Quantitative determination of phospholipids in sunflower oil

Abstract

Phospholipids from sunflower oil samples were enriched by using solid-phase extraction (SPE) cartridges and subsequently separated and analyzed by high-performance liquid chromatography (HPLC) with an ultraviolet detector. The recovery of individual phospholipids at different total concentrations in model oils and the repeatability of the method were investigated. The results demonstrated the utility of SPE-HPLC for quantitative analysis of phospholipids in sunflower oil and the effectiveness for concentrating, separating, identifying, and quantitating phospholipids in samples with phosphatide contents as low as 0.1%. Samples of sunflower oil at different stages of processing were analyzed, and phospholipid profiles in hexane-extracted oil, hot-pressed oil, and water-degummed oils were compared. JAOCS 74, 511-514 (1997).

Detailed study of Brazil nut (Bertholletia excelsa) oil micro-compounds: phospholipids, tocopherols and sterols

“…23,24 Our experiment shows that Brazil nut oil contains an interesting composition of phospholipid fatty acids comparable to sunflower lecithin. 25,26 Based on these results, Brazil nut oil has the potential to have high commercial value in the health food industry with application in some areas of medical science.…”

“…The different classes of phospholipids, obtained by extraction, were analyzed by HPLC and by spectrophotometry. The percentage was recalculated based on the measured phosphorus content of 377.3 mg L -1 and compared to sunflower lecithin 25 which is the most used phospholipids industrially (Table 4).…”

“…PC content (24%) is half that of the sunflower oil content (47%) whereas phosphatidylserine (PS) is double the amount. 25 Fatty acids extracted from phospholipids were analyzed by FAME/GLC ( Table 5). The results are compared with the results obtained by Smiles et al 26 during the study of the effect of degumming reagents on the recovery and nature of lecithins from crude sunflower oil (Table 6).…”

Identification and Quantification of Natural Lecithin Phospholipids and Their Residuals in Freeze-Dried and Drum-Dried Fruits and Vegetables by LC-MS and HPLC-ELSD

“…the corresponding final product. With regards to lecithin analysis, several methods have historically been used by researchers in order to identify and quantify phospholipids in different sample matrices (Krüger, Bürmannm & Morlock, 2015;Carelli, Brevedan & Crapiste, 1997;Sparace & Moore, 1979;Block, Borne, Joyard & Douce, 1983;Dorne, Joyard, Block & Douce, 1985;Jangle, Galge, Patil & Thorat, 2013;Jungalwala, Evans & McCluer, 1984;McDonald, Robin & Siegel, 1981;Prabha, Raina & Patwardhan, 1988;Robins & Patton, 1986). Thin-layer chromatography, high performance liquid chromatography and nuclear magnetic resonance spectroscopy have been used to analyze phospholipids (Helmerich & Koehler, 2003).…”

Solid phase extraction: Applications to the chromatographic analysis of vegetable oils and fats

“…Phospholipids contribute to the stability and quality of edible oils, fats and fatty foods through their antioxidative activity or contribution to the texture (Singleton and Stikeleather 1995;Carelli et al 1997). On the other hand, they are responsible for oil discoloration during deodorization and steam distillation so that their determination is necessary to evaluate the efficiency of degumming (Mounts and Nash 1990;Mounts et al 1992;Nzai and Proctor 1998).…”