Quantitative Gene Expression Analysis by cDNA Microarray During Liver Regeneration After Partial Hepatectomy in Rats

Lai, Hong‐Shiee; Chen, Yun; Lin, Wen-Hsi; Chen, Chiung-Nien; Wu, Huey-Dong; Chang, Chee‐Jen; Lee, Po‐Huang; Chang, King‐Jen; Chen, Wei-Jao

doi:10.1007/s00595-004-2962-7

Cited by 18 publications

(9 citation statements)

References 26 publications

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“…In the rat, hepatocytes start entering S phase after 12 h and peak DNA synthesis occurs by 24 h after PHx, followed ϳ24 h later by proliferation of nonparenchymal cells (38). During the initial 24 h following PHx an extensive program of gene expression changes is activated, as previously demonstrated by our group and others (9,19,23) (R. Vadigepalli, unpublished data).…”

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confidence: 52%

Chronic ethanol feeding enhances miR-21 induction during liver regeneration while inhibiting proliferation in rats

Dippold

Vadigepalli

Gonye

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Liver regeneration is an important repair response to liver injury. Chronic ethanol consumption inhibits and delays liver regeneration in experimental animals. We studied the effects of chronic ethanol treatment on messenger RNA (mRNA) and microRNA (miRNA) expression profiles during the first 24 h after two-thirds partial hepatectomy (PHx) and found an increase in hepatic miR-21 expression in both ethanol-fed and pair-fed control rats after PHx. We demonstrate that the increase of miR-21 expression during liver regeneration is more robust in ethanol-fed rats. Peak miR-21 expression occurs at 24 h after PHx in both ethanol-fed and control rats, corresponding to the peak of hepatocyte S phase in control rats, but not in ethanol-exposed livers in which cell cycle is delayed. The induction of miR-21 24 h after PHx in control rats is not greater than the increase in expression of miR-21 due to sham surgery. However, in the ethanol-fed rat, miR-21 is induced to a greater extent by PHx than by sham surgery. To elucidate the implications of increased miR-21 expression during liver regeneration, we employed unbiased global target analysis using gene expression data compiled by our group. Our analyses suggest that miR-21 may play a greater role in regulating gene expression during regeneration in the ethanol-fed rat than in the control rat. Our analysis of potential targets of miR-21 suggests that miR-21 affects a broad range of target processes and may have a widespread regulatory role under conditions of suppressed liver regeneration in ethanol-treated animals.

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confidence: 52%

Chronic ethanol feeding enhances miR-21 induction during liver regeneration while inhibiting proliferation in rats

Dippold

Vadigepalli

Gonye

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…4 In our previous study of liver regeneration after PH, we showed that the variations in gene expression could be classified into 72 different patterns via a mass survey of gene expression by cDNA microarray. 5 We also discovered that the isolated hepatocytes have a stem cell-like strong proliferation ability and can repopulate extensively in the host liver after suitable preconditioning treatment of recipients in liver transplantation. 6 Liver regeneration constitutes a conditional cell renewal system that may proceed under well-defined conditions such as PH.…”

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confidence: 98%

Lipocalin-2 gene expression during liver regeneration after partial hepatectomy in rats

Lai

et al. 2013

International Journal of Surgery

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“…In rats and mice, partial hepatectomy triggers a sequence of regeneration events from the first 30 minutes to 7 days after the procedure. A large number of genes with either new or increased expression in the regenerating liver have been identified and categorized into several phases of activity, including immediate-early, delayed, cell cycle, and DNA replication and mitosis genes [3], [4].…”

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confidence: 99%

Interleukin-6 Mediates Angiotensinogen Gene Expression during Liver Regeneration

et al. 2013

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BackgroundAngiotensinogen is the precursor of angiotensin II, which is associated with ischemia-reperfusion injury. Angiotensin II reduces liver regeneration after hepatectomy and causes dysfunction and failure of reduced-size liver transplants. However, the regulation of angiotensinogen during liver regeneration is still unclear.AimsTo investigate the regulation of angiotensinogen during liver regeneration for preventing angiotensin II-related ischemia-reperfusion injury during liver regeneration.MethodsA mouse in vitro partial hepatectomy animal model was used to evaluate the expression of interleukin-6 (IL-6) and angiotensinogen during liver regeneration. Serum IL-6 and angiotensinogen were detected by enzyme immunoassay (EIA). Angiotensinogen mRNA was detected by RT-PCR. Tissue levels of angiotensinogen protein were detected by Western blot analysis. Primary cultures of mouse hepatocytes were used to investigate IL-6-induced angiotensinogen. Chemical inhibitors were used to perturb signal transduction pathways. Synthetic double-stranded oligodeoxynucleotides (ODNs) were used as ‘decoy’ cis-elements to investigate transcription. Ki 67 staining and quantification were used to verify liver regeneration.ResultsIn the in vivo model, the levels of serum IL-6 and angiotensinogen correlated. In the in vitro model, IL-6 transcriptionally regulated angiotensinogen expression. Additionally, IL-6 mediated angiotensinogen expression through the Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) and JAK/p38 signaling. Decoy ODN analyses revealed that STAT3 and nuclear factor-kB (NF-kB) also played critical roles in the transcriptional regulation of angiotensinogen by IL-6. IL-6-mediated signaling, JAK2, STAT3 and p38 inhibitors reduced angiotensinogen expression in the partially hepatectomized mice.ConclusionDuring liver regeneration, IL-6-enhanced angiotensinogen expression is dependent on the JAK/STAT3 and JAK/p38/NF-kB signaling pathways. Interruption of the molecular mechanisms of angiotensinogen regulation may be applied as the basis of therapeutic strategies for preventing angiotensin II-related ischemia-reperfusion injury during liver regeneration.

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Quantitative Gene Expression Analysis by cDNA Microarray During Liver Regeneration After Partial Hepatectomy in Rats

Abstract: The quantitative gene expression profiles have important implications, warranting further investigation of the genetic mechanisms involved in the process of liver regeneration.

Cited by 18 publications

References 26 publications

Chronic ethanol feeding enhances miR-21 induction during liver regeneration while inhibiting proliferation in rats

Chronic ethanol feeding enhances miR-21 induction during liver regeneration while inhibiting proliferation in rats

Lipocalin-2 gene expression during liver regeneration after partial hepatectomy in rats

Interleukin-6 Mediates Angiotensinogen Gene Expression during Liver Regeneration

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