Quantitative investigation of the mechanisms of microplastics and nanoplastics toward zebrafish larvae locomotor activity

Chen, Qiqing; Gundlach, Michael J.; Yang, Shouye; Jiang, Jia; Velki, Mirna; Yin, Daqiang; Hollert, Henner

doi:10.1016/j.scitotenv.2017.01.156

Cited by 582 publications

(224 citation statements)

References 66 publications

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“…Yet, our results did not show any alteration of the LPMR by any of the different MPs preparations. Similar results were shown after exposure of zebrafish embryos to pure polystyrene MPs particles by Chen et al (2017), who spiked polystyrene MPs with EE2 (17α-ethinylestradiol) and found hypoactivity at elevated concentrations only. However, previous studies showed that microplastics may reduce other behavioral parameters such as predatory performance of fish depending on the shape of plastic particles (de Sá et al, 2015;Choi et al, 2018).…”

Section: Larval Behaviorsupporting

confidence: 77%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

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Section: Larval Behaviorsupporting

confidence: 77%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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“…The abnormality in REM sleep was shown by the mutant mice, followed with other abnormalities, including reward-related and locomotor responses to psychostimulants and analgesic responses [114]. Besides, the hypoactivity behavior of fish, in this case, might also be attributed to an increase in oxidative damage [2]. However, this hypoactivity behavior that was observed during this test was in contrast with the hyperactivity behavior shown during the novel tank test.…”

Section: Behavioral and Neurotransmitters Alterations Caused By Ps-npsmentioning

confidence: 69%

“…Macroplastic is clearly visible plastic that can be caught and generally will not have a direct impact on the food chain. Farm animals or fish that mistake them for food swallow the majority of these tiny plastic particles and, thus, can find their way into the human body [2].…”

Section: Introductionmentioning

confidence: 99%

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Sarasamma

Audira

Siregar

et al. 2020

IJMS

273

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Plastic pollution is a growing global emergency and it could serve as a geological indicator of the Anthropocene era. Microplastics are potentially more hazardous than macroplastics, as the former can permeate biological membranes. The toxicity of microplastic exposure on humans and aquatic organisms has been documented, but the toxicity and behavioral changes of nanoplastics (NPs) in mammals are scarce. In spite of their small size, nanoplastics have an enormous surface area, which bears the potential to bind even bigger amounts of toxic compounds in comparison to microplastics. Here, we used polystyrene nanoplastics (PS-NPs) (diameter size at ~70 nm) to investigate the neurobehavioral alterations, tissue distribution, accumulation, and specific health risk of nanoplastics in adult zebrafish. The results demonstrated that PS-NPs accumulated in gonads, intestine, liver, and brain with a tissue distribution pattern that was greatly dependent on the size and shape of the NPs particle. Importantly, an analysis of multiple behavior endpoints and different biochemical biomarkers evidenced that PS-NPs exposure induced disturbance of lipid and energy metabolism as well as oxidative stress and tissue accumulation. Pronounced behavior alterations in their locomotion activity, aggressiveness, shoal formation, and predator avoidance behavior were exhibited by the high concentration of the PS-NPs group, along with the dysregulated circadian rhythm locomotion activity after its chronic exposure. Moreover, several important neurotransmitter biomarkers for neurotoxicity investigation were significantly altered after one week of PS-NPs exposure and these significant changes may indicate the potential toxicity from PS-NPs exposure. In addition, after ~1-month incubation, the fluorescence spectroscopy results revealed the accumulation and distribution of PS-NPs across zebrafish tissues, especially in gonads, which would possibly further affect fish reproductive function. Overall, our results provided new evidence for the adverse consequences of PS-NPs-induced behavioral dysregulation and changes at the molecular level that eventually reduce the survival fitness of zebrafish in the ecosystem.

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“…intestinal damage and change in metabolic profiles) (Chen et al . , ; Mattsson et al . ; Sussarellu et al .…”

Section: Perniciousness Of Solidsmentioning

confidence: 99%

Generation, characterization, perniciousness, removal and reutilization of solids in aquaculture water: a review from the whole process perspective

Bao

Zhu

Jin

et al. 2018

Reviews in Aquaculture

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The generation of solids by aquaculture activities has led to many problems in animal welfare, environmental quality, water treatment effects and public health, such as gill and intestinal damage, water eutrophication, system components blockages and antibiotics transfers through the food chain to human; thus, effective solid wastes management is very important. The purpose of this review is to summarize the sources, characterization methods, harmfulness, removal technologies and reutilization of solids in aquaculture water, including (i) different sources of solids, such as uneaten feed, feces and debris; (ii) solids characterization based on the general parameters, particle size distribution and chemical composition; (iii) the toxicity and detriment of solids to cultured species, the environment, aquaculture system components and human health; (iv) Solids removal, effluents solids thickening and source control techniques for better solids management; and (v) various methods for the reutilization of aquacultural solid wastes for resource value-adding. In addition to these five specific objectives, the merits and drawbacks of each method or technology and future research priorities are discussed. The present review provides an understanding of the role played by solids in aquaculture water and provides guidance for aquacultural solid wastes precision management, aquaculture practices and technological developments. Thus, solids management can be carried out based on this guidance to improve animal welfare and the stability of water treatment systems, reduce harm to the environment and public health, and enable the development of aquaculture in a sustainable way.

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Quantitative investigation of the mechanisms of microplastics and nanoplastics toward zebrafish larvae locomotor activity

Cited by 582 publications

References 66 publications

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Generation, characterization, perniciousness, removal and reutilization of solids in aquaculture water: a review from the whole process perspective

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