nodulating With Common Bean

2021

View full text Add to dashboard Cite

Common bean is one of the primary protein sources in third-world countries. They form nodules with nitrogen-fixing rhizobia, which have to be adapted to the local soils. Commercial rhizobial strains such as Rhizobium tropici CIAT899 are often used in agriculture. However, this strain failed to significantly increase the common bean yield in many places, including Kenya, due to the local soils’ low pH. We isolated two indigenous rhizobial strains from the nodules of common bean from two fields in Western Kenya that have never been exposed to commercial inocula. We then determined their ability to fix nitrogen in common beans, solubilize phosphorus, and produce indole acetic acid. In greenhouse experiments, common bean plants inoculated with two isolates, B3 and S2 in sterile vermiculite, performed better than those inoculated with CIAT899 or plants grown with nitrogen fertilizer alone. In contrast to CIAT899, both isolates grew in the media with pH 4.8. Furthermore, isolate B3 had higher phosphate solubilization ability and produced more indole acetic acid than the other two rhizobia. Genome analyses revealed that B3 and S2 are different strains of Rhizobium phaseoli. We recommend fieldwork studies in Kenyan soils to test the efficacy of the two isolates in the natural environment in an effort to produce inoculants specific for these soils.

show abstract

Quantitative determination of nitrogen content in plant tissue by a colorimetric method

Cited by 22 publications

References 9 publications

Different Rates of Chicken Manure and NPK 15-15-15 Enhanced Performance of Sunflower (Helianthus annuusL.) on Ferruginous Soil

Different Rates of Chicken Manure and NPK 15-15-15 Enhanced Performance of Sunflower (Helianthus annuusL.) on Ferruginous Soil

Qualitative and quantitative diagnosis of nitrogen nutrition of tea plants under field condition using hyperspectral imaging coupled with chemometrics

Isolation and Characterization of High-Efficiency Rhizobia From Western Kenya Nodulating With Common Bean

Contact Info

Product

Resources

About