Quantitative Evaluation of the Factors Affecting the Process of Fibroblast-Mediated Collagen Gel Contraction by Separating the Process into Three Phases

Nishiyama, Toshio; Tominaga, Naoki; Nakajima, Keisuke; Hayashi, Toshihiko

doi:10.1016/s0174-173x(88)80045-1

Cited by 81 publications

(57 citation statements)

References 23 publications

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“…This collagen concentration-dependent effect on the transmission of mechanical signals may affect, for example, intercellular mechanical communication, which is fundamental to wound healing [42], tissue development [43] and cancer cell metastasis [44]. The collagen concentration-dependent remodelling of networks has been reported earlier [45], and it has been noted that the extent of network deformation decreases with higher collagen concentrations [46,47]. In contrast to these reports, our results show a much earlier decay in the rate of network deformation by adherent cells on dense networks.…”

Section: Discussionmentioning

confidence: 95%

Inelastic behaviour of collagen networks in cell–matrix interactions and mechanosensation

Mohammadi

Arora

Simmons

et al. 2015

J. R. Soc. Interface.

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The mechanical properties of extracellular matrix proteins strongly influence cell-induced tension in the matrix, which in turn influences cell function. Despite progress on the impact of elastic behaviour of matrix proteins on cell-matrix interactions, little is known about the influence of inelastic behaviour, especially at the large and slow deformations that characterize cell-induced matrix remodelling. We found that collagen matrices exhibit deformation rate-dependent behaviour, which leads to a transition from pronounced elastic behaviour at fast deformations to substantially inelastic behaviour at slow deformations (1 mm min 21, similar to cell-mediated deformation). With slow deformations, the inelastic behaviour of floating gels was sensitive to collagen concentration, whereas attached gels exhibited similar inelastic behaviour independent of collagen concentration. The presence of an underlying rigid support had a similar effect on cell-matrix interactions: cell-induced deformation and remodelling were similar on 1 or 3 mg ml 21 attached collagen gels while deformations were two-to fourfold smaller in floating gels of high compared with low collagen concentration. In cross-linked collagen matrices, which did not exhibit inelastic behaviour, cells did not respond to the presence of the underlying rigid foundation. These data indicate that at the slow rates of collagen compaction generated by fibroblasts, the inelastic responses of collagen gels, which are influenced by collagen concentration and the presence of an underlying rigid foundation, are important determinants of cell-matrix interactions and mechanosensation.

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Section: Discussionmentioning

confidence: 95%

Inelastic behaviour of collagen networks in cell–matrix interactions and mechanosensation

Mohammadi

Arora

Simmons

et al. 2015

J. R. Soc. Interface.

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“…The cellseeded collagen gel was then returned for incubation overnight in the C 0 2 incubator. The contracted cell-collagen mix was used as the implant for surgery the following day [2,32,34].…”

Section: Preparufion Of Bmp-2 Cwgineercd M S Cmentioning

confidence: 99%

Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats

Tsuchida

Hashimoto

Crawford

et al. 2003

Journal Orthopaedic Research

179

121

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Bone marrow derived mesenchymal stem cells (MSC) have been shown to be progenitor cells for mesenchymal tissues. These cells may also provide a potential therapy for bone repair. Our previous studies showed that MSC engineered with the gene for bone morphogenetic protein 2 (BMP-2), a growth factor for bone cells, were capable of differentiating into osteoblast lineage and inducing autologous bone formation in several animal models. Culturing individual MSC for autologous implantation, however, remains problematic. The number of human MSC with osteogenic potential decreases with age, and, in certain diseases, the patient's marrow may be damaged or the healthy cells reduced in number. In this study, we used rats with a femoral segmental defect to investigate whether allogeneic BMP-2 engineered MSC would facilitate bone healing. We show that BMP-2 engineered allogeneic MSC can repair critical bone defects to the same degree as rats treated with BMP-2 engineered autologous MSC, if the allogeneic group receives short-term treatment with immunosuppressant FK506. We also show that allogeneic gene transferred MSC are directly involved in bone repair, in addition to acting as gene deliverers.

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“…More important size changes occur when the tissue shrinks. For instance it is known for a long time that widely used collagen type I gels, which are often used in tissue engineering studies, compact significantly after a few days culture [69,70]. Updating piston height is required to continue loading such construct, and these scaffolds are not suitable for use in confined compression setups as the construct will not stay in contact with the container.…”

Section: Discussionmentioning

confidence: 99%

Review on Patents for Mechanical Stimulation of Articular Cartilage Tissue Engineering

Donkelaar¹,

Schulz

2008

BIOMENG

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Abstract:To repair articular cartilage defects in osteoarthritic patients with three-dimensional tissue engineered chondrocyte grafts, requires the formation of new cartilage with sufficient mechanical properties. The premise is that mechanical stimulation during the culturing process is necessary to reach this aim. Therefore, mechanical stimulation systems have been integrated in aseptic bioreactors for in vitro cultivation of tissue engineered cartilage. These vary from simple unconfined compression systems to advanced bioreactors in which deformation and loading are fully controlled. Fluid handling in these devices is another decisive parameter for the success of cartilage tissue engineering.Over the last decades bioreactor developments have resulted in the filing of many patents. The aim of this paper is to review these patents, categorize them according to their possibilities for mechanical stimulation and fluid handling systems and finally to discuss them in the context of the demands of a functional tissue engineered cartilage from a mechanical perspective.

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Quantitative Evaluation of the Factors Affecting the Process of Fibroblast-Mediated Collagen Gel Contraction by Separating the Process into Three Phases

Cited by 81 publications

References 23 publications

Inelastic behaviour of collagen networks in cell–matrix interactions and mechanosensation

Inelastic behaviour of collagen networks in cell–matrix interactions and mechanosensation

Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats

Review on Patents for Mechanical Stimulation of Articular Cartilage Tissue Engineering

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