Quantitative Determination of Verbascoside in<i>Scrophularia scorodonia</i>. by High-Performance Liquid Chromatography

Fernández, L.; Santos, Javier de; Dı́az-Lanza, Ana Marı́a; Perez, Enrique Pardo; Villaescusa, Lucinda

doi:10.1080/13880200590928799

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…One group of aromatic protons belongs to the caffeic acid replacement, while others belong to the phenylethanoid structure. The structure of verbascoside is well known in many reviewed literature (232425); our data are in good conformity with aforementioned studies. Existence of characteristic and typical peaks at δ 6.20 ( d , 1H, J = 15.86 Hz) and δ 7.45 ( d , 1H, J = 15.73 Hz) is the main cause for presence of phenyl propanoid structure.…”

Section: Discussionsupporting

confidence: 92%

“…Among the protons of glucose, H-4 glucose showed higher shift at 4.68 ppm, which proved the connection of caffeic acid substitution on C-4 glucose. 13 C-NMR spectrum of verbascoside was completely in agreement with published data (232425).…”

Section: Discussionsupporting

confidence: 88%

“…Verbascoside (1) is a famous phenylpropanoid which has been isolated and elucidated from the aerial parts and roots of many Scrophularia species (111225). Based on our knowledge, this is the first report on verbascoside presence in the aerial parts of S. subaphylla .…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical analysis and antiproliferative activity of the aerial parts of Scrophularia subaphylla

et al. 2019

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Scrophularia subaphylla ( S. subaphylla ) L., a medicinal plant from the Scrophulariaceae family, has been reported to possess potential profits in the treatment and prophylaxis of different diseases. Some phenolic compounds in this genus have been displayed decent effects on different types of cancer via multiple mechanisms. The current study aimed to bioassay guided isolation of cytotoxic constituents from the aerial parts of S. subaphylla against breast (MCF-7) and colon (HT-29) cancer cell lines as well as normal cells (L929). Different extracts of S. subaphylla were acquired by Soxhlet apparatus and then subjected to brine shrimp lethality test and MTT assay for assessing their cytotoxic characteristics. Cytotoxic extract subjected to further phytochemical fractionation using solid phase extraction, reversed-phase high pressure liquid chromatography (RP-HPLC), and one dimensional nuclear magnetic resonance (1D-NMR) spectroscopy. The biological activity of the isolated pure components, verbascoside and 3‘ O rhamnosyl -4‘ O para coumaryl 7- hydroxyl salidroside , was assessed using MTT assay against MCF-7 and HT-29 carcinoma cells. Two known phenylpropanoid compounds were isolated from this species. Their structures were elucidated by spectroscopic data (using 1 H-NMR and 13 C-NMR) and compared with the previous literature. Both pure compounds in comparison with control group demonstrated significant antiproliferative activity against cancerous cells ( P < 0.001). In our study, verbascoside and its derivative could inhibit proliferation of cancerous cells without any side effects on normal cells.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

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Phytochemical analysis and antiproliferative activity of the aerial parts of Scrophularia subaphylla

et al. 2019

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“…In acteoside and pedaliin, the commercial reagent or isolate from plant material were used as a reference standard in HPLC and LC-MS/MS analysis for quantification [21][22][23][24][25][26]. However, Table 1.…”

Section: Quantification Of Acteoside and Pedaliin In Dried Sesame Leamentioning

confidence: 99%

Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods

et al. 2020

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A high-performance liquid chromatography (HPLC) method with relative molar sensitivity (RMS) based on 1H quantitative NMR spectroscopy (1H-qNMR) has been developed for food ingredients such as acteoside (verbascoside) and pedaliin (pedalitin-6-O-glucoside) without requiring authentic and identical standards as the reliable analytical methods. This method is used methyl 4-hydroxybenzoate (MHB) as an alternative reference standard. Each RMS is also calculated from the ratio of each analyte's molar absorption coefficient to that of MHB after correcting the purities of the analytes and reference standard by 1H-qNMR. Therefore, this method can quantify several analytes with metrological traceability to the International System of Units (SI) using the RMS and one alternative reference standard. In this study, the content of acteoside and pedaliin in several samples, such as dried sesame leaf powders and commercially processed foods, can be determined by the proposed RMS method and demonstrated in good agreement that obtained by a conventional method. Moreover, the proposed method yields analytical data with SI-traceability without the need for an authentic and identical analyte standard. Thus, the proposed RMS method is a useful and practical tool for determining acteoside and pedaliin in terms of the accuracy of quantitative values, the routine analysis, and the cost of reagents.

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“…In general, the phytochemical compounds herein found in the two S. tenuipes fractions are in line with previous reported data for Scrophularia plants, since phenylethanoid/phenylpropanoid glycosides and iridoids represent their major metabolites. In this regard, martynoside has been previously isolated from S. dentata [1], S. xanthoglossa [24], S. koraiensis [25], and S. umbrosa [26], while the iridoid harpogoside was reported for S. ningpoensis [6], S. scorodonia [7,27], S. buergeriana [8], and S. nodosa [5]. Additionally, caffeic acid, coumaric acid derivatives, and kaempferol derivatives were also previously detected in Scrophularia plants.…”

Section: Phenolic Compounds Of Ethyl Acetate (Ea) and N-butanol (Bu) mentioning

confidence: 99%

Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities

et al. 2020

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Scrophularia tenuipes is an Algerian-Tunisian endemic species, which has not been studied yet. Ethyl acetate (EA) and n-butanol (Bu) fractions obtained from Scrophularia tenuipes were investigated for their health benefit properties, in particular with respect to in vivo/in vitro anti-inflammatory and antioxidant activities, as well as their potential to inhibit key enzymes with impact in diabetes (α-glucosidase and α-amylase). The fractions had a distinct phytochemical composition, of which EA was richer in total phenolic compounds (225 mg GAE/g) and mostly composed of the phenylethanoid acetyl martynoside. Compared to EA, Bu had higher amounts of total flavonoids, and according to the result obtained from UHPLC-DAD-ESI-MSn analysis, harpagoside (iridoid) was its major phytochemical. EA fraction was quite promising with regard to the in vivo (at 200 mg/kg, po) anti-inflammatory effect (62% and 52% for carrageenan-induced rat paw edema and xylene-induced ear edema tests, respectively), while Bu fraction exhibited a stronger antioxidant capacity in all tests (IC50 = 68 µg/mL, IC50 = 18 µg/mL, IC50 = 18 µg/mL and A0.50 = 43 µg/mL for DPPH●, ABTS•+, O2•− scavenging assays and cupric-reducing antioxidant capacity method, respectively). Both fractions also showed a strong effect against α-amylase enzyme (IC50 = 8 µg/mL and 10 µg/mL for EA and Bu fraction, respectively).

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Quantitative Determination of Verbascoside inScrophularia scorodonia. by High-Performance Liquid Chromatography

Cited by 6 publications

References 12 publications

Phytochemical analysis and antiproliferative activity of the aerial parts of Scrophularia subaphylla

Phytochemical analysis and antiproliferative activity of the aerial parts of Scrophularia subaphylla

Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods

Scrophularia Tenuipes Coss and Durieu: Phytochemical Composition and Biological Activities

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