Quantitative evaluation of sphingomyelin and glucosylceramide using matrix-assisted laser desorption ionization time-of-flight mass spectrometry with sphingosylphosphorylcholine as an internal standard

Fujiwaki, Takehisa; Tasaka, Masaru; Yamaguchi, Seiji

doi:10.1016/j.jchromb.2008.05.013

Cited by 23 publications

(20 citation statements)

References 19 publications

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“…The resulting lyso-sulfatides were extracted with a C18 MicroTip and analyzed by MALDI-TOF from CHCA allowing quantitation of serum sulfatides from one hundred-to 50-mL serum specimens within 1 working day. A quantitative method has been described for ceramide monohexoside and sphingomyelin using sphingosylphosphorylcholine as the internal standard (Fujiwaki, Tasaka, & Yamaguchi, 2008) and DHB as the MALDI matrix. The calibration curve for ceramide monohexoside was linear over the range 5-150 ng and the method was used to measure accumulations of the glycolipids in liver and spleen samples from patients with Gaucher disease.…”

Section: Other Methodsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Harvey

2011

Mass Spectrometry Reviews

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This review is the fifth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2008. The first section of the review covers fundamental studies, fragmentation of carbohydrate ions, use of derivatives and new software developments for analysis of carbohydrate spectra. Among newer areas of method development are glycan arrays, MALDI imaging and the use of ion mobility spectrometry. The second section of the review discusses applications of MALDI MS to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, biopharmaceuticals, glycated proteins, glycolipids, glycosides and various other natural products. There is a short section on the use of MALDI mass spectrometry for the study of enzymes involved in glycan processing and a section on the use of MALDI MS to monitor products of the chemical synthesis of carbohydrates with emphasis on carbohydrate-protein complexes and glycodendrimers. Corresponding analyses by electrospray ionization now appear to outnumber those performed by MALDI and the amount of literature makes a comprehensive review on this technique impractical. However, most of the work relating to sample preparation and glycan synthesis is equally relevant to electrospray and, consequently, those proposing analyses by electrospray should also find material in this review of interest.

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Section: Other Methodsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Harvey

2011

Mass Spectrometry Reviews

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“…Sphingosylphosphorylcholine was used as the internal standard for quantification of sphingomyelin and ceramide monohexoside (CMH). The authors report that accumulated sphingomyelin and CMH in small amounts of tissues from sphingolipidosis patients can be quantified for diagnostic purposes and also for biochemical pathophysiology evaluation [58].…”

Section: Lipidsmentioning

confidence: 99%

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Duncan¹,

Röder²,

Hunsucker³

2008

Briefings in Functional Genomics and Proteomics

197

162

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This review summarizes the essential characteristics of matrix-assisted laser desorption/ionization (MALDI) timeof-flight mass spectrometry (TOF MS), especially as they relate to its applications in quantitative analysis. Approaches to quantification by MALDI-TOF MS are presented and published applications are critically reviewed.Keywords: quantification; quantitative analysis; MALDI; mass spectrometry; biomarkers OVERVIEWSince its inception in 1987 [1], matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF MS) has been applied for the analysis of a wide range of biomolecules. Initial applications were almost exclusively for the qualitative analysis of biopolymers because MALDI-TOF MS provided a fast and accurate approach to molecular mass and purity information: Is my material the right mass and is it free from contaminants? Because of the speed of analysis, ease of use, relative low equipment cost, ease of data interpretation and limited potential for cross-contamination between samples/users, MALDI-TOF MS systems were considered walk-up instruments where investigators run their own samples and determine, within minutes, whether they were on the right track with their work.There were, however, two specific areas of application where MALDI-TOF MS was initially viewed as impractical: i.e. the analysis of low mass analytes and quantitative applications. Low mass analysis is complicated by the vast molar excess of the matrix that can swamp any analyte-specific signal in the low m/z region of the spectrum. Quantitative analysis was viewed as implausible because crystallization does not yield a uniform distribution of the analyte and matrix across the target surface and this gives rise to regions where the analyte signal is especially intense relative to other locations on the target surface. MALDI MS was therefore viewed as inherently irreproducible because, for a given amount of analyte loaded onto the target, the measured ion intensity varies.Subsequently, it has been shown that both these perceived limitations can be overcome and quantification can be routine, both for low and high mass analytes. Now, an extensive list of published applications includes quantification of amino acids, lipids, natural products, drugs, polymers, herbicides, metabolites, toxins, oligonucleotides, carbohydrates, peptides and proteins. (Selected applications from these areas are discussed at the end of this review.) Here, we focus on the quantitative applications of MALDI and illustrate applications relating to both low and high-molecular mass analytes. We also discuss the strategies for applying MALDI to relative and absolute quantification. While MALDI is most commonly combined with TOF analysers, other analyser options are possible and applications employing these are also presented. Our aim is not to provide an exhaustive catalogue of published applications, but to discuss the general principles and to illustrate the

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“…MALDI-TOF/MS was also used to characterize lipid biomarkers extracted by organic solvent from human serum. After the major classes of lipoproteins were isolated from serum samples by ultracentrifugation, the lipids were extracted from lipoproteins by Folch's solution (chloroform/ methanol 2:1, v/v) and analyzed by MALDI-TOF/MS to determine lipid biomarkers such as sphingolipids in liver, spleen, and cardiac valve samples for Niemann-Pick disease, Gaucher disease, and Fabry disease, respectively [230][231][232]. Sphingosylphosphorylcholine was used as the internal standard for quantification of sphingomyelin and ceramidemonohexoside.…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

“…Sphingosylphosphorylcholine was used as the internal standard for quantification of sphingomyelin and ceramidemonohexoside. Accumulated sphingomyelin and ceramidemonohexoside in the tissues collected from sphingolipidosis patients were quantified for clinical diagnosis and for biochemical and pathophysiological evaluation [230,231]. Ceramide trihexose was detected at high concentrations in samples with Fabry disease [232].…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

Biomarker Characterization by MALDI–TOF/MS

Cho¹,

et al. 2015

Advances in Clinical Chemistry

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Quantitative evaluation of sphingomyelin and glucosylceramide using matrix-assisted laser desorption ionization time-of-flight mass spectrometry with sphingosylphosphorylcholine as an internal standard

Cited by 23 publications

References 19 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Biomarker Characterization by MALDI–TOF/MS

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