Quantitative investigation of cardiomyocyte hypertrophy and myocardial fibrosis over 6 years after cardiac transplantation

Armstrong, Arthur T.; Binkley, Philip F.; Baker, Peter B.; Myerowitz, P. David; Leier, Carl V.

doi:10.1016/s0735-1097(98)00296-4

Cited by 97 publications

(79 citation statements)

References 14 publications

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“…Interstitial ET-1 was associated with a lower cellular rejection score, suggesting a dissociation between rejection and fibrosis, as has been shown in pediatric liver transplants. 14 The concept of fibrosis after transplantation has been thoroughly examined by Armstrong and his colleagues, 15 who evaluated serial endomyocardial biopsies on 50 cardiac transplant patients followed over 5 years. The investigators showed that myocardial fibrosis develops early and remains modestly elevated 2 months after transplant, indicating that peri-transplant factors might be responsible for this fibrotic process.…”

Section: Discussionmentioning

confidence: 99%

Patterns of Myocardial Endothelin-1 Expression and Outcome After Cardiac Transplantation

et al. 2002

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Background-Endothelin-1 (ET-1), a potent vasoconstrictor, is released in response to several inflammatory cytokines after heart transplantation. The present study correlated patterns of myocardial ET-1 expression in heart biopsies with acute rejection, post-transplantation ischemic injury, and subsequent development of coronary vasculopathy. Methods and Results-Patterns of myocardial ET-1 expression were evaluated in 47 heart transplant recipients at 3 months after transplant. Transplant vasculopathy was documented by coronary angiography at 2 years after transplant. Expression of ET-1 was tabulated for both blood vessels and the interstitium. Vascular ET-1 expression was positive in 7/17 (41%) of patients with greater than grade 2 (International Society Heart Lung Transplant) rejection compared with 3/30 (10%) of patients with grade 0 and grade 1A rejection (Pϭ0.02). Compared with patients with negative interstitial ET-1 expression (nϭ22), patients with positive interstitial ET-1 expression (nϭ25) had higher incidence of post-transplantation ischemic injury (52% versus 9%, Pϭ0.002), lower mean episodes of acute rejection (Ն grade 2) during the first 3 months of transplant (1.09Ϯ0.66 versus 1.86Ϯ1.6, Pϭ0.048), and more common vasculopathy at 2 years (50% versus 15%, Pϭ0.02), and they tended to have worse survival (83.2% versus 100%, Pϭ0.058). Conclusions-Vascular ET-1 expression is likely to be associated with acute rejection. Interstitial ET-1 expression, however, is more likely to be associated with post-transplantation ischemic injury and subsequent development of coronary vasculopathy.

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Section: Discussionmentioning

confidence: 99%

Patterns of Myocardial Endothelin-1 Expression and Outcome After Cardiac Transplantation

et al. 2002

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“…After counterstaining with Harris hematoxylin (Sigma  ) for 25 seconds, the slides were mounted and analyzed by light microscopy. Photomicrographs were analyzed by LGMC-image software version 1.0 and the fractional percentages of the immunoreactive area to aorta and myocardium CD40L were acquired (Armstrong et al, 1998).…”

Section: Histological and Immunohistochemical Proceduresmentioning

confidence: 99%

Propolis and swimming in the prevention of atherogenesis and left ventricular hypertrophy in hypercholesterolemic mice

et al. 2015

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Aims: The present study verified the effect of propolis alone and its association with swimming in dyslipidemia, left ventricular hypertrophy and atherogenesis of hypercholesterolemic mice. Methods and Results: The experiments were performed in LDLr-/-mice, fed with high fat diet for 75 days, and were divided into four experimental groups (n=10): HL, sedentary, subjected to aquatic stress (5 min per day, 5 times per week); NAT submitted to a swimming protocol (1 hour per day, 5 times per week) from the 16 th day of the experiment; PRO, sedentary, submitted to aquatic stress and which received oral propolis extract (70 uL/animal/day) from the 16 th day of the experiment; HL+NAT+PRO, submitted to swimming and which received propolis as described above. After 75 days, blood was collected for analysis of serum lipids. The ratio between the ventricular weight (mg) and the animal weight (g) was calculated. Histological sections of the heart and aorta were processed immunohistochemically with anti-CD40L antibodies to evaluate the inflammatory process; stained with hematoxylin/eosin and picrosirius red to assess morphological and morphometric alterations. The HL animals showed severe dyslipidemia, atherogenesis and left ventricular hypertrophy, associated with a decrease in serum HDLc levels and subsequent development of cardiovascular inflammatory process, characterized by increased expression of CD40L in the left ventricle and aorta. Swimming and propolis alone and\or associated prevented the LVH, atherogenesis and arterial and ventricular inflammation, decreasing the CD40L expression and increasing the HDLc plasmatic levels. Conclusion: Propolis alone or associated with a regular physical activity is beneficial in cardiovascular protection through anti-inflammatory action.Keywords: dyslipidemia, left ventricular hypertrophy, propolis, LDLr-/-mice. Própolis e natação na prevenção da aterogênese e hipertrofia ventricular esquerda de camundongos hipercolesterolêmicos ResumoObjetivos: O presente estudo verificou o efeito do própolis associação ou não com a natação na dislipidemia, na hipertrofia ventricular esquerda e aterogênese de camundongos hipercolesterolêmicos. Métodos e Resultados: Os experimentos foram realizados em camundongos LDLr-/-, alimentados com dieta hiperlipídica por 75 dias, e divididos em quatro grupos experimentais (n = 10): HL, sedentários, foram submetidos ao estresse aquático (5 min por dia, cinco vezes por semana); NAT foram submetidos a um protocolo de natação (1 hora por dia, cinco vezes por semana) a partir do 16° dia do experimento; PRO, sedentários, submetidos a estresse aquático e que receberam extrato de própolis oral (70 uL / animal / dia) a partir do 16° dia do experimento; HL + NAC + PRO, submetidos a natação e que recebeu a própolis, como descrito acima. Após 75 dias, foi coletado sangue para análise do perfil lipídico. Calculou-se a relação entre o peso ventricular (mg) e o peso do animal (g). Os cortes histológicos do coração e aorta foram processados imunohistoquímicamente co...

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“…All of these aspects may also play an important role in post-heart transplant patients being frequently influenced by risk factors of diastolic dysfunction such as hypertension, diabetes mellitus, obesity, and metabolic syndrome [11][12][13][14][15][16] . As demonstrated previously 11 and confirmed in this study, systemic hypertension is a very common finding in patients after heart transplantation.…”

Section: Aspects Of Left Ventricular Filling Pressure Elevation and Dmentioning

confidence: 99%

High prevalence of exercise-induced heart failure with normal ejection fraction in post-heart transplant patients

Meluzı́n¹,

Hude²,

Leinveber³

et al. 2014

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aim. Post-heart transplant patients are at increased risk of diastolic dysfunction. The aim of this study was to assess the prevalence of isolated only exercise-induced heart failure with normal ejection fraction (HFNEF) in heart transplant recipients. Methods and Results. To determine pulmonary capillary wedge pressure (PCWP) at rest and during exercise, 81 patients after orthotopic heart transplantation with normal left ventricular ejection fraction (LVEF) underwent exercise right heart catheterization with simultaneous exercise echocardiography. Based on PCWP values, the patients were divided into three groups. Twenty-one patients had no evidence of HFNEF (PCWP at rest < 15 mmHg, maximal PCWP during exercise < 25 mmHg, prevalence 26%). Forty-seven subjects were found to have only exercise-induced HFNEF (PCWP at rest < 15 mmHg, maximal PCWP during exercise ≥ 25 mmHg, prevalence 58%). Thirteen patients had HFNEF already at rest (PCWP ≥ 15 mmHg at rest, prevalence 16%). Of the noninvasive parameters obtained at rest, multivariate regression analysis identified LV mass index adjusted for allograft age to be an independent predictor of exercise-induced HFNEF. Conclusions. In heart transplant recipients with normal LVEF, there is a high prevalence of exercise-induced HFNEF. LV mass index adjusted for allograft age is predictive of exercise-induced HFNEF.

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Quantitative investigation of cardiomyocyte hypertrophy and myocardial fibrosis over 6 years after cardiac transplantation

Cited by 97 publications

References 14 publications

Patterns of Myocardial Endothelin-1 Expression and Outcome After Cardiac Transplantation

Patterns of Myocardial Endothelin-1 Expression and Outcome After Cardiac Transplantation

Propolis and swimming in the prevention of atherogenesis and left ventricular hypertrophy in hypercholesterolemic mice

High prevalence of exercise-induced heart failure with normal ejection fraction in post-heart transplant patients

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