Quantitative Determination of Serum Triglycerides by the Use of Enzymes

Bucolo, Giovanni; David, H

doi:10.1093/clinchem/19.5.476

Cited by 2,817 publications

(665 citation statements)

References 11 publications

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“…Total T4, T3 and TSH remained essentially unchanged post heparin administration as reported previously by us (McDougall et al, 1982). Serum NEFAs were determined by the method of Falholt et al (1973) and triglycerides by an enzyme colorimetric method (Bucolo & David, 1973) with a Technicon auto analyzer 11.…”

Section: Met Hodssupporting

confidence: 94%

Effect of Heparin on Serum Free Thyroxine Linked to Post‐heparin Lipolytic Activity

Bayer

1983

Clinical Endocrinology

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A possible association between free T4 (fT4) changes, occurring after heparin administration, and the post-heparin lipolytic activity, i.e., the release of lipases and the lipolysis of triglycerides to non-esterified fatty acids (NEFAs) was studied. In 19 patients heparin increased mean fT4 values obtained by equilibrium dialysis from 23.4 to 38.6 pmol/l (P less than 0.01) and mean fT4 values measured by GammaCoat two-step RIA from 17.0 to 24.1 pmol/l (P less than 0.01), the results of the two methods agreeing well (rs = 0.88). Concurrently, NEFAs increased from a mean of 0.55 to 2.20 mmol/l (P less than 0.01) and fT4 increases were significantly correlated with post-heparin NEFAs (rs = 0.69, P less than 0.001). In vitro addition of palmitic acid to sera increased fT4 concentrations to values similar to those observed in vivo. Post-heparin fT4 increases were also strongly correlated to the concentrations of triglycerides (rs = 0.63, P less than 0.01). Hypertriglyceridaemia was associated with pronounced fT4 increases after heparin administration, and normolipidaemia with moderate or no changes. These results suggest that the effect of heparin on fT4 is linked to the activation of lipases by heparin and is mediated by NEFAs, which, at high concentrations, compete with thyroxine for binding proteins.

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Section: Met Hodssupporting

confidence: 94%

Effect of Heparin on Serum Free Thyroxine Linked to Post‐heparin Lipolytic Activity

Bayer

1983

Clinical Endocrinology

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“…Serum cholesterol and triglycerides were assayed enzymically by a Technicon SMAC biochemical analyser (Lie ei al. 1976;Bucolo & David 1973). Lipoprotein fractions were separated electrophoretically and the relative proportions of the lipoprotein fractions assessed on agarose gel (Straw & Wurm 1958).…”

Section: Methodsmentioning

confidence: 99%

Lipids and lipoproteins in women after oophorectomy and the response to oestrogen therapy

Notelovitz

Gudat

Ware

et al. 1983

BJOG

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Summary. The short‐term effects of different types and doses of oestrogen on serum lipids and lipoproteins were studied in 35 oophorectomized women. After 3 months treatment, serum cholesterol levels were unaffected by 1 and 2mg of micronized l7bT‐oestradiol or 0.625 and l.25mg of conjugated equine oestrogens. Triglyceride levels were significantly elevated after treatment with l.25mg of conjugated oestrogens. A trend towards a higher relative proportion of high‐density lipoproteins and a lower relative proportion of low‐density lipoproteins was observed for all four oestrogen regimens, however, statistical significance was not achieved. The proportion of very‐low‐density lipoprotein was unaffected by oestrogen treatment. The age of the oophorectomized women was found to have no effect on either the direction or magnitude of the lipid or lipoprotein responses to oestrogen. Using FSH depression as an index, 1 .25 mg of conjugated oestrogens was found to be the most potent of the four oestrogen regimens tested. Therefore, with respect to lipid balance, little additional clinical benefit is achieved by using a more potent regimen and the risk of adverse side effects may be increased.

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“…The total leukocytes count (WBCs count; 10 3 ml −1 ) and differential leukocyte counts were determined using the method described by Dacie and Lewis (1991). The serum samples were used to estimate the serum glucose, cholesterol and triglycerides levels colorimetrically according to the previous methods (Allain, Poon, Chan, Richmond, & Fu, 1974;Buccolo & David, 1973;Trinder, 1969) respectively.…”

Section: Haematological and Biochemical Indicesmentioning

confidence: 99%

Growth performance, haematology and intestinal histo‐morphology of Nile tilapia fed on Indian Lotus (Nelumbo nuciferaGaertn.) leaf powder at different concentrations

2019

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Indian lotus, Nelumbo nucifera (Gaertn.) is a valued medicinal plant that exhibits several pharmacological properties. The present work aimed to investigate the effect of Indian lotus as a feed supplement on the growth performance, haematological and biochemical indices, and intestinal histo‐morphology of Nile tilapia (Oreochromis niloticus L.). The fish were randomly distributed into four groups and fed on a basal diet containing Indian lotus leaf powder at different concentrations (0, 0.1, 0.2 and 0.4%) for up to 60 days. The results elucidated that Indian lotus supplemented diets (0.2% and 0.4% followed by 0.1%) significantly improved weight gain, specific growth rate (%) and feed conversion ratio (p < .05). The feed intake was significantly increased in the fish fed on the Indian lotus supplemented diets in a dose‐dependent manner (p < .05). However, Indian lotus had no significant effect (p > .05) on survival rate, total erythrocytes (RBCs) count, haemoglobin (Hb) and hematocrit value (PCV, %) except a significant reduction on Hb content of the fish fed on 0.2% Indian lotus and a significant increase in PCV (%) in the fish fed on 0.1% Indian lotus (p < .05). The total leucocytes (WBCs), neutrophils and lymphocytes counts displayed significant elevations in the fish fed on the Indian lotus diets especially at 0.2% and 0.4%, whereas significant reduction in neutrophils count in the fish fed on 0.1% Indian lotus (p < .05) was observed. The serum glucose was significantly decreased in the fish fed on Indian lotus (0.1% and 0.2%), whereas cholesterol and triglycerides were markedly increased in the fish fed on the Indian lotus supplemented diets (p < .05). Moreover, the intestinal villous heights and the numbers of goblet cells and intraepithelial lymphocytes (IEL) were significantly boosted in all parts of the intestine in all Indian lotus diet groups as compared with the control group. However, as an exception, villous heights in the middle part of the intestine in the fish fed on 0.1% and 0.4% diets (p < .05) were not affected. In the proximal part, the villous heights and the numbers of goblet cells were markedly increased in fish fed on 0.2% and 0.4% diets followed by 0.1% diet. The fish fed on 0.2% lotus supplemented diet followed by 0.1% and 0.4% supplemented diets exhibited significant elevations in villous heights in the distal part and the numbers of goblet cells in the middle part of the intestine. There were no significant differences between the fish fed on the supplemented diets in the numbers of goblet cells in the distal part and the numbers of IEL in the proximal and middle parts (p > .05). In the distal part, the numbers of IEL revealed a significant elevation in 0.4% lotus supplemented diet followed by 0.1% and 0.2% lotus supplemented diet groups (p < .05). Therefore, this study indicates the advantageous effect of the Indian lotus leaves as a natural feed additive for improving growth, intestinal structure and hence, health status of Nile tilapia.

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Quantitative Determination of Serum Triglycerides by the Use of Enzymes

Cited by 2,817 publications

References 11 publications

Effect of Heparin on Serum Free Thyroxine Linked to Post‐heparin Lipolytic Activity

Effect of Heparin on Serum Free Thyroxine Linked to Post‐heparin Lipolytic Activity

Lipids and lipoproteins in women after oophorectomy and the response to oestrogen therapy

Growth performance, haematology and intestinal histo‐morphology of Nile tilapia fed on Indian Lotus (Nelumbo nuciferaGaertn.) leaf powder at different concentrations

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