Quantitative Label-Free Comparison of the Metabolic Protein Fraction in Old and Modern Italian Wheat Genotypes by a Shotgun Approach

Francesco, Antonella Di; Cunsolo, Vincenzo; Saletti, Rosaria; Svensson, Birte; Muccilli, Vera; Vita, Pasquale De; Foti, Salvatore

doi:10.3390/molecules26092596

Cited by 9 publications

(12 citation statements)

References 58 publications

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“…At this stage, the protein content was calculated performing a fluorimetric assay with the Qubit Protein Assay Kit (Qubit 1.0 Fluorometer;Thermo Fisher Scientific). Finally, an amount of sample equivalent to 10 μg of quantified proteins was reduced by adding 0.1 M, 1,4-dithiothreitol in 50 mM NH₄HCO₃ pH 8.2 (3 h, RT), alkylated with 0.2 M iodoacetamide in the same buffer solution (in the dark at RT for 1 h) and digested overnight at 37°C by a solution of porcine trypsin (Sequencing Grade Modified Trypsin, Porcine, Promega, USA) corresponding to an enzyme–substrate ratio of 1:50 [ 42 ]. The peptide mixture solutions were vacuum dried (Concentrator Plus; Eppendorf, Germany), dissolved in 100 μl of H 2 O + 5% formic acid (FA), and analyzed by Reversed Phase-nano-High-Performance Liquid Chromatography-nano-Electrospray Ionization-tandem mass spectrometry (RP-nHPLC/nESI-MS/MS).…”

Section: Methodsmentioning

confidence: 99%

“…Mass spectrometry data were acquired on a Thermo Fisher Scientific Orbitrap Fusion Tribrid® (Q-OT-qIT) mass spectrometer (Thermo Fisher Scientific) as elsewhere reported [ 42 ]. Liquid chromatography was carried out with a Thermo Scientific Dionex UltiMate 3000 RSLC nanosystem (Sunnyvale, CA) first loading the peptide mixtures onto a trapping column (Acclaim® Nano-Trap C18 Column; 100 μm i.d.…”

Section: Methodsmentioning

confidence: 99%

“…The elution was achieved with a flow rate of 0.25 μl/min at 40°C. The eluting peptide cations were converted to gas-phase ions by electrospray ionization using the same instrumental parameters reported in [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

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Nano-structured myelin: new nanovesicles for targeted delivery to white matter and microglia, from brain-to-brain

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Palumbo

Federico

et al. 2021

Materials Today Bio

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Neurodegenerative diseases affect millions of people worldwide and the presence of various physiological barriers limits the accessibility to the brain and reduces the efficacy of various therapies. Moreover, new carriers having targeting properties to specific brain regions and cells are needed in order to improve therapies for the brain disorder treatment. In this study, for the first time, Myelin nanoVesicles (hereafter defined MyVes) from brain-extracted myelin were produced. The MyVes have an average diameter of 100–150 nm, negative zeta potential, spheroidal morphology, and contain lipids and the key proteins of the myelin sheath. Furthermore, they exhibit good cytocompatibility. The MyVes were able to target the white matter and interact mainly with the microglia cells. The preliminary results here presented allow us to suppose the employment of MyVes as potential carrier to target the white matter and microglia in order to counteract white matter microglia-related diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Nano-structured myelin: new nanovesicles for targeted delivery to white matter and microglia, from brain-to-brain

Picone

Palumbo

Federico

et al. 2021

Materials Today Bio

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“…6-8 kDa [23,29,30]. It has been found that the level of SCIs is regulated in wheat according to the cultivar, showing significant differences between wheat genotypes [31].…”

Section: Plant Protease Inhibitors: Classification and Biological Functionsmentioning

confidence: 99%

Intestinal Exposure to Food-Derived Protease Inhibitors: Digestion Physiology- and Gut Health-Related Effects

et al. 2021

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Plant-derived protease inhibitors (PI), such as Bowman-Birk inhibitors and Kunitz-type inhibitors, have been suggested to negatively affect dietary protein digestion by blocking the activity of trypsin and chymotrypsin in the human gastrointestinal system. In addition, some PIs may possess proinflammatory activities. However, there is also scientific evidence on some beneficial effects of PIs, for example, gut-related anti-inflammatory and chemopreventive activities in vitro and in vivo. Some PIs are sensitive to processing and digestion; thus, their survival is an important aspect when considering their positive and negative bioactivities. The aim of this review was to evaluate the relevance of PIs in protein digestion in humans and to discuss the potential of PIs from whole foods and as purified compounds in decreasing symptoms of bowel-related conditions. Based on the reviewed literature, we concluded that while the complex interactions affecting plant protein digestibility and bioavailability remain unclear, PI supplements could be considered for targeted purposes to mitigate inflammation and gastric pain.

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“…Other molecular markers, such as RFLPs and SSRs, were successfully used for the characterization of durum wheat germplasm collections, enabling variations and resources of landraces to be more accessible for exploitation [ 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. More analyses were carried out by markers based on morphological descriptors, storage protein composition, digestibility of starch, and concentration of secondary metabolites, permitting discrimination between new elite varieties and landraces [ 8 , 35 , 36 , 37 , 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization among and within Sicilian Tetraploid Wheat Landraces by Gluten Protein Analysis for Traceability Purposes

Palombieri,

Bonarrigo,

Potestio

et al. 2024

Plants

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The criteria of “Distinctness, Uniformity and Stability” as well as a high “overall quality index” are used to register the Italian modern varieties to the national register. Differently, local conservation varieties can be certified under different EU Directives that facilitate, as an overall objective, the preservation of biodiversity and the containment of genetic erosion. In recent years, products derived from ancient grains are perceived to be healthier and more sustainable by consumers, especially in Italy, with consequent higher market prices. The ancient tetraploid wheat varieties registered in the national register of conservation varieties amount to 28, 24 of which are Sicilian. They are supposed to have wide genetic variability compared to modern ones, making them vulnerable to fraud because they are difficult to trace. It is therefore important to have tools able to discriminate between autochthonous Sicilian varieties. This can be completed by gluten proteins composition, which also provides information on the technological properties of derived products. Fifty-one accessions belonging to twenty-two ancient varieties of Sicilian tetraploid (mostly durum) wheat were analyzed. Although wide intra-accession and intra-varietal variability measurements were assessed, the gliadin pattern of bulks of seeds belonging to each variety was discriminatory. Moreover, differences in technological attitudes were found between landraces. This paves the way to use gluten protein patterns for traceability, allowing local farmers and producers to valorize their products and assure consumers regarding the transparency of the entire supply chain.

show abstract

Quantitative Label-Free Comparison of the Metabolic Protein Fraction in Old and Modern Italian Wheat Genotypes by a Shotgun Approach

Cited by 9 publications

References 58 publications

Nano-structured myelin: new nanovesicles for targeted delivery to white matter and microglia, from brain-to-brain

Nano-structured myelin: new nanovesicles for targeted delivery to white matter and microglia, from brain-to-brain

Intestinal Exposure to Food-Derived Protease Inhibitors: Digestion Physiology- and Gut Health-Related Effects

Characterization among and within Sicilian Tetraploid Wheat Landraces by Gluten Protein Analysis for Traceability Purposes

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