Quantitative evaluation of ischemia–Reperfusion injury by optical coherence tomography in the rat retina

Sho, Kenichiro; Takahashi, Kanji; Fukuchi, Toshio; Matsumura, Miyo

doi:10.1007/s10384-004-0150-3

Cited by 20 publications

(14 citation statements)

References 18 publications

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“…The reperfusion injury in a case of CRAO is being reported for the first time in human and corroborates well with the study on a rat model where similar findings were observed on day 4 and 7, respectively 5. Timeline of reperfusion injury in CRAO gives us a novel insight into the critical period for treatment.…”

Section: Discussionsupporting

confidence: 88%

Ischaemia-reperfusion injury in central retinal artery occlusion

et al. 2013

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SUMMARYA 53-year-old man presented with sudden painless diminution of vision in his right eye for 3 days. His fundus examination showed diffuse whitening of the retina with a cherry red spot at the fovea with cilioretinal artery sparing. On fluorescein angiography delayed arteriovenous transit was observed. Three-dimensional spectral domain optical coherence tomography was used to assess retinal nerve fibre layer thickness and average macular central subfield thickness on days 3, 7, 30 and 90. Marked retinal oedema due to ischaemia was observed on day 3 of occurrence of central retinal artery occlusion. On day 7, significant decrease in retinal nerve fibre thickness and macular thickness was noted suggestive of acute reperfusion injury. Retinal nerve fibre layer thickness and macular thickness returned to near normal on day 30 due to restoration of blood supply with wash out of stress mediators. Retinal atrophy was observed on day 90. BACKGROUND

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Section: Discussionsupporting

confidence: 88%

Ischaemia-reperfusion injury in central retinal artery occlusion

et al. 2013

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“…MEMRI-derived thickness of the entire neural retina (≈235 μm), excluding the choroidal vascular layer, is in good agreement with the total thickness of the rat neural retina reported using histology (24,25), ultrasound (26), and optical coherence tomography (27,28). Importantly, thicknesses of individual layers determined by our histology are also in general agreement with those reported by histology (24) and ultrasound (26).…”

Section: Discussionsupporting

confidence: 81%

Manganese‐enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas

Nair

Pardue

Kim

et al. 2011

Magnetic Resonance Imaging

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Purpose To use manganese-enhanced magnetic resonance imaging (MEMRI) at 25 × 25 × 800 μm3 to image different retinal and vascular layers in the rat retinas. Materials and Methods Manganese-chloride was injected intraocularly in normal (n = 5) and Royal College of Surgeons (RCS, an model of photoreceptor degeneration) (n = 5) rats at postnatal day 90. MEMRI at 4.7 T was performed 24 hours later. MRI was repeated following intravenous Gd-DTPA in the same animals to highlight the vasculatures. Layer assignment and thickness were compared to histology. Results MEMRI 24 hours after intravitreal manganese-chloride injection revealed seven bands of alternating hyper- and hypointensities, corresponding histologically to the ganglion cell layer, inner plexiform layer, inner nuclear layer, outer plexiform layer, outer nuclear layer, photoreceptor-segment layer, and choroidal vascular layer. Intravenous Gd-DTPA—which does not cross the blood–retinal barrier and the retinal pigment epithelium—further enhanced the two layers bounding the retina, corresponding to the retinal and choroidal vascular layers, but not the avascular outer nuclear layer and the photoreceptor-segment layer. MEMRI of the RCS retinas revealed the loss of the outer plexiform layer, outer nuclear layer, and photoreceptor-segment layer. Histological analysis corroborated the MRI laminar assignments and thicknesses. Conclusion Lamina-specific retinal structures neurodegenerative changes to structure in retinal diseases can be detected using MEMRI.

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“…This technique is infrequently used in rodent studies due to the technical challenges presented by small rodent eyes. However, when successfully applied in animal models, OCT is an extremely valuable tool as it measures retinal thickness (Fukuchi et al, 2001; Horio et al, 2001; Sho et al, 2005), the critical consequence of excessive vascular permeability and a frequently used endpoint in clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Topical administration of a multi‐targeted kinase inhibitor suppresses choroidal neovascularization and retinal edema

Doukas¹,

Mahesh²,

Umeda

et al. 2008

Journal Cellular Physiology

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Age-related macular degeneration, diabetic retinopathy, and retinal vein occlusions are complicated by neovascularization and macular edema. Multi-targeted kinase inhibitors that inhibit select growth factor receptor tyrosine kinases and/or components of their down-stream signaling cascades (such as Src kinases) are rationale treatment strategies for these disease processes. We describe the discovery and characterization of two such agents. TG100572, which inhibits Src kinases and selected receptor tyrosine kinases, induced apoptosis of proliferating endothelial cells in vitro. Systemic delivery of TG100572 in a murine model of laser-induced choroidal neovascularization (CNV) caused significant suppression of CNV, but with an associated weight loss suggestive of systemic toxicity. To minimize systemic exposure, topical delivery of TG100572 to the cornea was explored, and while substantial levels of TG100572 were achieved in the retina and choroid, superior exposure levels were achieved using TG100801, an inactive prodrug that generates TG100572 by de-esterification. Neither TG100801 nor TG100572 were detectable in plasma following topical delivery of TG100801, and adverse safety signals (such as weight loss) were not observed even with prolonged dosing schedules. Topical TG100801 significantly suppressed laser-induced CNV in mice, and reduced fluorescein leakage from the vasculature and retinal thickening measured by optical coherence tomography in a rat model or retinal vein occlusion. These data suggest that TG100801 may provide a new topically applied treatment approach for ocular neovascularization and retinal edema.

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Quantitative evaluation of ischemia–Reperfusion injury by optical coherence tomography in the rat retina

Abstract: OCT can detect retinal changes quantitatively after ischemia-reperfusion injury, and the retinal thicknesses obtained from OCT images are probably a better measure of the true retinal thickness than those measured on histological sections.

Cited by 20 publications

References 18 publications

Ischaemia-reperfusion injury in central retinal artery occlusion

Ischaemia-reperfusion injury in central retinal artery occlusion

Manganese‐enhanced MRI reveals multiple cellular and vascular layers in normal and degenerated retinas

Topical administration of a multi‐targeted kinase inhibitor suppresses choroidal neovascularization and retinal edema

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