Quantitative determination of oxytocin receptor antagonist atosiban in rat plasma by liquid chromatography–tandem mass spectrometry

Kannan, Vivekanandan; Gadamsetty, Deepak; Rose, Madhankumar; Maria, Stella; Mustafa, Imran; Khedkar, Anand; Dave, Nitesh; Arumugam, M.A.; Iyer, Harish

doi:10.1016/j.jchromb.2010.03.011

Cited by 8 publications

(1 citation statement)

References 18 publications

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“…Tractocile® is indicated to delay imminent pre-term birth in pregnant women with regular uterine contractions of at least 30 seconds duration at a rate of 4 per 30 minutes. Literature survey [4][5][6][7][8] reveals no chromatographic methods for the estimation of Atosiban Acetate from pharmaceutical dosage forms. The availability of an HPLC method with high sensitivity and selectivity will be very useful for the determination of Atosiban Acetate in pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Estimation of Atosiban Acetate in Parenterals by Rp HPLC

Mohamud¹,

Pathi²,

Raju³

et al. 2014

J Pharm Sci Innov.

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A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Atosiban Acetate in dosage form. An XTerra MS C18 analytical column (250 x 4.6 mm, 5 μm partical size) with mobile phase consisting of mixture of buffer 0.03M Potassium Dihydrogen Orthophosphate in water and pH adjusted to 3.20 with ortho-phosphoric acid and acetonitrile in the gradient program was used. The flow rate was 1.0 mL/min and the effluents were monitored at 220 nm. The retention time was 11.3 min. The detector response was linear in the concentration of 15-225 mcg/mL. The respective linear regression equation being y = 1286.9 x-1290.8. The limit of detection (LOD) and limit of quantification (LOQ) for Atosiban Acetate were found to be 1.5 mcg/mL and 4.5 mcg/mL respectively. The percentage assay of Atosiban Acetate was 99.6 %. The method was validated by determining its accuracy, precision and linearity. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Atosiban Acetate in bulk drug and in its pharmaceutical parenterals.

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Section: Introductionmentioning

confidence: 99%

Estimation of Atosiban Acetate in Parenterals by Rp HPLC

Mohamud¹,

Pathi²,

Raju³

et al. 2014

J Pharm Sci Innov.

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show abstract

Current literature in mass spectrometry

2010

J. Mass Spectrom.

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Detection, quantification, and identification of dermorphin in equine plasma and urine by LC–MS/MS for doping control

et al. 2013

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Dermorphin is a unique opioid peptide that is 30-40 times more potent than morphine. It was misused and went undetected in horse racing until 2011 when intelligence obtained from a few North American race tracks suggested its use. To prevent such misuse, a reliable analytical method became necessary for detection and identification of dermorphin in post-race horse samples. This paper describes the first liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for such a purpose. Equine plasma and urine samples were pre-treated with ethylenediamine tetra-acetic acid and urea prior to solid-phase extraction (SPE) on Oasis MCX cartridges. Resulting eluates were dried under vacuum and analyzed by LC-MS/MS for dermorphin. The matrix effect, SPE efficiency, intra-day and inter-day accuracy and precision, and stability of the analyte were assessed. The limit of detection was 10 pg/mL in plasma and 20 pg/mL in urine, and the limit of confirmation was 20 pg/mL in plasma and 50 pg/mL in urine. Dermorphin in plasma is stable at ambient temperature, but its diastereomer is unstable. With isotopically labeled dermorphin as an internal standard, the quantification range was 20-10,000 pg/mL in plasma and 50-20,000 pg/mL in urine. The intra-day and inter-day accuracy was from 91 % to 100 % for the low, intermediate, and high concentrations. The intra-day and inter-day coefficients of variation were less than 12 %. The method differentiates dermorphin from its diastereomer. This method is very specific for identification of dermorphin in equine plasma and urine, as assessed by BLAST search and targeted SEQUEST search, and by MS/MS spectrum library search. The method has been successfully applied to analysis of samples collected following dermorphin administration to research horses and of official post-race samples.

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Quantitative determination of oxytocin receptor antagonist atosiban in rat plasma by liquid chromatography–tandem mass spectrometry

Cited by 8 publications

References 18 publications

Estimation of Atosiban Acetate in Parenterals by Rp HPLC

Estimation of Atosiban Acetate in Parenterals by Rp HPLC

Current literature in mass spectrometry

Detection, quantification, and identification of dermorphin in equine plasma and urine by LC–MS/MS for doping control

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