Quantitative genetic analysis of salicylic acid perception in Arabidopsis

Dobón, Albor; Canet, Juan Vicente; Perales, Lorena; Tornero, Pablo

doi:10.1007/s00425-011-1436-6

Cited by 16 publications

(17 citation statements)

References 63 publications

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“…NPR1 has also been described as essential for RIM, but there are differences between alleles, since npr1 -1 and npr1 -3 have different RIM ([20,25]). npr1 -1 and npr1 -3 have other differences in phenotypes related to MeJA.…”

Section: Resultsmentioning

confidence: 99%

The BLADE-ON-PETIOLE genes of Arabidopsis are essential for resistance induced by methyl jasmonate

Canet

Dobón

Fajmonová³

et al. 2012

BMC Plant Biol

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BackgroundNPR1 is a gene of Arabidopsis thaliana required for the perception of salicylic acid. This perception triggers a defense response and negatively regulates the perception of jasmonates. Surprisingly, the application of methyl jasmonate also induces resistance, and NPR1 is also suspected to be relevant. Since an allelic series of npr1 was recently described, the behavior of these alleles was tested in response to methyl jasmonate.ResultsThe response to methyl jasmonate of different npr1s alleles and NPR1 paralogs null mutants was measured by the growth of a pathogen. We have also tested the subcellular localization of some npr1s, along with the protein-protein interactions that can be measured in yeast. The localization of the protein in npr1 alleles does not affect the response to methyl jasmonate. In fact, NPR1 is not required. The genes that are required in a redundant fashion are the BOPs. The BOPs are paralogs of NPR1, and they physically interact with the TGA family of transcription factors.ConclusionsSome npr1 alleles have a phenotype in this response likely because they are affecting the interaction between BOPs and TGAs, and these two families of proteins are responsible for the resistance induced by methyl jasmonate in wild type plants.

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Section: Resultsmentioning

confidence: 99%

The BLADE-ON-PETIOLE genes of Arabidopsis are essential for resistance induced by methyl jasmonate

Canet

Dobón

Fajmonová³

et al. 2012

BMC Plant Biol

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“…First, we did not see any noticeable phenotype in the plants with the three hypomorphic EMS alleles, besides the response to SA. It is true that the first selection was done with BTH, but in the case of npr1, different alleles diverge in their response to MeJA (Dobón et al, 2011). Second, the phenotypes of nrb4-4 and nrb4-5 plants, although dramatic, did not resemble mutants generally impaired in signaling (e.g., hormones and light).…”

Section: How Specific Is Nrb4?mentioning

confidence: 99%

“…Plants with npr1 alleles differ in their response to MeJAinduced resistance (Dobón et al, 2011). nrb4 plants were wild type in their response to MeJA (see Supplemental Figure 1C online) and also showed a wild-type phenotype on MeJA plates (see Supplemental Figure 1D online) and in growth of Pto(cor-) (Mittal and Davis, 1995;see Supplemental Figure 1E online).…”

Section: Nrb4 Is Required For the Sa Responsementioning

confidence: 99%

Non-Recognition-of-BTH4, an Arabidopsis Mediator Subunit Homolog, Is Necessary for Development and Response to Salicylic Acid

2012

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“…Nevertheless, none of these described genes, loci locate at the identified Qpm3.1 locus, and no gene product was annotated in the region as actin-associated linking with the proposed function of HopW1-1; therefore, additional gene(s) conditions natural variation in Arabidopsis response to HopW1-1. Interestingly, by measuring the growth of DC3000 in planta , Dobon et al have identified four QTLs underpinning natural variation in exogenous SA-induced resistance across Arabidopsis accessions, among which SAQ3 , detected in Col-0xLer RILs, locates closely to Qpm3.1 (Dobón et al, 2011). Further investigation on the possible linkage between these two loci may shed new light on the role of SA-dependent pathways in effector-triggered immunity against plant bacterial pathogens.…”

Section: Discussionmentioning

confidence: 99%

Genetic Interaction between Arabidopsis Qpm3.1 Locus and Bacterial Effector Gene hopW1-1 Underlies Natural Variation in Quantitative Disease Resistance to Pseudomonas Infection

et al. 2017

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Wide quantitative variation in plant disease resistance across Arabidopsis wild populations has been documented and the underlying mechanisms remain largely unknown. To investigate the genetic and molecular basis of this variation, Arabidopsis recombinant inbred lines (RILs) derived from Aa-0 × Col-0 and Gie-0 × Col-0 crosses were constructed and used for inoculation with Pseudomonas syringae pathovars maculicola ES4326 (ES4326) and tomato DC3000 (DC3000). Bacterial growth assays revealed continuous distribution across the large differences between the most and the least susceptible lines in the RILs. Quantitative trait locus (QTL) mapping analyses identified a number of QTLs underpinning the variance in disease resistance, among which Qpm3.1, a major QTL on chromosome III from both Aa-0 and Gie-0 accessions, preferentially restricted the growth of ES4326. A genetic screen for the ES4326 gene selectively leading to bacterial growth inhibition on accession Aa-0 uncovered the effector gene hopW1-1. Further QTL analysis of disease in RILs inoculated with DC3000 carrying hopW1-1 showed that the genetic interaction between Qpm3.1 and hopW1-1 determined Arabidopsis resistance to bacterial infection. These findings illustrate the complexity of Arabidopsis-Pseudomonas interaction and highlight the importance of pathogen effectors in delineating genetic architectures of quantitative variation in plant disease resistance.

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Quantitative genetic analysis of salicylic acid perception in Arabidopsis

Cited by 16 publications

References 63 publications

The BLADE-ON-PETIOLE genes of Arabidopsis are essential for resistance induced by methyl jasmonate

The BLADE-ON-PETIOLE genes of Arabidopsis are essential for resistance induced by methyl jasmonate

Non-Recognition-of-BTH4, an Arabidopsis Mediator Subunit Homolog, Is Necessary for Development and Response to Salicylic Acid

Genetic Interaction between Arabidopsis Qpm3.1 Locus and Bacterial Effector Gene hopW1-1 Underlies Natural Variation in Quantitative Disease Resistance to Pseudomonas Infection

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