Quantitative gene expression analysis of some sodium ion transporters under salinity stress in Aeluropus littoralis

Abstract

Plant sodium transporters activity is one of the most important salt tolerance mechanisms to keep normal status of cytosolic sodium content. In the present study, expression pattern of genes encoding Na(+)/H(+) antiporters in the plasma membrane (SOS1 gene), vacuolar membrane (NHX1 gene) and H(+)-ATPase pump (VHA gene) in Aeluropus littoralis under different treatments of NaCl was measured by the semi-quantitative RT-PCR method. Our results indicated that root and shoot sodium contents were increased along with increasing salinity pressure. In response to 200 and 400 mM NaCl, mRNA level of SOS1 and NHX1 was increased in the shoot and root tissues, while VHA transcripts were increased only under 400 mM of NaCl. Transcripts of VHA-c and NHX1 in root were higher than shoot in all treatments. In general, our results indicated that transcriptional level of SOS1, and NHX1 genes induced in parallel with VHA expression may be involved in controlling cytosolic Na(+) concentration in A. littoralis.

Physico-biochemical and molecular responses of Acacia auriculiformis to salinity stress

“…All these events, in turn, facilitated ROS mediated cross-linking in cell wall polymeric components and support our observation of phloroglucinol staining. In corroboration to our present findings, earlier study documented that strong vascular tissue development is helpful for the seedlings to withstand salt stress in a better manner [43]. The antioxidant response at 400 mM NaCl thus improved the cell wall integrity and membrane permeability that might lead to greater accumulation of Na + in cytosol.…”

Calcium Improves Germination and Growth of Sorghum bicolor Seedlings under Salt Stress

“…The expression of the antiporter genes, SOS1, and vacuolar NHX2 that are responsible for maintaining ion homeostasis [ 71 , 72 ] were measured. SbSOS1 was upregulated under 300 mM NaCl stress in sorghum seedlings ( Figure 7 C).…”

Temporal and spatial changes in ion homeostasis, antioxidant defense and accumulation of flavonoids and glycolipid in a halophyte Sesuvium portulacastrum (L.) L.

“…In the present study, we have shown that the three transporters: NHX3 , v-Type ATPase and SOS1 were upregulated under salt treatment. Salt induced, expression of NHX gene triggers the activity of tonoplast based Na + /H + antiporter [ 71 ], and up regulation of NHX alongwith v-Type ATPase and SOS genes may contribute to maintainthe electrochemical proton gradient across tonoplast [ 26 ]. In our study, we observed induction in expression of the tested transporter genes from 6 to 24hr of salt treatment.…”

“…Salicornia bigelovii [ 24 ] and Lobularia maritime [ 25 ]. Induction of both SOS1 and NHX1 in leaf and root tissues of a perennial halophyte, Aeluropus littoralis was suggested to be effective to control Na + translocation and accumulation in leaf tissues [ 26 ].…”