Quantitative Estimation of Lipid Peroxidation and Antioxidants Enzymes in the Diagnosis and Prognosis of Gastrointestinal Carcinoma

Abstract

ABSTRACT:The present study mainly highlights the occurrence of lipid peroxidation and possible breakdown of antioxidants in gastrointestinal carcinoma patients as compared to healthy control subjects. Enzymatic and non-enzymatic parameters play an important role in cell protection against harmful influence of oxidative stress. Our aim of the present study was to investigate the levels of final lipid peroxidation products like (MDA) malondialdehyde and activity of antioxidative enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in gastrointestinal carcinoma. The study consisted of 40 diagnosed gastrointestinal carcinoma patients and an equal number of age and sex matched healthy control subjects. The Activity of MDA, glutathione peroxidase and superoxide dismutase were determined by spectrophotometric method. From this study, we observed that the MDA were increased significantly & antioxidants were altered highly significantly. Therefore, for the diagnosis of human gastric cancer or recurrence, the study of lipid peroxidation & antioxidant defense mechanism may be useful tool along with the biopsy, endoscopy & Pathological investigations.

Evaluation and Monitoring of Superoxide Dismutase (SOD) Activity and its Clinical Significance in Gastric Cancer: A Systematic Review and Meta-Analysis

“…The database search initially identified 574 publications through the database search, and ten studies were finally identified after meeting all inclusion criteria and requirements [ 13 – 22 ]. The ten published studies included 1,094 subjects, 495 patients with gastric cancer and 599 healthy individuals [ 13 – 22 ]. Table 1 shows the baseline characteristics of these subjects included in the ten studies, which were published from 1993–2015 [ 13 – 22 ].…”

“…The ten published studies included 1,094 subjects, 495 patients with gastric cancer and 599 healthy individuals [ 13 – 22 ]. Table 1 shows the baseline characteristics of these subjects included in the ten studies, which were published from 1993–2015 [ 13 – 22 ]. Five controlled studies [ 13 , 15 , 16 , 20 , 22 ] included an equal number of patients with gastric cancer and normal subjects who were age-matched and sex-matched and who had not undergone any treatment before providing blood samples.…”

“…Table 1 shows the baseline characteristics of these subjects included in the ten studies, which were published from 1993–2015 [ 13 – 22 ]. Five controlled studies [ 13 , 15 , 16 , 20 , 22 ] included an equal number of patients with gastric cancer and normal subjects who were age-matched and sex-matched and who had not undergone any treatment before providing blood samples. Among these five studies, Arivazhagan et al [ 15 ] and Pasupathi et al [ 20 ] enrolled only men as the study cases and controls.…”

“…One study [ 18 ] was part of the Japan Collaborative Cohort Study (JACC), which included 210 participants (110 men and 100 women), in which each patient with gastric cancer had age-matched and sex-matched controls, with matched controls for age at recruitment, and research site, and with sufficient baseline blood samples were reserved for study. According to the Newcastle-Ottawa Scale (NOS) scores, five articles [ 13 , 15 , 18 , 20 , 22 ] were of high quality and five studies [ 14 , 16 , 17 , 19 , 21 ] were scored as medium quality. Table 2 shows the quality assessment results.…”

“…A systematic literature review on the measurement of superoxide dismutase (SOD) activity in patients with gastric cancer identified ten controlled clinical studies that included 1,094 subjects, 495 patients with gastric cancer and 599 healthy individuals [ 13 – 22 ]. Six studies measured SOD in serum, three studies measured SOD in erythrocytes, and one study measured SOD on whole blood.…”