Quantitative immunoassay of Treponema denticola serovar C in adult periodontitis

Simonson, Lloyd G.; Goodman, C H; Morton, H. E.

doi:10.1128/jcm.28.7.1493-1496.1990

Cited by 38 publications

(29 citation statements)

References 8 publications

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“…77 dentteota, a spirochete that can be cultured in vitro from subgingival plaques, was originally grouped into at least 3 serovars on the basis of the sero-logical agglutination test and fluorescent immunoassay with absorbed antisera (4). The serovars A, B and C are denoted as ATCC 35405, ATCC 33521 and ATCC 33520 (or ATCC 35404), respectively (21,23,34). Our study showed that the ATCC 33520 and 35404 strains carried the common P3, P4, P5, P6, P9, PIO, PI 1 and P14 polypeptide antigens.…”

Section: Acknowledgementsmentioning

confidence: 71%

“…Discontinuous SDS-PAGE was conducted on the basis of the method of Laemmli as reported previously (II). A mixture of each bacterial suspension (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) fig of protein per lane) and solubilization buffer was heated at 100 C for 5 min and applied to 1-or 2-mmthick SDS-10% acrylamide gels. A total of 13 polypeptides were selected on the basis of individual quantity, clear separation from neighboring protein bands and exclusion of the antigens with apparently similar molecular sizes.…”

Section: Sds-page and Polyclonai Antisera Productionmentioning

confidence: 99%

“…We previously reported that a strainspecific 53 kDa polypeptide of T. denticola ATCC 33520 was located on the sur-face of the outer envelopes (28,29). Other antigenic proteins were also found in human oral spirochetes and were utilized as probes or indicators to examine their associations with periodontal diseases (8,16,23,24,34).…”

mentioning

confidence: 99%

“…In the study of oral spirochetes as well as other periodontal pathogens, monoclonal antibodies have been used for immunological analysis, including cross-reactivity among a group of bacterial species or strains (8,23,24,28), The production of a monoclonal antibody, however, usually requires much labor. On the other hand, with immunoblot analysis using antisera against whole bacterial cells, it is sometimes very difficult to detect detailed antigenic specificity to the antibodies because the potential antigens from each bacterial cell produce different kinds of antibodies (5).…”

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confidence: 99%

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Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins

Nakamura

Umemoto

Nakatani

et al. 1993

Oral Microbiology and Immunology

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Thirteen polypeptide antigens with molecular weights ranging from 34 kDa to 83 kDa were selected and their antigenic behaviors and distribution were examined in 12 strains of microorganisms including Treponema, Borrelia, Leptospira and Leptonema. Immunoblot analysis demonstrated that 45 kDa and 83 kDa polypeptides of Treponema socranskii subsp. buccale ATCC 35534, 53 kDa antigen of Treponema denticola ATCC 33520 and 44 kDa polypeptide of the strain G7201 were strain-specific. The 34, 62, 66 and 84 kDa polypeptide antigens were detected in all 8 treponemal strains examined. T. denticola ATCC 33520 and ATCC 35404 possessed 38 kDa, 48 kDa, 52 kDa and 72 kDa common polypeptide antigens. All 12 strains possessed the 84 kDa polypeptide antigen. Immunoelectron microscopy demonstrated that the 34 kDa and 38 kDa polypeptide antigens were located on the axial flagella and that other polypeptide antigens were located on the outer envelopes or wall-membrane complexes.

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Section: Acknowledgementsmentioning

confidence: 71%

Section: Sds-page and Polyclonai Antisera Productionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins

Nakamura

Umemoto

Nakatani

et al. 1993

Oral Microbiology and Immunology

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show abstract

“…We reported that a strain-specific 53,000-(53-kDa) antigen of T denticola American Type Culture Collection (ATCC) strain is located on the surface of the outer envelopes and that this major polypeptide might share with hexagonally arranged polygonal structure each possessing three pores in the outer envelopes (18,19). Some antigenic proteins were also found in human oral spirochetes and were utilized as probes or indicators to examine their associations with periodontal diseases (9,13,14,21). These types of antigenic proteins may be located in their axial flagella as well as on the outer envelopes.…”

Section: Strainsmentioning

confidence: 99%

Antigenic Behaviors of Two Axial Flagellar Proteins Detected in Treponema denticola

Umemoto

Wadood

Nakamura

et al. 1993

Microbiology and Immunology

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Two polypeptide antigens with molecular sizes of 34,000 daltons (34 kDa) and 38 kDa. were separated from heated cells of a human clinical treponeme strain G7201 and Treponema denticola ATCC 35404, respectively. The rabbit polglonal antisera against these antigens were produced and examined for their immunological reactions with the two heated antigens or intact spirochetal cells. Immunoblot analysis showed that the 34-kDa protein was also detected in T. denticola ATCC 35404 and ATCC 33520, and the 38-kDa protein was detected only in the two ATCC strains. Immunoelectron microscopy using the two rabbit antisera and protein A-gold complexes demonstrated that the 38-kDa protein antigen was present on the axial flagella of two strains, and that the 34-kDa protein was located in the axial flagella of the G7201cell, but neither in axial flagella nor on outer envelopes of the two ATCC strains cells, suggesting that the native 34-kDa axial flagellar protein of the G7201 strain may be different from that of T. denticola in terms of immunological reactivity.

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Treponematosis and Lyme borreliosis connections: Explanation for Tchefuncte disease syndromes?

Lewis

1994

American J Phys Anthropol

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A convergence of evidence from macroscopic, radiographic and histologic examination indicates that treponemal infection was present in the 16ST1 Tchefuncte Indian burial population, dated 500 B.C. to 300 A.D. Pattern and nature of lesions suggests that chronic infection induced by variants of the spirochete Treponema pallidum, causing endemic syphilis and/or yaws, resulted in third-stage osseous response. It is suggested that Tchefuncte Indians acquired partial immunity to treponemal infection by exposure to a variant of the related spirochete, Borrelia burgdorferi, the causative agent of Lyme disease. Partial immunity would help explain the relatively mild expression of the treponemal disease process in the 16ST1 skeletal population and the apparent absence of venereal syphilis. Presence of the Borrelia burgdorferi spirochete might be linked to a single incidence of juvenile rheumatoid arthritis.

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Quantitative immunoassay of Treponema denticola serovar C in adult periodontitis

Cited by 38 publications

References 8 publications

Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins

Common and specific antigens of several treponemes detected by polyclonal antisera against major cellular proteins

Antigenic Behaviors of Two Axial Flagellar Proteins Detected in Treponema denticola

Treponematosis and Lyme borreliosis connections: Explanation for Tchefuncte disease syndromes?

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