Quantitative determination of rosuvastatin in human plasma by ion pair liquid–liquid extraction using liquid chromatography with electrospray ionization tandem mass spectrometry

A Review of Current Trends and Advances in Analytical Methods for Determination of Statins: Chromatography and Capillary Electrophoresis

“…Assays employing LLE with ethyl acetate [78] and ethyl ether [79] as extraction solvents for determination of rosuvastatin in plasma samples were already published with extraction recoveries of 74 and 69%, respectively. However, in the preliminary study by Lan and co-workers [80], it was found that the extraction recovery of rosuvastatin from plasma in most common organic solvents, such as above mentioned ethyl acetate and ethyl ether, was less than 20%, resulting in an insufficient, imprecise and inaccurate extraction procedure. The authors presumed that low extraction efficacy of rosuvastatin was due to its extremely low water solubility.…”

“…Rosuvastatin has a pyrimidine ring and a carboxylic group in its structure, hence it could be detected either in positive or negative ionization mode. However, the quantification of rosuvastatin in positive ionization mode is more common and was used for determination of rosuvastatin [80] and rosuvastatin together with its metabolites [74], respectively. In the both assays the major ion was protonated molecule [M+H] + in full-scan mode and principal product ion was at m/z 482.…”

“…In some cases one of the statins has been used as internal standard [73,75,77,84], while other works utilized internal standards of various structure, including hydrochlorothiazide [79], estrone [80], naproxen [90], gemfibrozil [91], pioglitazone [95] etc. The best internal standards for precise and accurate quantification in MS or tandem MS are stable-isotopelabeled standards.…”

Bioanalytical method development and validation of simultaneous analysis of telmisartan and hydrochlorthiazide in human plasma using LC-MS/MS

“…No significant interference was observed in the results. Validation Studies [16][17][18][19][20][21][22][23][24] Selectivity: The plasma blank was evaluated six times and the interfering peak was observed at the retention time of the drugs and the retention time of the internal standards. The peaks were of good shapes and the retention time was found to be 3-4 and 1-2 min for HCTZ and telmisartan.…”

Development and Validation of Stability-Indicating HPLC Methods for Quantitative Determination of Pravastatin, Fluvastatin, Atorvastatin, and Rosuvastatin in Pharmaceuticals

“…Several methods based on different techniques have been reported for the analysis of PS, FVS, ATC, and RC in biological fluids. These include high-performance liquid chromatography-mass spectrometry (HPLC=MS) (Gao et al 2007;Lan et al 2007;Borek-Dohalsky et al 2006;Di Pietro et al 2006;Nirogi et al 2006;Oudhoff et al 2006;Xu et al 2006;Hermann, Christensen, and Reubsaet 2005;Singh et al 2005;Trivedi 2005;Zhu and Neirinck 2003) and HPLC (Bahrami et al 2005;Kumar et al 2006;Vittal et al 2006;Um et al 2005;Bauer et al 2005;Zarghi et al 2005;Altuntas and Erk 2004;Al-Rawithi, Hussein, and Alzahrani 2003). Although these methods are sensitive enough to permit their use in determination of PS, FV, ATC, and RC in urine, plasma, and serum, few methods are reported for assay of statins in pharmaceuticals.…”