Quantitative determination of protamine using a fluorescent protein chromophore-based AIE probe

A thiol-anchored solvatochromic and fluorogenic molecular rotor for covalent protein labeling in SDS-PAGE and mitochondria specific fluorescence imaging

“…A uorescent dye DASPB with the benzyl group but no benzyl chloride group was also used as the control for spectral, SDS-PAGE and uorescent imaging measurements. The structures of DASPBCl and DASPB were characterized by 1 H NMR, 13 C NMR and ESI-MS (Fig. S1-S6, †).…”

“…The uorescent labeling of proteins with uorescent dyes in SDS-PAGE can be divided into two categories: non-covalent labeling and covalent labeling. The non-covalent labeling is generally achieved through electrostatic or hydrophobic interactions, such as commercial uorescent dye SYPRO protein gel stains 11 and some uorophores, [12][13][14][15] while the covalent labeling method is achieved through chemical reactions between the active groups of uorescent dyes and specic amino acids of proteins. [16][17][18][19] The main advantage of covalent labeling of proteins in SDS-PAGE is that protein samples can be labeled before electrophoresis, which eliminates the tedious gel staining and destaining steps, and proteins can be imaged immediately aer electrophoresis.…”

A chemical biology toolbox to overcome the hypoxic tumor microenvironment for photodynamic therapy: a review

“…8,9 In addition, in recent studies, to enable selective uptake of a drug by tumor cells, photosensitizers are encapsulated into inorganic materials or drug delivery systems, which has become a major trend in the current PDT research. 10 The mechanism of action of PDT is that after the photosensitizer located in the tumor cell absorbs the laser energy of a specific wavelength, it forms an excited triplet state, and then crosses the triplet state of the molecular excited state through intersystem crossing. When it is in the triplet state of the molecular excited state, it can easily carry out energy or electron exchange transfer, forming hydroxyl radicals, singlet oxygen, and other substances.…”

“…8,9 In addition, in recent studies, to enable selective uptake of a drug by tumor cells, photosensitizers are encapsulated into inorganic materials or drug delivery systems, which has become a major trend in the current PDT research. 10…”