In this paper, the adaptive time reversal (ATR) technique combined with a single channel decision feed back equalizer (DFE) is applied to an at-sea experiment of the underwater acoustic multiple-input/multiple-output (MIMO) communication utilizing the signal bandwidth of 4.5-8.5 kHz over the distance of 12.5 km. We focus on the demonstration of the effectiveness of the ATR-DFE schemes for MIMO communication in real sea environment, and how much the communication data rate could be achieved, with changing the signal parameters. It is also investigated the influences of the time variation of the channel to the communication performances. Finally, the relationship between the effective data rate and the bit error rate (BER) is evaluated. As a result, effective data rate of tens of kbps is achieved with practical BER by ATR-DFE, and it can be said that the performance would be a remarkable considering the distance of the communication.