Quantitative determination of the major saponin mixture bacoside A in Bacopa monnieri by HPLC

Abstract

Bacoside A, the putative bioactive component of the Indian medicinal plant Bacopa monnieri, was found to be a mixture of saponins with bacoside A3 (1), bacopaside II (2), jujubogenin isomer of bacopasaponin C (3) and bacopasaponin C (4) as major constituents. An HPLC method together with an optimised extraction procedure was developed for the estimation of 1-4 in B. monnieri to enable standardisation of the latter. Concentration ranges of the analytes in samples of B. monnieri collected from different regions of India were 0.14-0.85% (w/w) (1), 0.12-0.69% (2), 0.05-0.72% (3) and 0.05-0.44% (4). The importance of using bacoside A, with known concentrations of 1-4, as a reference standard for the routine analysis of B. monnieri is highlighted. Two common flavonoids, luteolin and apigenin, were present in all samples of B. monnieri.

Effects of a Standardized Bacopa monnieri Extract on Cognitive Performance, Anxiety, and Depression in the Elderly: A Randomized, Double-Blind, Placebo-Controlled Trial

“…28 Chemical characterizations of the plant have been carried out by many investigators. [29][30][31][32][33][34][35][36][37][38][39][40] There are no data currently available on the pharmacokinetics of Bacopa or its saponins. Phase I clinical studies confirmed the safety of bacosides in healthy male volunteers at both single and chronic dosing administered over a period of 4 weeks 7 and 6 weeks with a dose of up to 450 mg of dried water extract.…”

Estimation of Twelve Bacopa Saponins in Bacopa monnieri Extracts and Formulations by High-Performance Liquid Chromatography

“…Ganzera et al 15) reported the method for quantification of six saponins using gradient HPLC with PDA detector, but one of the major peaks was not completely characterized. Deepak et al 16) determined bacoside A which contains 4 compounds by HPLC, but did not consider bacoside B. In another report, bacosides were estimated by HPLC, coupled NMR, MS and bioassay methods described.…”

“…A high performance thin-layer chromatographic (HPTLC) 11) method was developed for the estimation of bacoside A in Bacopa monnieri plant and its formulations. A few high performance liquid chromatographic (HPLC) [12][13][14][15][16] methods were also developed for the quantification of bacosides in Bacopa monnieri extracts and formulations. Ganzera et al 15) reported the method for quantification of six saponins using gradient HPLC with PDA detector, but one of the major peaks was not completely characterized.…”

Effect of Bacopa monniera Linn. extract on murine immune response in vitro

“…Two common flavonoids, luteolin and apigenin, were also presented (Deepak et al, 2005). Our HPLC analysis showed that the bacoside A contained in B. monniera extract was approximately 0.6% (w/w), which was in the same range as reported previously (Deepak et al, 2005).…”