Quantitative LC–HRMS determination of selected cardiovascular drugs, in dried blood spots, as an indicator of adherence to medication

Bernieh, Dennis; Lawson, Graham; Tanna, Sangeeta

doi:10.1016/j.jpba.2017.04.045

Cited by 27 publications

(34 citation statements)

References 28 publications

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“…5, an extraction temperature of 40°C is sufficient for the effective extraction of atenolol from DPS. This is consistent with data from work [18] where the authors sonicated samples of DBS for 30 mins at 40°C.…”

Section: Resultssupporting

confidence: 91%

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Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Chernonosov

Koval

2019

JPRI

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Aims: Atenolol is one of the β-blockers widely used for the treatment of hypertension and other cardiovascular diseases. To simplify the methods for determining of drugs concentrations in blood and plasma the dried spots assays (dried blood spots or dried plasma spots) could be used. In this case high sensitive detector like mass-spectrometer is required as well as high level of drug recovery from dried spot. In this study the extraction of atenolol from dried plasma spots (DPS) was studied to offer the optimum parameters of extraction method. Study Design: Short research articles. Place and Duration of Study: Core Facility of Mass Spectrometric Analysis, Institute of Chemical Biology and Fundamental Medicine SB RAS, between January and October 2019. Methodology: The organic extraction method was chosen for evaluation as the most suitable for LC-MS assay. Several parameters: % of organic solvent, presence or absence of 0.1% formic acid, Short Research Articletime, volume and temperature of extraction were investigated to find the best combination for atenolol recovery from DPS for further LC Results: Results showed that the solvent composition and temperature has main influence on the extraction. The effect of extraction time and volume of solvent have no significant influence on atenolol recovery. Pure acetonitrile is the worst solvent for atenolol extraction from DPS. The solvents: MeOH:H 2 O (60:40, v:v), MeOH:0.1% FA in H v:v) or ACN:MeOH (60:40, v:v) provide the best recovery of atenolol temperature is 40°C, time of extraction is 15 Conclusion: Several solvents acceptable for LC from DPS can be used for routine extraction of atenolol.

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Section: Resultssupporting

confidence: 91%

“…Acetonitrile and methanol are most suitable solvents for using in further LC-MS analysis. Therefore for atenolol extraction from DBS researchers used 150 L, MeOH:H 2 O (70:30, v/v) [17], 300 L, MeOH [18], 200 L, MeOH:H 2 O (60:40, v/v) [4], 500 L of ACN:MeOH (1:2, v/v).…”

Section: Resultsmentioning

confidence: 99%

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Chernonosov

Koval

2019

JPRI

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“…In this case, 1000 μ L of MeOH served as an extraction solvent with 98–99 % recovery. The assay sensitivity for valsartan was better than that reported in studies on MS detection in TOF mode [ 49 , 50 ]. Irbesartan has been detected in MRM mode only as an internal standard (IS), but losartan detection in the MRM mode enables investigators to achieve LOQ of 1 ng/mL [ 55 ].…”

Section: Arasmentioning

confidence: 66%

“…These drugs block the peripheral conversion of angiotensin I to angiotensin II [ 77 ]. The DBS technique has predominantly been used by Tanna and coauthors for quantification of ramipril [ 48 , 50 , 51 ], captopril [ 53 ], and lisinopril [ 50 ]. Ramipril is the most studied medication among ACE inhibitors and is stable up to 84 days at RT with good recovery (~90%) from Whatman 903 and Ahlstrom 226 cards.…”

Section: Ace Inhibitorsmentioning

confidence: 99%

“…The only difference in the elution procedure was the extraction solvent: 300 μ L of methanol (MeOH) [ 50 ] or 150 μ L of MeOH/H 2 O (70:30, v/v) [ 48 , 49 , 51 ], and the latter showed better recovery. Lisinopril was analyzed in the same assay [ 50 ] in TOF mode at 406.2336 m/z. Captopril has been quantitated with similar results in SRM and TOF modes [ 53 ].…”

Section: Ace Inhibitorsmentioning

confidence: 99%

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The Use of Dried Blood Spots for the Quantification of Antihypertensive Drugs

Chernonosov

2018

International Journal of Analytical Chemistry

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Hypertension or high blood pressure is a harbinger of cardiovascular diseases. There are several classes of drugs used to treat hypertension. This review discusses the use of dried blood spots (DBSs) for the quantification by mass spectrometry (MS), tandem mass spectrometry (MS/MS), or, in some cases, by fluorescence detection methods the following antihypertensive medications: angiotensin-converting enzyme inhibitors (ramipril, ramiprilat, captopril, and lisinopril); angiotensin II receptor antagonists (valsartan, irbesartan, losartan, and losartan carboxylic acid); calcium channel blockers (verapamil, amlodipine, nifedipine, pregabalin, and diltiazem); α blockers (guanfacine, doxazosin, and prazosin); β blockers (propranolol, bisoprolol, atenolol, and metoprolol); endothelin receptor antagonists (bosentan and ambrisentan); and statins (simvastatin, atorvastatin, and rosuvastatin).

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A rapid and sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of atorvastatin calcium and lisinopril in rat plasma and its application in a pharmacokinetic study

Jia,

Bao,

Hou

et al. 2023

Separation Science Plus

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Atorvastatin calcium and lisinopril are commonly used to reduce cholesterol and control blood pressure in the clinic. In this study, a sensitive and rapid method was developed and validated for the simultaneous detection of plasma concentrations of atorvastatin calcium and lisinopril using an ultra‐high performance liquid chromatography‐tandem mass spectrometry with an ACQUITY UPLC BEH C18 column. The mobile phase of methanol and 0.1% formic acid aqueous solution was pumped at a flow rate of 0.2 mL/min. The retention times of atorvastatin calcium and lisinopril were 2.48 min and 1.99 min, respectively. The linear range of atorvastatin calcium in plasma samples was 1–2000 ng/mL, while that of lisinopril was 5–2000 ng/mL. The absolute values of intraday and inter‐day precision and accuracy were all below 15%. Furthermore, the recovery and matrix effects of atorvastatin calcium, lisinopril, and nimodipine were 80%–120% and 85%‐115%, respectively. Therefore, the developed method can be applied for the simultaneous quantification of atorvastatin calcium and lisinopril in rat plasma. The Cmax were about 199 ng/mL and 2059 ng/mL for atorvastatin calcium and lisinopril, respectively. In addition, the T1/2 of atorvastatin calcium and lisinopril were 0.6 ± 0.3 and 5.4 ± 1.7 h, respectively.

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Quantitative LC–HRMS determination of selected cardiovascular drugs, in dried blood spots, as an indicator of adherence to medication

Cited by 27 publications

References 28 publications

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

Extraction Procedure Optimization of Atenolol from Dried Plasma Spots

The Use of Dried Blood Spots for the Quantification of Antihypertensive Drugs

A rapid and sensitive ultra‐high‐performance liquid chromatography‐tandem mass spectrometry method for the simultaneous determination of atorvastatin calcium and lisinopril in rat plasma and its application in a pharmacokinetic study

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