Quantitative determination of valproic acid by means of gas chromatographie headspace analysis

Degel, Fritz; Heidrich, Robert; Schmid, R; Weidemann, Gerhard

doi:10.1016/0009-8981(84)90189-x

Cited by 7 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there are doubts about the need for monitoring phenobarbitone and carbamazepine (Richens, 1982), serum drug measurements are generally considered essential in the use of phenytoin, for polypharmaceutical treatment and for patients with renal or hepatic diseases (Richens, 1982;Chadwick, 1983). AED's can now be measured by many different techniques such as enzyme immunoassay (EMIT) (Gonzales et al, 1983), fluorescent immunoassay (Gonazlez et al, 1983), GLC (Sarhan et al, 1980;Degel et al, 1984) and HPLC (Adams et al., 1978;Gerson et al, 1984). Among these methods EMIT and HPLC seem to be the most widely used.…”

Section: Introductionmentioning

confidence: 98%

Automated and routine liquid chromatographic analysis of antiepileptic drugs

Pang

Cheung

1986

Biomedical Chromatography

View full text Add to dashboard Cite

We describe an optimized automated liquid chromatographic method for simultaneous measurement of primidone, phenobarbitone, phenytoin, carbamazepine and clonazepam. A Waters Tri-Module automation system is used and it provides direct read-out of results after chromatography. A one-step extraction with ethyl acetate is used to extract the drugs from 100 microL serum samples. We use an isocratic mobile phase and monitor the column effluent at 210 nm. Drug levels as low as 5 mumol/L can be detected. The within-run CV's range from 1.4 to 2.7%, and the between-run CV's range from 5.2 to 6.1%. Analytical recovery is in the range from 94-108%. The method compares favourably with the enzyme multiple immunoassay technique for routine antiepileptic drugs monitoring, in accuracy, efficiency and cost-effectiveness.

show abstract

Section: Introductionmentioning

confidence: 98%

Automated and routine liquid chromatographic analysis of antiepileptic drugs

Pang

Cheung

1986

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Additionally, due to VPA volatility, GC is often used. 20,21 In order to prevent severe tailing of the fatty acid peak, on-column and pre-column derivatization has also been used. 22 Because VPA is a relatively strong organic acid, it does not chromatograph well on the standard nonpolar or slightly polar stationary phases routinely applied to clinical GLC determination of other anticonvulsant drugs unless first derivatized.…”

Section: Introductionmentioning

confidence: 99%

A rapid and simple procedure for monitoring valproic acid by gas chromatography

2018

View full text Add to dashboard Cite

Valproic acid (VPA), a widely used antiepileptic drug, has a narrow therapeutic range of 50-100 μg/mL and shows large individual variability. It is very important to monitor the trough VPA concentration using a reliable method. The aim of this study was to develop and validate a rapid gas chromatographic (GC) technique for VPA quantification in human plasma and to compare it with the traditional immunoassay method. VPA extraction from human serum was efficient by dichloromethane and hydrochloric acid using octanoic acid as an internal standard. GC analysis was performed using a gas-chromatograph equipped with a flame ionization detector (GC/FID). VPA detection and quantification were accomplished isothermally at 135°C on a Gs-BP 100% dimethylpolysiloxane capillary column (10 m×0.53 mm ID, 2.65 μm film thickness, Supelco, Bellefonte, PA). Injection port and detector temperature were 280°C. Retention times of VPA and internal standard were 1.83 min and 2.33 min, respectively. The calibration curve was linear over the concentration range of 5-320 μg/mL, with a lower limit of detection of 1.25 μg/mL. The internal and inter-day precision was less than 5.3% and 6.1%, respectively, and the accuracy was below 2.8%. VPA recovery was 94.6%. A quick and accurate method for VPA determination in human plasma was developed and validated. It resulted sufficiently selective and sensitive.

show abstract

Determination of antiepileptic drugs

Burke¹,

Thénot²

1985

Journal of Chromatography B: Biomedical Sciences and Applicatio

View full text Add to dashboard Cite

Quantitative determination of valproic acid by means of gas chromatographie headspace analysis

Cited by 7 publications

References 21 publications

Automated and routine liquid chromatographic analysis of antiepileptic drugs

Automated and routine liquid chromatographic analysis of antiepileptic drugs

A rapid and simple procedure for monitoring valproic acid by gas chromatography

Determination of antiepileptic drugs

Contact Info

Product

Resources

About