Quantitative LC–MS determination of liposomal encapsulated prednisolone phosphate and non-encapsulated prednisolone concentrations in murine whole blood and liver tissue

Smits, Evelien A. W.; Soetekouw, José A.; Doormalen, Irene van; Berg, Bart H. J. van den; Woude, Marcel P. van der; Wijs-Rot, Nicolette de; Vromans, Herman

doi:10.1016/j.jpba.2015.07.012

Cited by 9 publications

(4 citation statements)

References 36 publications

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“…Smits et al [50] used LC-MS/MS technology to determine liposomal-encapsulated prednisolone phosphate and non-encapsulated prednisolone in whole blood and liver tissue. Prednisolone phosphate is rapidly dephosphorylated by phosphatases in vivo and converted into prednisolone.…”

Section: Quantitative Methods For Liposomesmentioning

confidence: 99%

Analytical methods for investigating in vivo fate of nanoliposomes: A review

Liu

Yang³

et al. 2018

Journal of Pharmaceutical Analysis

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Nanoliposomes are considered to be the most successful nanoparticle drug delivery system, but their fate in vivo has not been fully understood due to lack of reliable bioanalytical methods, which seriously limits the development of liposomal drugs. Hence, an overview of currently used bioanalytical methods is imperative to lay the groundwork for the need of developing a bioanalytical method for liposome measurements in vivo. Currently, major analytical methods for nanoliposomes measurement in vivo include fluorescence labeling, radiolabeling, magnetic resonance imaging (MRI), mass spectrometry and computed tomography. In this review, these bioanalytical methods are summarized, and the advantages and disadvantages of each are discussed. We provide insights into the applicability and limitations of these analytical methods in the application of nanoliposomes measurement in vivo, and highlight the recent development of instrumental analysis techniques. The review is devoted to providing a comprehensive overview of the investigation of nanoliposomes design and associated fate in vivo, promoting the development of bioanalytical techniques for nanoliposomes measurement, and understanding the pharmacokinetic behavior, effectiveness and potential toxicity of nanoliposomes in vivo.

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Section: Quantitative Methods For Liposomesmentioning

confidence: 99%

Analytical methods for investigating in vivo fate of nanoliposomes: A review

Liu

Yang³

et al. 2018

Journal of Pharmaceutical Analysis

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“…The collected blood and tissue samples were processed and analyzed according to previously developed and validated methodology for the quantitative differentiation of encapsulated PP and released P in murine whole blood and liver tissue [27, 35]. The suitability of this methodology for tumor, kidney and splenic tissue was verified by qualification (internal study similar to the validation as described by Smits et al [35]). As discussed in the introduction, the encapsulation of a phosphate prodrug like PP into liposomes enables the direct and accurate quantification of encapsulated and released drug in tissues: as long as the compound is encapsulated it is assessed as a phosphate.…”

Section: Methodsmentioning

confidence: 99%

“…After the samples were treated with high intensity focused ultrasound to disrupt the cell membranes, PP and P concentrations were measured by LC-MS analysis. The used chromatographic conditions were as described by Smits et al [35]. Comprehensive liquid chromatography together with negative electrospray ionization, insource-fragmentation of P and D and high-resolution accurate mass Orbitrap-MS analysis was used to avoid the significant interference by (biological) impurities from the complex matrix during PP and P detection.…”

Section: Methodsmentioning

confidence: 99%

The availability of drug by liposomal drug delivery

et al. 2018

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SummaryLately, the usefulness of liposomal drug delivery systems has been debated. To better understand the underlying pharmacokinetics of the targeted drug delivery by liposomes, individual encapsulated and non-encapsulated drug concentrations in blood, tumor, liver, spleen and kidneys were quantified after i.v. administration of liposomal prednisolone phosphate in mice. Kinetic analysis shows that the tumor influx of encapsulated drug is not dominant compared to the uptake by the other tissues. Further, from a quantitative point of view, the availability of non-encapsulated drug in the tumor tissue after liposomal delivery is not pronounced as compared to the other tissues studied. However, drug release in the tumor seems more extended than in the other tissues and the non-encapsulated drug concentration decreases more slowly in the tumor than in the liver and spleen. The spleen shows a high affinity for the uptake of encapsulated drug as well as the release of drug from the liposomes. Subsequently, released drug in the spleen, and possibly also in other tissues, is probably quickly redistributed towards the blood and other tissues. This also impairs the drug delivery effect of the liposomes. In contrast to the released drug in the central circulation, liver and spleen, the released drug concentration in the tumor remains at a fairly constant level likely due to the extended release kinetics from the liposomes. These extended release characteristics in the tumor most probably contribute to the beneficial effect. Nevertheless, it should be noted that larger released drug concentrations are formed in healthy tissues.Electronic supplementary materialThe online version of this article (10.1007/s10637-018-0708-4) contains supplementary material, which is available to authorized users.

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“…To separate biotin-DTX-liposomes from plasma, streptavidin-Fe 3 O4@PDA was used since its particular binding with biotin and streptavidin is so efficient. Through the presence of a magnetic field in this process, an incredible 75% recovery efficiency has been achieved [30][31][32][33][34][35][36].…”

Section: Lc-ms/msmentioning

confidence: 99%

Comprehensive review on nano drug delivery systems bioanalysis

Sujeevan¹

2023

GSC Adv. Res. Rev.

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Nanoparticulate drug delivery systems (NDDSs) offer a revolutionary strategy to fighting ailments. The NDDSs are uniquely and deliberately constructed as vessels for the transport of active pharmaceutical components (APIs), which leverages their distinct physicochemical traits, such as lengthened circulation period, enhanced targeting capability and evasion of drug resistance. Although the last three decades have seen considerable progress, our comprehension of how NDDSs' pharmacokinetics in vivo can affect their safety profiles is still insufficient. Examining NDDSs is way more complex than analyzing small molecules since their structure and composition are completely different, almost making regular analytical techniques inadequate for measuring correctly its behavior within living systems. The following advanced bioanalysis techniques are summarized to trace the in vivo fate of NDDSs: liquid chromatography tandem mass spectrometry (LC-MS/MS), enzyme-linked immunosorbent assay (ELISA), magnetic resonance imaging (MRI), radiolabeling, fluorescence spectroscopy, laser ablation inductively coupled plasma MS (LA-ICP–MS,) and size exclusion chromatography. These tools provide an invaluable way of examining a drug's journey through the body. By leveraging the most modern technologies, we are able to conduct a thorough survey of NDDSs and their various changes in structure, composition and form. This review can be beneficial for accurately assessing pharmacokinetics as well as gauging efficacy and safety profiles of new drug delivery systems. We hope this comprehensive overview will serve as an effective basis for application methodology when analyzing NDDSs.

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Quantitative LC–MS determination of liposomal encapsulated prednisolone phosphate and non-encapsulated prednisolone concentrations in murine whole blood and liver tissue

Cited by 9 publications

References 36 publications

Analytical methods for investigating in vivo fate of nanoliposomes: A review

Analytical methods for investigating in vivo fate of nanoliposomes: A review

The availability of drug by liposomal drug delivery

Comprehensive review on nano drug delivery systems bioanalysis

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