Quantitative Determination of Steroidal Saponins from Dioscorea Species and Dietary Supplements using UHPLC-PDA-ELSD

Avula, Bharathi; Wang, YH; Wang, M; Ali, Z; Smillie, TJ; Khan, IA

doi:10.1055/s-0033-1336571

Cited by 7 publications

(7 citation statements)

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“…The later one is a trifunctionally bonded alkyl column, which can impact the retention, selectivity and sensitivity of ionizable compounds, thus has been used to separate PFAAs extensively [7,30,31,33]. The former one contains an embedded-polar group that combines the hydrophobicity of a straight-chain-alkyl ligand (C18) with the hydrophilicity of an embedded polar group (carbamate), which improves the retention of and peak shape of polyhydric substances [40,41]. Two ACQUITY UPLC BEH Shield RP18 columns with different lengths (50 mm × 2.1 mm and 150 mm × 2.1 mm) were compared and it was found that the longer column (150 mm × 2.1 mm) had both higher separation efficiency and better peak shape.…”

Section: Chromatograms Of Ftohs and Poly-and Perfluorinated Acidsmentioning

confidence: 99%

Determination of fluorotelomer alcohols and their degradation products in biosolids-amended soils and plants using ultra-high performance liquid chromatography tandem mass spectrometry

Zhang

Wen

et al. 2015

Journal of Chromatography A

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a b s t r a c tDegradation of fluorotelomer alcohols (FTOHs) was recognized as an additional source of perfluorocarboxylic acids (PFCAs). Quantification of FTOHs and their degradation products can help shed light on the sources and fates of PFCAs in the environment. In this study, an analytical method was developed for the determination of 6:2 and 8:2 FTOHs, and their degradation products of poly-and perfluorinated acids, including fluorotelomer saturated and unsaturated carboxylic acids (FTCAs and FTUCAs), secondary polyfluorinated alcohols and PFCAs in biosolids-amended soils and plants using ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The extract efficiencies of different methods including ethyl acetate and methanol (MeOH) for FTOHs and acetonitrile, MeOH, methyl tertbutyl ether (MTBE), NaOH-MeOH and NaOH-MTBE for poly-and perfluorinated acids were tested. The results showed that 6:2 and 8:2 FTOHs and their degradation products could be simultaneously and satisfactorily extracted by MeOH, cleaned up by Envi-Carb graphitized carbon and solid phase extraction, respectively, and determined by UPLC-MS/MS separately. NaOH in the extractant caused the conversion of 6:2 FTCA and 8:2 FTCA into the corresponding FTUCAs. The selected methods have matrix recoveries ranged from 52% to 102%, and detection limits of 0.01-0.46 ng/g dry weight for FTOHs and their degradation products in soil and plant. The optimized method was applied successfully to quantify FTOHs and their degradation products in two biosolids-amended soils and plants. The total concentrations of FTOHs in the soils were 44.1 ± 5.8 and 82.6 ± 7.1 ng/g, and in plants tissues 3.58 ± 0.25 and 8.33 ± 0.66 ng/g. The total concentrations of poly-and perfluorinated acids in the soils were 168.0 ± 13.2 and 349.6 ± 11.2 ng/g, and in plants tissues 78.0 ± 6.4 and 75.5 ± 5.3 ng/g.

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Section: Chromatograms Of Ftohs and Poly-and Perfluorinated Acidsmentioning

confidence: 99%

Determination of fluorotelomer alcohols and their degradation products in biosolids-amended soils and plants using ultra-high performance liquid chromatography tandem mass spectrometry

Zhang

Wen

et al. 2015

Journal of Chromatography A

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“…These techniques offer a significant advantage for rapid screening of target compounds in complex matrices. In our previous studies, the quantification of steroidal saponins from D. villosa and Dioscorea species has been reported [30]. In the present study, the structural characteristics of steroidal saponins from methanolic extracts of dried rhizomes, the roots of Dioscorea species (D. villosa and D. cayenensis), and dietary supplements have been studied using UHPLC-QTOF-MS in both negative and positive ion modes.…”

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confidence: 94%

Characterization of Steroidal Saponins from Dioscorea villosa and D. cayenensis Using Ultrahigh Performance Liquid Chromatography/Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry

Avula

Wang

et al. 2014

Planta Med

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Yam (Dioscorea spp.) is an important edible tuber plant used for medicinal purposes to promote health and longevity in Chinese tradition. Steroidal saponins were reported to be the major physiologically active constituents in yams. In this current work, the structural characteristics of steroidal saponins in methanolic extracts from dried rhizomes of two Dioscorea species (D. villosa L. and D. cayenensis Lam.) and dietary supplements have been identified and analyzed using UHPLC/QTOF-MS in both negative and positive ion modes. The fragmentation patterns of reference standards were determined and the steroidal saponins in the extracts were identified or tentatively characterized from their retention times and mass spectra. The fragments produced by collision-induced dissociation (CID) revealed the characteristic cleavage of glycosidic bonds, and the fragmentation pattern provided structural information about the sugars. Twenty-one saponins, including four tentatively identified compounds, were detected in the crude extracts of two Dioscorea species. These saponins can be used to distinguish D. villosa from D. cayenensis. For example, asperin and gracillin are found only in D. cayenensis, and dioscoreavilloside A and B and parvifloside are only found in D. villosa. This can be used to determine the presence or absence of D. villosa in commercial products, which may help determine the spiking of plant material, and/or prevent the use of potentially mislabeled or misidentified “Dioscorea” material. The analytical method also provided an alternative, fast method for quality control of Dioscorea species in dietary supplements. Principal component analysis showed that Dioscorea species and commercial products were easily distinguished. From this a partial least squares model was constructed to determine what species are in different products.

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“…Many natural products derived from herbs exhibit a wide range of pharmacological properties, including the antimalarial drug artemisinin from Artemisia apiacea [9], the anticancer drug paclitaxel from Taxus brevifolia [10], and quercetin found in various vegetables and fruits [11]. Diosgenin (DG) is a naturally occurring steroidal sapogenin isolated from Agavaceae, Dioscoreaceae, Liliaceae, Solanaceae, Scrophulariaceae, Amaryllidaceae, Leguminosae, and Rhamnaceae [12][13][14][15][16][17]. It has been extensively studied for the management and treatment of different types of cancer [18], osteoporosis [19], cardiovascular diseases [20], atherosclerosis [21], diabetes mellitus [22], and skin diseases [23].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Diosgenin and Its Major Derivatives against Neurological Diseases: Recent Advances

Cai

Zhang

Wang

et al. 2020

Oxidative Medicine and Cellular Longevity

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Diosgenin (DG), a well-known steroidal sapogenin, is present abundantly in medicinal herbs such as Dioscorea rhizome, Dioscorea villosa, Trigonella foenum-graecum, Smilax China, and Rhizoma polgonati. DG is utilized as a major starting material for the production of steroidal drugs in the pharmaceutical industry. Due to its wide range of pharmacological activities and medicinal properties, it has been used in the treatment of cancers, hyperlipidemia, inflammation, and infections. Numerous studies have reported that DG is useful in the prevention and treatment of neurological diseases. Its therapeutic mechanisms are based on the mediation of different signaling pathways, and targeting these pathways might lead to the development of effective therapeutic agents for neurological diseases. The present review mainly summarizes recent progress using DG and its derivatives as therapeutic agents for multiple neurological disorders along with their various mechanisms in the central nervous system. In particular, those related to therapeutic efficacy for Parkinson’s disease, Alzheimer’s disease, brain injury, neuroinflammation, and ischemia are discussed. This review article also critically evaluates existing limitations associated with the solubility and bioavailability of DG and discusses imperatives for translational clinical research. It briefly recapitulates recent advances in structural modification and novel formulations to increase the therapeutic efficacy and brain levels of DG. In the present review, databases of PubMed, Web of Science, and Scopus were used for studies of DG and its derivatives in the treatment of central nervous system diseases published in English until December 10, 2019. Three independent researchers examined articles for eligibility. A total of 150 articles were screened from the above scientific literature databases. Finally, a total of 46 articles were extracted and included in this review. Keywords related to glioma, ischemia, memory, aging, cognitive impairment, Alzheimer, Parkinson, and neurodegenerative disorders were searched in the databases based on DG and its derivatives.

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Quantitative Determination of Steroidal Saponins from Dioscorea Species and Dietary Supplements using UHPLC-PDA-ELSD

Cited by 7 publications

References 0 publications

Determination of fluorotelomer alcohols and their degradation products in biosolids-amended soils and plants using ultra-high performance liquid chromatography tandem mass spectrometry

Determination of fluorotelomer alcohols and their degradation products in biosolids-amended soils and plants using ultra-high performance liquid chromatography tandem mass spectrometry

Characterization of Steroidal Saponins from Dioscorea villosa and D. cayenensis Using Ultrahigh Performance Liquid Chromatography/Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry

Therapeutic Potential of Diosgenin and Its Major Derivatives against Neurological Diseases: Recent Advances

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