Quantitative Determination of the Radical Scavenging Activity of Antioxidants in Black Tea Combined with Common Spices Using Ultraviolet-Visible Spectroscopy

Yuan, Xianglin; Wang, Chaoqiuyu; Perera, Manori

doi:10.1080/00032719.2022.2098309

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…Therefore, it is imperative to determine the antioxidant activity capacity of D. officinale to safeguard consumers’ interests. However, the common analytical methods for bioactive substance are always complicated, time-consuming, and laborious, waste reagents, and pollute the environment, mainly including ultraviolet–visible spectrophotometry (UV-Vis) and high-performance liquid chromatography (HPLC) [ 7 , 8 , 9 , 10 ]. Therefore, it is necessary to develop a more efficient, environmentally friendly, and convenient method to assess the antioxidant activity of D. officinale .…”

Section: Introductionmentioning

confidence: 99%

Rapid Measurement of Antioxidant Properties of Dendrobium officinale Using Near-Infrared Spectroscopy and Chemometrics

Cao,

Huang,

Chen

et al. 2024

Foods

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Dendrobium officinale (D. officinale), often used as a dual-use plant with herbal medicine and food applications, has attracted considerable attention for health-benefiting components and wide economic value. The antioxidant ability of D. officinale is of great significance to ensure its health care value and safeguard consumers’ interests. However, the common analytical methods for evaluating the antioxidant ability of D. officinale are time-consuming, laborious, and costly. In this study, near-infrared (NIR) spectroscopy and chemometrics were employed to establish a rapid and accurate method for the determination of 2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity, and ferric reducing antioxidant power (FRAP) in D. officinale. The quantitative models were developed based on the partial least squares (PLS) algorithm. Two wavelength selection methods, namely the genetic algorithm (GA) and competitive adaptive reweighted sampling (CARS) method, were used for model optimization. The CARS-PLS models exhibited superior predictive performance compared to other PLS models. The root mean square errors of cross-validation (RMSECVs) for ABTS, FRAP, and DPPH were 0.44%, 2.64 μmol/L, and 2.06%, respectively. The results demonstrated the potential application of NIR spectroscopy combined with the CARS-PLS model for the rapid prediction of antioxidant activity in D. officinale. This method can serve as an alternative to conventional analytical methods for efficiently quantifying the antioxidant properties in D. officinale.

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Section: Introductionmentioning

confidence: 99%

Rapid Measurement of Antioxidant Properties of Dendrobium officinale Using Near-Infrared Spectroscopy and Chemometrics

Cao,

Huang,

Chen

et al. 2024

Foods

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“…However, ROS’s excessive or insufficient free radical scavenging capacity in vivo will lead to cell membrane damage and promote cell death ( 8 ). Intake of antioxidants benefits human health because they impede the level of ROS and free radicals ( 9 ). The immunoregulatory activity of EPS in LAB is mainly due to the production of interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α), and phagocytosis of macrophages ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

Structural, antioxidant, and immunomodulatory activities of an acidic exopolysaccharide from Lactiplantibacillus plantarum DMDL 9010

Huang

et al. 2022

Front. Nutr.

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This study investigated the structural, antioxidant, and immunomodulatory activities of acidic exopolysaccharide (EPS-LP2) isolated from Lactiplantibacillus plantarum DMDL 9010. EPS-LP2 is composed of fucose (Fuc), arabinose (Ara), galactose (Gal), glucose (Glc), mannose (Man), and D-fructose (Fru) with a molar ratio of 0.13: 0.69: 8.32: 27.57: 62.07: 0.58: 0.46, respectively. Structural analysis of EPS-LP2 exhibited a smooth irregular lamellar surface, rod-like structure with swollen ends and slippery surfaces, and good thermal stability. Based on the methylation and NMR analysis, sugar residues including t-Manp, t-Glcp, 2-Manp, 6-Galp, 6-Glcp, and 4-Glcp were found to exist in EPS-LP2. In the 50∼400 μg/ml range, EPS-LP2 showed negligible neurotoxicity to RAW264.7 cells. Moreover, EPS-LP2 could protect RAW264.7 cells from oxidative injury by lowering the generation of reactive oxygen species (ROS), malondialdehyde (MDA), and the secretion of lactate dehydrogenase (LDH). In contrast, an increase in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and the concentrations of glutathione (GSH) were observed. Immunoreactivity assays showed that EPS-LP2 could suppress the expression of NO, tumor necrosis factor-α (TNF-α), and interleukin 6 (IL-6) and inhibit the activation of the mitogen-activated protein kinase (MAPK)/nuclear factor-κB-gene binding (NF-κB) cell pathway. Conclusively, EPS-LP2 could be a potential natural antioxidant and immunomodulatory agent in functional foods and medicines.

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Quantitative Determination of the Radical Scavenging Activity of Antioxidants in Black Tea Combined with Common Spices Using Ultraviolet-Visible Spectroscopy

Cited by 2 publications

References 32 publications

Rapid Measurement of Antioxidant Properties of Dendrobium officinale Using Near-Infrared Spectroscopy and Chemometrics

Rapid Measurement of Antioxidant Properties of Dendrobium officinale Using Near-Infrared Spectroscopy and Chemometrics

Structural, antioxidant, and immunomodulatory activities of an acidic exopolysaccharide from Lactiplantibacillus plantarum DMDL 9010

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