This comprehensive meta-analysis explores the extensive range of traditional medicinal practices in Assam, India. It uncovers the profound ethnobotanical knowledge that is ingrained within the indigenous tribes of Dimasa, Barman, Sonowal, and Bodo. Through a meticulous examination of a dataset comprising 750 entries from 12 tribes and encompassing 354 distinct botanical species, this study sheds light on the diverse and specific plant-based remedies that have been passed down through generations. By utilizing advanced statistical and computational methodologies, such as cluster analysis and Chi-Square tests, our research uncovers significant associations between plant usage and the physiological systems they treat. This underscores the extensive medicinal knowledge held by the Bodo tribe. The results highlight the possibility of combining this conventional knowledge with contemporary medical care, promoting the protection of cultural heritage and biodiversity. This investigation not only adds to the worldwide comprehension of ethnomedicine but also underscores the pressing requirement for recording, sustainable collection methods, and scientific confirmation of traditional treatments. By adopting a comprehensive approach that connects traditional understanding and scientific investigation, this research establishes fresh prospects for medical advancement, sustainability, and the safeguarding of intangible cultural heritage.