Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium

Yudkovski, Yana; Rogowska-Wrzesińska, Adelina; Yankelevich, Irena; Shefer, Edna; Herut, Barak; Tom, Moshe

doi:10.1016/j.marenvres.2008.02.001

Cited by 18 publications

(6 citation statements)

References 37 publications

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“…Hepatic MT synthesis is induced by a number of metals, cytokines and stress hormones as well as by a wide range of chemicals, many of which act indirectly via stress or inflammatory response (Coyle et al 2002). MT transcript and protein are induced in fish by a variety of bivalent metals, including Cd, Zn, Cu, Pb and Hg (Ikebuchi et al 1986;Carginale et al 1998;Riggio et al 2003b;Eroglu et al 2005;Alvarado et al 2006;Yudkovski et al 2008). Therefore, MTs have become of great interest for assessing pollution in the marine environment and are seen as potential biomarkers of metal exposure in fish (Georgen and Olssen 1994;Langston et al 2002;Sarkar et al 2006;Nesto et al 2007;Fernandes et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems

Creti

Trinchella

Scudiero

2009

Environ Monit Assess

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The distribution and potential bioaccumulation of dietary and waterborne cadmium and lead in tissues of sea bream (Sparus aurata), a major aquaculture species, was studied in relation to three different fish farming systems. Metallothionein levels in fish tissues were also evaluated. Results demonstrate that metal concentrations in various tissues significantly vary among fish culture systems. Different tissues show different capacity for accumulating heavy metals. The content of both cadmium and lead is not strictly correlated with that of metallothionein. Indeed, the marked accumulation of both metals in liver, as well as the high lead content found in gills and kidney, are not accompanied by a concomitant accumulation of metallothioneins in these tissues. No correlation is present between heavy metals and metallothionein content in muscle tissue. The results also demonstrate that cadmium accumulates mainly via dietary food, whereas lead accumulation is not of food origin. Noteworthy is that the concentration of the two metals found in muscle in all instances is lower than the limits established by European Union legislation for fish destined for human consumption.

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Section: Introductionmentioning

confidence: 99%

Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems

Creti

Trinchella

Scudiero

2009

Environ Monit Assess

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“…Very similar results were presented in other study [28], in which it was shown that most of cadmium (60%) was located in the heat stable cytosolic component, probably bound by metallothioneins, protecting the liver from cadmium toxicity. In this study [29], the authors paid their attention to cadmium distribution in yellow perch ( Perca flavescens ) liver. Perches were under several natural long-term exposure of water-born cadmium.…”

Section: Resultsmentioning

confidence: 99%

Effect of Cadmium Chloride on Metallothionein Levels in Carp

Kovářová

Kizek

Adam

et al. 2009

Sensors

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Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals, we can obviously observe considerable toxicity. It is well known that organisms treated with heavy metals synthesize low molecular mass compounds rich in cysteine. In this work the effects of cadmium chloride (2.5, 5, 7.5, 10 and 12.5 mg/L) on common carp (Cyprinus carpio) was investigated. We determined cadmium content in tissue of muscle, liver and kidney by atomic absorption spectrometry with electrothermal atomization and content of metallothionein (MT) in the same tissues by the Brdicka reaction. Electrochemical methods can be considered as suitable and sensitive tools for MT determination in carp tissues. Results of our study showed a gradually enhancing of cadmium content in muscle with time and dose of cadmium chloride in water. MT levels in liver reached both high levels (above 130 ng/g) in fish exposed to 2.5, 5 and 7.5 mg/L and low level (to 50 ng/g) in fish exposed to 10 and 12.5 mg/L of cadmium chloride. This finding confirms that the synthesis of metallothioneins and binding capacity of these proteins is restricted.

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“…Yudkovski et al . (2008) also reported that MT transcript induction appeared only upon exposure to relatively high levels of Cd, not generally existing in the coastal environment or in rivers. However, acclimatization to elevated levels of trace elements may be achieved by altered uptake or elimination rates (Dallinger et al ., 1996).…”

Section: Complications In Use Of Thiols As Biomarkersmentioning

confidence: 96%

“…Yudkovski et al . (2008) reported that Cd is accumulated in the liver and its further transportation to other organs is limited. It seems that the chronic exposure which may lead to steady state Cd hepatic levels requires long-term exposure to high Cd levels.…”

Section: Cadmium – Uptake and Toxicokineticsmentioning

confidence: 99%

Can thiol compounds be used as biomarkers of aquatic ecosystem contamination by cadmium?

Kovářová¹,

Svobodová²

2009

Interdisciplinary Toxicology

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Due to anthropogenic activities, heavy metals still represent a threat for various trophic levels. If aquatic animals are exposed to heavy metals we can obviously observe considerable toxicity. It is well known that an organism affected by cadmium (Cd) synthesize low molecular mass thiol compounds rich in cysteine (Cys), such as metallothioneins (MT) and glutathione (GSH/GSSG). The aim of this study was to summarize the effect of Cd on level of thiol compounds in aquatic organisms, and evaluate that the concentrations of thiol compounds are effective indicators of Cd water pollution and explain their potential use in biomonitoring applications.

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Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium

Abstract: International audienc

Cited by 18 publications

References 37 publications

Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems

Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems

Effect of Cadmium Chloride on Metallothionein Levels in Carp

Can thiol compounds be used as biomarkers of aquatic ecosystem contamination by cadmium?

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