Quantitative histochemical study of cytochrome oxidase in the dLGN of aging rats

Díaz, F.; Villena, Alicia; Requena, V.; González, Pilar; Peláez, Alberto; Vargas, I. Pérez de

doi:10.1016/0047-6374(96)01777-0

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…These results are consistent with the behaviour observed by other researches regarding different brain regions, especially in rodents, where there is little evidence pointing to relevant neuronal loss in the last stages of life (Curcio and Coleman, 1982; Hinds and McNelly, 1979; Morgan et al, 1987). Nevertheless, in another study also carried out on this nucleus, we detected a decrease in the activity of neuronal cytochrome oxidase between 24 and 28 months which was interpreted as reflecting a decrease in the bioenergetic competence of the neurons of the dLGN in old age (Díaz et al, 1996). It is likely that although the number of neurons does not vary in the Wistar breed from 18 months onwards, certain neuronal functions could be affected upon the arrival of old‐age.…”

Section: Discussionmentioning

confidence: 74%

“…Our investigation was carried out on dLGN from albino rats. The aim was to know the effects of age in this region of the visual system to complete others studies in which we had analysed some others aspects, such as the activity of neuronal cytochrome oxidase (Díaz et al, 1996), the content in nuclear and cytoplasmic RNA in single neuronal cells (Villena et al, 1998), and changes in size and shape of neurons in these stages of life (Villena et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

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Stereological age-related changes in neurons of the rat dorsal lateral geniculate nucleus

Díaz¹,

Villena²,

González³

et al. 1999

Anat. Rec.

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Quantitative methods were used to compare the changes taking place in the volume of the dorsal lateral geniculate nucleus (dLGN) and corresponding neurons of young, adult and old rats. The study was carried out on male albino rats aged 3, 18, 24 and 28 months. In order to estimate the volume of the dLGN, neuronal volume density, numerical density and total number of neurons, we used serial sections stained according to the Klüver-Barrera technique and stereological methods. We found that dorsal lateral geniculate nucleus volume increases between 3 and 28 months, with a larger increase between 24 and 28 months. Neuronal volume density and numerical density of neurons are greater at 3 months and undergo a significant decrease between 24 and 28 months. Finally, the total number of neurons is shown to be smaller in adult and old animals than in younger ones, even though no significant variations are found between 18 and 28 months. Furthermore, this study confirms the need to analyze the total number of neurons and not just neuronal density if we want to correctly evaluate some of the microscopic changes occurring during senescence.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Stereological age-related changes in neurons of the rat dorsal lateral geniculate nucleus

Díaz¹,

Villena²,

González³

et al. 1999

Anat. Rec.

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“…Age-related increase in COX-de fi cient cells has been reported in neurons of the substantia nigra , dorsal lateral geniculate nucleus (Diaz et al 1996 ) , hippocampal pyramidal neurons and synapses (Bertoni-Freddari et al 2004 ) , choroid plexus epithelial cells correlating well to cell death and dysfunction in the aging CNS (Cottrell et al 2001 ) .The decreased activity of complexes I and IV by about one-third is close to the limit of a tolerable impairment of mitochondrial function with respect to basal ATP production of about 36% of the mitochondrial mass in metabolic state 3 of respiration (Boveris et al 1999 ) . Under conditions of increased ATP demand, brain mitochondria increase ATP synthesis by switching more mitochondria from the resting state 4 to the active state 3.…”

Section: Agingmentioning

confidence: 96%

Mitochondrial Oxidative Phosphorylation

Kadenbach¹

2012

Advances in Experimental Medicine and Biology

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Effects of aging on neurons and glial cells from the superficial layers of the superior colliculus in rats

Díaz¹,

Moreno²,

Villena³

et al. 2003

Microscopy Res & Technique

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The aim of this study was to investigate the effect of aging on glial cells and neurons from the superficial layers of the superior colliculus in rats. We used stereological methods to estimate the volume of the superficial layers, neuron size, and the number of neurons and glial cells in Wistar male rats aged 3, 24, 26, and 28 months. A 32.6% volume increase was found in the stratum griseum superficiale between the ages of 3 and 26 months, while in the 28-month-old animals a 19% decrease was observed. The stratum opticum did not show any changes in volume with age. Also, our analysis revealed a process of somatic and nuclear atrophy in the neurons of the superficial layers in animals aged 26 and 28 months. On the other hand, no statistically significant differences were found in the numbers of neurons. The number of glial cells in the stratum griseum superficiale showed an increase between the 3rd and 26th month, while the stratum opticum suffered no change.

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Quantitative histochemical study of cytochrome oxidase in the dLGN of aging rats

Cited by 8 publications

References 27 publications

Stereological age-related changes in neurons of the rat dorsal lateral geniculate nucleus

Stereological age-related changes in neurons of the rat dorsal lateral geniculate nucleus

Mitochondrial Oxidative Phosphorylation

Effects of aging on neurons and glial cells from the superficial layers of the superior colliculus in rats

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