Quantitative increases of extracellular vesicles in prolonged cold storage of platelets increases the potential to enhance fibrin clot formation

Nash, J; Davies, Angela M.; Saunders, C.; George, C E; Williams, J O; James, Philip E.

doi:10.1111/tme.12989

Cited by 5 publications

(1 citation statement)

References 55 publications

(114 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In part, this capability appears to be due to lower temperature slowing down platelets’ metabolism, resulting in priming or greater aggregation once in vivo [ 67 ]. This greater hemostatic capacity also results from production of a higher number of large platelet-derived extracellular vesicles that decrease the time needed for clot formation [ 69 ]. Furthermore, this greater state of activation is a direct response to observed increases in cytosolic calcium released from the endoplasmic reticulum [ 70 ].…”

Section: Alternatives To Room Temperature Storagementioning

confidence: 99%

Bacterial Contamination of Platelet Products

Jacobs,

Zhou,

Tayal

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Transfusion of bacterially contaminated platelets, although rare, is still a major cause of mortality and morbidity despite the introduction of many methods to limit this over the past 20 years. The methods used include improved donor skin disinfection, diversion of the first part of donations, use of apheresis platelet units rather than whole-blood derived pools, primary and secondary testing by culture or rapid test, and use of pathogen reduction. Primary culture has been in use the US since 2004, using culture 24 h after collection of volumes of 4–8 mL from apheresis collections and whole-blood derived pools inoculated into aerobic culture bottles, with limited use of secondary testing by culture or rapid test to extend shelf-life from 5 to 7 days. Primary culture was introduced in the UK in 2011 using a “large-volume, delayed sampling” (LVDS) protocol requiring culture 36–48 h after collection of volumes of 16 mL from split apheresis units and whole-blood derived pools, inoculated into aerobic and anaerobic culture bottles (8 mL each), with a shelf-life of 7 days. Pathogen reduction using amotosalen has been in use in Europe since 2002, and was approved for use in the US in 2014. In the US, recent FDA guidance, effective October 2021, recommended several strategies to limit bacterial contamination of platelet products, including pathogen reduction, variants of the UK LVDS method and several two-step strategies, with shelf-life ranging from 3 to 7 days. The issues associated with bacterial contamination and these strategies are discussed in this review.

show abstract

Section: Alternatives To Room Temperature Storagementioning

confidence: 99%

Bacterial Contamination of Platelet Products

Jacobs,

Zhou,

Tayal

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

Impact of production methods and storage conditions on extracellular vesicles in packed red blood cells and platelet concentrates

Ebeyer-Masotta,

Eichhorn,

Fischer

et al. 2024

Transfusion and Apheresis Science

View full text Add to dashboard Cite

Lipidomic changes occurring in platelets during extended cold storage

Green,

Padula,

Dodgen

et al. 2024

Transfusion Medicine

View full text Add to dashboard Cite

ObjectivesCold storage is being implemented as an alternative to conventional room‐temperature storage for extending the shelf‐life of platelet components beyond 5–7 days. The aim of this study was to characterise the lipid profile of platelets stored under standard room‐temperature or cold (refrigerated) conditions.MethodsMatched apheresis derived platelet components in 60% PAS‐E/40% plasma (n = 8) were stored at room‐temperature (20–24°C with agitation) or in the cold (2–6°C without agitation). Platelets were sampled on day 1, 5 and 14. The lipidome was assessed by ultra‐pressure liquid chromatography ion mobility quadrupole time of flight mass spectrometry (UPLC IMS QToF). Changes in bioactive lipid mediators were measured by ELISA.ResultsThe total phospholipid and sphingolipid content of the platelets and supernatant were 44 544 ± 2915 μg/mL and 38 990 ± 10 880 μg/mL, respectively, and was similar over 14 days, regardless of storage temperature. The proportion of the procoagulant lipids, phosphatidylserine (PS) and phosphatidylethanolamine (PE), increased by 2.7% and 12.2%, respectively, during extended cold storage. Cold storage for 14 days increased sphingomyelin (SM) by 4.1% and decreased ceramide by 1.6% compared to day 1. Further, lysophosphatidylcholine (LPC) species remained unchanged during cold storage for 14 days. The concentration of 12‐ and 15‐hydroxyeicosatetraenoic acid (HETE) were lower in the supernatant of cold‐stored platelets than room‐temperature controls stored for 14 days.ConclusionThe lipid profile of platelets was relatively unchanged during storage for 5 days, regardless of temperature. However, during extended cold storage (14 days) the proportion of the procoagulant lipids, PS and PE, increased, while LPC and bioactive lipids were stable.

show abstract

Quantitative increases of extracellular vesicles in prolonged cold storage of platelets increases the potential to enhance fibrin clot formation

Cited by 5 publications

References 55 publications

Bacterial Contamination of Platelet Products

Bacterial Contamination of Platelet Products

Impact of production methods and storage conditions on extracellular vesicles in packed red blood cells and platelet concentrates

Lipidomic changes occurring in platelets during extended cold storage

Contact Info

Product

Resources

About