Quantitative High-Pressure Liquid Chromatographic Determination of Thimerosal in Pharmaceutical Formulations

Fu, Cherng-Chyi; Sibley, Murray J.

doi:10.1002/jps.2600660536

Cited by 16 publications

(2 citation statements)

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“…Moreover, validity of the methods was established by applying standard addition technique where no interference from excepients was found (Table 1). The results obtained from the suggested methods for analysis of three studied drugs in their pure forms were statistically compared to those obtained by the reported methods [26,31] (using F and student's t-tests) and there was no significant difference among them (Table 2), regarding both accuracy and precision.…”

Section: Methods Applicationmentioning

confidence: 98%

“…Whereas, LC was determined by RP-HPLC [26][27][28][29] and electrochemical [11,13] methods. TM was determined by several methods such as spectrophotometric [16], HPLC [15,18,[30][31][32], and electrochemical [19,32] methods. On the other hand, PE and LC were determined together by HPTLC [7] method while TM and PE was determined together by a spectrophotometric [33] method.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Thiomersal, Lidocaine and Phenylepherine in their Ternary Mixture

Abdelwahab¹

2013

J Chromat Separation Techniq

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This work is concerned with the development and validation of two accurate, precise and specific spectrophotometric and chromatographic methods for analysis of Phenylepherine (PE), Lidocaine (LC) and Thiomersal (TM) in their ternary mixture and in Chromoderm ® solution. The developed spectrophotometric method is the mean centering of ratio spectra (MCR) spectrophotometric method that depends on using the mean centered ratio spectra in two successive steps which eliminates the derivative steps and therefore the signal to noise ratio is enhanced. On the other hand, the developed chromatographic method isocratic RP-HPLC method that depends chromatographic separation on Zorbax C 18 column using a mobile phase consisting phosphate buffer: acetonitrile: triethylamine (40:60:0.1, by volume pH=6 with orthophosphoric acid). The flow rate was 0.6 mL min -1 and the eluent was monitored at 245 nm.Factors affecting the developed methods were studied and optimized. Moreover, they have been validated as per ICH guidelines. The developed methods have been successfully applied for determination of PE, LC and TM in different laboratory mixtures and in solution dosage form. Also, Statistical analysis of the results of the two developed methods with each other using F and student's t tests showed no significant difference.

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Section: Methods Applicationmentioning

confidence: 98%