Quantitative gas—liquid chromatographic determination of medroxyprogesterone acetate in human plasma

Rossi, Ennio C.; Pascale, A. De; Negrini, P.; Frigerio, A.; Castegnaro, E

doi:10.1016/0021-9673(75)85073-4

Cited by 14 publications

(6 citation statements)

References 7 publications

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“…Several RIA methods (Cornette et al 1971;Hiroi et al 1975;Royer et al 1974;Shrimanker et al 1978;Sletholt 1981;Smith et al 1979;Victor & Johansson 1976), gas liquid chromatography (GLC) [Kaiser et al 1974;Pannuti et al 1982;Rossi et al 1979], HPLC (Milano et al 1982), enzymatic detection (Adlercreutz & Harkonen 1980) and GC-MS techniques (Adlercreutz et al 1974;Phillippou & Frith 1980) have been published.…”

Section: Drug Measurementmentioning

confidence: 98%

Clinical Pharmacokinetics of Endocrine Agents Used in Advanced Breast Cancer

Lønning

Lien

Lundgren

et al. 1992

Clinical Pharmacokinetics

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Endocrine therapy is important in the treatment of advanced breast cancer. The prototype antiestrogen tamoxifen and the prototype aromatase inhibitor aminoglutethimide have been in clinical use for more than 2 decades, as have synthetic progestin derivatives. Currently, several novel antiestrogens and aromatase inhibitors are used to treat breast cancer. This paper reviews the present knowledge of the clinical pharmacokinetics of these drugs. Drug monitoring in plasma and other body fluids has been improved over recent years by the introduction of sensitive and specific high performance liquid chromatography and gas chromatography-mass spectrometry methods. However, we still lack information on such basic pharmacokinetic parameters as the bioavailability of several of these drugs. It is important to study not only plasma but also tissue drug concentrations.

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Section: Drug Measurementmentioning

confidence: 98%

Clinical Pharmacokinetics of Endocrine Agents Used in Advanced Breast Cancer

Lønning

Lien

Lundgren

et al. 1992

Clinical Pharmacokinetics

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“…Numerous recent researches for this analysis have been reported concerning the method sensitivity and selectivity in association with various instruments including GC, HPLC and others [6][7][8][9][10][11][12][13][14][15]. However, each of which had their advantages and disadvantages.…”

Section: Determination Of Mpa In Hormonal Contraceptive Drugsmentioning

confidence: 99%

“…However, this procedure could not be applied for MPA analysis in human plasma due to its high matrix interference. Attempts have been made to optimize the methods for MPA analysis using small amounts of plasma samples [7,8]. Quantitative analysis of MPA in plasma by HPLC with ultraviolet (UV) detection was also carried out, but did not give satisfactory detection limit [9,10].…”

Section: Determination Of Mpa In Hormonal Contraceptive Drugsmentioning

confidence: 99%

Application of HPLC Analysis of Medroxyprogesterone Acetate in Human Plasma

Chanthai¹,

Tessiri²

2012

Chromatography - The Most Versatile Method of Chemical Analysis

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“…Recently, several methods have been demonstrated for the determination of MPA in plasma and serum using GC-ECD [5,6] or GC-MS [7,8]. The former technique is limited in sensitivity and selectivity, due to interference from endogenous cholesterol.…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive analysis of medroxyprogesterone acetate in human serum by gas chromatography-mass spectrometry

Choi¹,

Kim

Kim³

et al. 2001

Chromatographia

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SummaryMedroxyprogesterone acetate (MPA) is one of the major drugs used in endocrine therapy for advanced or recurrent breast cancer. The present study describes an accurate and reproducible gas chromatographic-mass spectrometric (GC-MS) method to measure MPA in serum.After being extracted bya solid-phase cartridge using an Oasis HLB copolymer, the residues were derivatized with a heptafluorobuf,/ric anhydride and then analyzed by GC-MS.We validated the method by analyzing the serum samples spiked with pure MPA, and obtained a linear correlation (0.9977) and precision with a coefficient of variation ranging from 1.8 to 7.3. We found that the GC-MS method was linear over the range of 0.2 ng mL 1 to 500 ng mL 1 using a sample volume of 1 mL. The analytical recoverywas 93.7%, and the limit of detection was 0.2 ng mL 1.The oral administration of MPA to men and its subsequent detection in serum showed that the GC-MS method described is sensitive, specific, and suitable for the determination of MPA by bench-top GC-MS.

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Quantitative gas—liquid chromatographic determination of medroxyprogesterone acetate in human plasma

Cited by 14 publications

References 7 publications

Clinical Pharmacokinetics of Endocrine Agents Used in Advanced Breast Cancer

Clinical Pharmacokinetics of Endocrine Agents Used in Advanced Breast Cancer

Application of HPLC Analysis of Medroxyprogesterone Acetate in Human Plasma

Rapid and sensitive analysis of medroxyprogesterone acetate in human serum by gas chromatography-mass spectrometry

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