Quantitative imaging of natural products in fine brain regions using desorption electrospray ionization mass spectrometry imaging (DESI-MSI): Uncaria alkaloids as a case study

Gao, Lei; Zhang, Zijia; Wu, Wenyong; Deng, Yanping; Zhi, Haijuan; Long, Huali; Lei, Min; Hou, Jinjun; Wu, Wanying; Guo, De‐An

doi:10.1007/s00216-022-04130-3

Cited by 14 publications

(6 citation statements)

References 52 publications

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“…The ability to perform rapid tissue imaging has become one of the major applications in AIMS research, with DESI‐MS being the most popular technique for this purpose. The past 12 months have seen an abundance of studies harnessing DESI for imaging of biological tissues, particularly in murine models, such as imaging of alkaloids in rat brains, 134 visualizing the distribution of carcinogens in the kidneys of rats, 135 and imaging of mouse and rat brains with convolutional neural network‐based deep learning. 136 In addition, numerous modifications to the original DESI technique have emerged to improve mass spectral imaging capabilities.…”

Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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The development of ambient ionization mass spectrometry (AIMS) has transformed analytical science, providing the means of performing rapid analysis of samples in their native state, both in and out of the laboratory. The capacity to eliminate sample preparation and pre‐MS separation techniques, leading to true real‐time analysis, has led to AIMS naturally gaining a broad interest across the scientific community. Since the introduction of the first AIMS techniques in the mid‐2000s, the field has exploded with dozens of novel ion sources, an array of intriguing applications, and an evident growing interest across diverse areas of study. As the field continues to surge forward each year, ambient ionization techniques are increasingly becoming commonplace in laboratories around the world. This annual review provides an overview of AIMS techniques and applications throughout 2022, with a specific focus on some of the major fields of research, including forensic science, disease diagnostics, pharmaceuticals and food sciences. New techniques and methods are introduced, demonstrating the unwavering drive of the analytical community to further advance this exciting field and push the boundaries of what analytical chemistry can achieve.

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Section: Applications Of Aimsmentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2022: An annual review

Rankin‐Turner

Sears

Heaney

2023

Analytical Science Advances

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“…These alkaloids were isolated from Uncaria species (Rubiaceae), a traditional Chinese medicine (TCM) for treating CNS diseases. The distribution trend of these Uncaria alkaloids and the R/S-configuration epimer influence were summarized to provide valuable SAR information [102]. Sun et al used MALDI-MSI to successfully map distributions of notoginsenoside-R1, notoginsenoside-Fc, ginsenoside-Re in the rhizome, and root of Panax notoginseng (Figure 9b), a highly valuable herbal medicine used in Asian for the treatment of cardiovascular and hematologic diseases.…”

Section: Msi For Medicinal Plant and Natural Productmentioning

confidence: 99%

Mass spectrometry imaging advances and application in pharmaceutical research

Song

Meng

2022

Acta Materia Medica

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Mass spectrometry imaging (MSI) has been shown to be a valuable tool through nearly every stage of the preclinical drug research and development (R&D) pipeline, and even to the early phase of clinical pharmaceutical evaluation. MSI can specifically resolve distributions of a parent drug and its metabolic products across dosed specimens without loss of spatial information, thus facilitating the direct observation of a drug’s pharmacokinetic processes, such as absorption, distribution, metabolism, and excretion. MSI can simultaneously visualize hundreds of phenotype molecules, including proteins, glycans, metabolites, and lipids, which have unique distribution patterns and biofunctions across different physiologic regions. This featured specificity in the chemical and physical spaces empowers MSI as an ideal analytical technique in exploring a drug’s pharmacodynamic properties, including in vitro/in vivo efficacy, safety, potential toxicity, and possible molecular mechanism. The application of MSI in pharmaceutical research has also been expanded from the conventional dosed tissue analysis to the front end of the preclinical drug R&D pipeline, such as investigating the structure-activity relationship, high-throughput in vitro screening, and ex vivo studies on single cells, organoids, or tumor spheroids. This review summarizes MSI application in pharmaceutical research accompanied by its technical and methodologic advances serving this central demand.

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“…The study by Gao et al provides more depth in the quantitative analysis. In an experiment to quantify the distribution of alkaloids by DESI-MS, they obtained results of the distribution of the seven Uncaria alkaloids in 13 brain regions by means of standard curves, and vincamine were used as internal standards to cover the sections uniformly, which effectively compensated for matrix effects, and by imaging analysis they found an enrichment of alkaloids in the pineal gland for the first time (L. Gao et al, 2022).…”

Section: Safety Evaluation Of Natural Medicinesmentioning

confidence: 99%

Advances in mass spectrometry imaging for toxicological analysis and safety evaluation of pharmaceuticals

Chen

Xie

et al. 2022

Mass Spectrometry Reviews

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Safety issues caused by pharmaceuticals have frequently occurred worldwide, posing a tremendous threat to human health. As an essential part of drug development, the toxicological analysis and safety evaluation is of great significance. In addition, the risk of pharmaceuticals accumulation in the environment and the monitoring of the toxicity from natural medicines have also received ongoing concerns. Due to a lack of spatial distribution information provided by common analytical methods, analyses that provide spatial dimensions could serve as complementary safety evaluation methods for better prediction and evaluation of drug toxicity. With advances in technical solutions and software algorithms, mass spectrometry imaging (MSI) has received increasing attention as a popular analytical tool that enables the simultaneous implementation of qualitative, quantitative, and localization without complex sample pretreatment and labeling steps. In recent years, MSI has become more attractive, powerful, and sensitive and has been applied in several scientific fields that can meet the safety assessment requirements. This review aims to cover a detailed summary of the various MSI technologies utilized in the biomedical and pharmaceutical area, including technical principles, advantages, current status, and future trends. Representative applications and developments in the safety‐related issues of different pharmaceuticals and natural medicines are also described to provide a reference for pharmaceutical research, improve rational clinical medicine use, and ensure public safety.

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Quantitative imaging of natural products in fine brain regions using desorption electrospray ionization mass spectrometry imaging (DESI-MSI): Uncaria alkaloids as a case study

Cited by 14 publications

References 52 publications

Applications of ambient ionization mass spectrometry in 2022: An annual review

Applications of ambient ionization mass spectrometry in 2022: An annual review

Mass spectrometry imaging advances and application in pharmaceutical research

Advances in mass spectrometry imaging for toxicological analysis and safety evaluation of pharmaceuticals

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