Quantitative Imaging Using Autoradiographic Techniques

Autoradioluminography, a powerful and reliable tool for drug development: Accelera's experience

“…A drawback of tritium‐labelled compounds is the low radiochemical stability due to the chemical behavior of tritium, that in aqueous solution have a tendency to replace one of the stable hydrogen atoms of water, resulting in tritiated water ([ 3 H]‐H 2 0). This phenomenon is particularly relevant in vivo: 3 H‐label is often less biologically stable than 14 C labeling and it is more difficult to predict its metabolic stability and therefore potential risk of the in vivo formation of 3 H 2 O should be considered during data analysis . As consequence is good practice to monitor compound stability before any in vivo study.…”

“…This phenomenon is particularly relevant in vivo: 3 H-label is often less biologically stable than 14 C labeling and it is more difficult to predict its metabolic stability and therefore potential risk of the in vivo formation of 3 H 2 O should be considered during data analysis. 15 As consequence is good practice to monitor compound stability before any in vivo study. A way to evaluate the phenomena is the comparison between the extent of radioactivity in fresh samples (plasma or/and urine) and the radioactivity recovered after samples evaporation/lyophilization: when the amount of radioactivity in dried samples is significantly lower than the one observed in wet samples, the effect of tritium exchange could be relevant and upcoming experimental results must be corrected and/or interpreted with attention.…”