QUANTITATIVE DETERMINATION OF VITAMIN G(B₂)

Abstract

Our purpose in the work here described2 has been to develop a method whereby the responses in the weight curves of properly standardized experimental animals may become means of measuring the relative vitamin G (B2) values of foods or of concentrates obtained in research work.The factor here designated as vitamin G or B2 may or may not be identical with that to which the term pellagra-preventive has been applied; 1 Published as Contribution No. 667 from the Department of Chemistry of Columbia University.* This paper is a brief summary of the work described in the privately printed dissertation submitted by Anne Bourquin in partial fulfilment of the requirements for the degree of Ph.D., Columbia University, 1929, together with the results of subsequent experience in the use of the method in the Columbia laboratories.

Experimental Study of Clinical Vitamin B Deficiency 1

“…The vitamin B1 substance 4 was prepared as an 80 per cent alcoholic extract of wheat embryo according to the method of Bourquin and Sherman (5). Using the rat growth method of biological assay (6), this extract was found to be free of other vitamin fractions and to be potent in vitamin B1, 1 cc.…”

“…for the remainder of the experiment. Powdered brewer's yeast concentrate 5 prepared by a modification of the Osborne and Wakeman method (8) and known to contain factors in addition to vitamins B1 and B2 necessary for maximal growth in the rat ( Figure 2) was used as a source of additional B factors. An arbitrary dose of 6 tablespoonfuls was given daily.…”

American Public Health Association Year Book 1940-1941

“…In Sept., 1924, Apr., 1925, Feb., 1926, July, 1927, Apr., 1928, Feb., 1929, May, 1930, Year Books 1930-1931, 1931-1932, 1932, 1934, 1937-1940 [75] There is need for extending control over water distributing systems to include house connections, service piping, and water connections to plumbing fixtures, and there is much merit to the idea that water supply authorities should have control over these matters.…”

“…1930, Year Books 1930-1931, 1931-1932-1936 Considerable variation in recommendations for quality of water for outdoor bathing waters is found, but most authorities seem to favor a limiting B. coli index of 50 per 100 cc. This same value finds favor as a limiting value for approved shellfish growing areas.…”

“…growth method of Chase and Sherman (1931). In addition to the above two biological methods, there have been proposed (1) a protective, (2) a weight maintenance, and (3) a curative pigeon procedure, as well as an electrocardiographic bradycardia method with rats.…”