Quantitative Determination of Reducing Sugars and a Sugar Acid by Hydroxamic Acid Formation

The inhibition of N-acetyl-β-d-glucosaminase by the (1→4)- and (1→5)-lactones of N-acetylglucosaminic acid

“…This was a modification of the original method of Hestrin (1949). Hilf & Castano (1958) have used a similar method for the determination of reducing sugars after conversion into the sugar acid lactone. To a 0.05% solution of the lactone (2 ml) was added alkaline hydroxylamine (4 ml), which was 1.0 M with respect to hydroxylamine and 1.75M with respect to NaOH.…”

Kinetic Behavior and Mechanism of Inhibition in the Maillard Reaction. III. Kinetic Behavior of the Inhibition in the Reaction Between D‐Glucose and Glycine

“…Gluconic acid formed in the sulfited reaction mixture was determined as gluconic acid hydroxamate (Lien, 1959 ;Hilf and Castano, 1958). Its concentration in the anionic fraction from the sulfited reaction mixture was estimated from a standard calibration curve.…”

“…Its concentration in the anionic fraction from the sulfited reaction mixture was estimated from a standard calibration curve. The kinetics of gluconic acid formation were followed by this method (Hilf and Castano, 1958).…”

Analytical Methods for Carbohydrates

“…256 A drift in end points suggests the presence of a lactone. 261 A very sensitive and specific method for the determination of aldonic and uronic acids has been developed. 257 · 258 Other methods include conversion into sparingly soluble salts, 259 or into copper complexes, 260 which have been used to determine the carboxyl function of aldonic acids.…”