Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment

Kim, Jinseok; Park, Jungyul; Na, Kyounghwan; Yang, Sungwook; Baek, Jeongeun; Yoon, Eui-Sung; Choi, Sung-Sik; Lee, Sang-Ho; Chun, Kukjin; Park, Jong-Oh; Park, Sukho

doi:10.1016/j.jbiomech.2008.05.036

Cited by 47 publications

(37 citation statements)

References 18 publications

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“…To further investigate the influence of topography on functionality of cardiomyocytes, contractile forces generated by cardiomyocytes cultured on flat and a 10 µm-wide microcantilevers was measured in another study. 98 Cells cultured on microcantilevers showed anisotropic actin organisation and had 65-85% higher contractile forces compared with flat surfaces. In addition, it was shown that expression of junctional markers such as N-cadherin and connexion-43 upregulated in presence of certain arrangements of micropillars, further suggesting enhancement of cardiomyocytes functionality by surface topography.…”

Section: Differentiationmentioning

confidence: 99%

Surface engineering of synthetic polymer materials for tissue engineering and regenerative medicine applications

2014

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When using polymer materials as scaffolds for tissue engineering or regenerative medicine applications the initial, and often lasting, interaction between cells and the material are via surfaces. Surface engineering is an important strategy in materials fabrication to control and tailor cell interactions whilst preserving desirable bulk materials properties. Surface engineering methods have been described that can strongly influence cell adhesion, migration, proliferation, differentiation and functionality. This review aims to categorise the current strategies for modifying surface chemistry and/or topography in terms of the resultant change in cell behaviour.

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Section: Differentiationmentioning

confidence: 99%

Surface engineering of synthetic polymer materials for tissue engineering and regenerative medicine applications

2014

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“…(10). Images of the flat and grooved microcantilevers: (a) Fabricated PDMS flat and grooved microcantilever, (b) ESEM image of the hybrid organicinorganic flat and grooved microcantilevers, (c) and (d) still images from video recordings of the vertical motion of the 200 1000 μm hybrid biopolymer microcantilevers [72].…”

Section: Discussionmentioning

confidence: 99%

“…The integration of muscle cells on grooved structured cantilever were found to generate more bending than on the flat surfaces as shown in Fig. (10) [70][71][72]. The forces generated by the living cells on the microcantilever structures have been harnessed to create cell-powered mechanical motors [73].…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

Li¹,

Shu

2011

COC

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Microcantilever based biosensors have attracted much attention as a label-free, real-time and highly sensitive approach to the detection of biomolecules. When specific biomolecular interactions occur between a receptor immobilized on one side of a cantilever and a target analyte in solution, a mechanical bending of the cantilever results due to a change in surface stress, thereby translating biochemical interactions into a concentration-dependent nanomechanical response of the microcantilevers. In this review, we present a general overview of the operation principles, fabrication and preparation of microcantilever biosensors and discuss their uses for a wide range of biosensing applications. The focus of the review is given to probing the biomolecular interactions at the solid-liquid interface by measuring the cantilever bending. In addition to the label-free detection of DNA, protein and cells, the emphasis is also made to discuss about the new development of microcantilever biosensors for label-free probing nanoscale conformational changes of DNA, protein and polymers, and real-time detection of nanomechanical forces from living cells. Finally, the outlook for future development work and challenges is also discussed.

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“…38 Microtissues composed of sufficient numbers of cells to mimic the tissue, while remaining small enough for incorporation into microscale devices, are more appropriate for mimicking heart function. Microtissue systems for measuring cardiomyocyte force generation have been created using elastomeric PDMS in the form of cantilevers, [39][40][41] lms attached at one end, 17,18 lms mounted on posts, 42,43 and using hydrogel materials for cantilevers 44 and posts. 45 The measurement of the electrical activity of cardiomyocyte cultures is commonly performed using microelectrode arrays, in which an array of microscale electrodes records voltage changes due to depolarisation and repolarisation of the cell membranes during activity at various positions on the culture surface.…”

Section: Heart/cardiac Tissuementioning

confidence: 99%

‘Body-on-a-Chip’ Technology and Supporting Microfluidics

Smith¹,

Long²,

McAleer³

et al. 2014

Human-Based Systems for Translational Research

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In order to effectively streamline current drug development protocols, there is a need to generate high information content preclinical screens capable of generating data with a predictive power in relation to the activity of novel therapeutics in humans. Given the poor predictive power of animal models, and the lack of complexity and interconnectivity of standard in vitro culture methodologies, many investigators are now moving toward the development of physiologically and functionally accurate culture platforms composed of human cells to investigate cellular responses to drug compounds in high-throughput preclinical studies. The generation of complex, multi-organ in vitro platforms, built to recapitulate physiological dimensions, flow rates and shear stresses, is being investigated as the logical extension of this drive. Production and application of a biologically accurate multi-organ platform, or ‘body-on-a-chip’, would facilitate the correct modelling of the dynamic and interconnected state of living systems for high-throughput drug studies as well as basic and applied biomolecular research. This chapter will discuss current technologies aimed at producing ‘body-on-a-chip’ models, as well as highlighting recent advances and important challenges still to be met in the development of biomimetic single-organ systems for drug development purposes.

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Quantitative evaluation of cardiomyocyte contractility in a 3D microenvironment

Cited by 47 publications

References 18 publications

Surface engineering of synthetic polymer materials for tissue engineering and regenerative medicine applications

Surface engineering of synthetic polymer materials for tissue engineering and regenerative medicine applications

Microcantilever Biosensors: Probing Biomolecular Interactions at the Nanoscale

‘Body-on-a-Chip’ Technology and Supporting Microfluidics

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