Quantitative lipid biomarker detection of unculturable microbes and chlorine exposure in water distribution system biofilms

Smith, Carol A.; Phiefer, Charles B.; Macnaughton, Sarah J.; Peacock, Aaron; Burkhalter, Robert S.; Kirkegaard, R.D.; White, David C.

doi:10.1016/s0043-1354(00)00028-2

Cited by 46 publications

(37 citation statements)

References 17 publications

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“…This confirms that the maximum number of sequences generated in this projects contains uncultured microorganism [19]. For the cultivable mocroorganisms, lots of work has been done on biomarkers development, even to identify the water quality aslo [20].…”

Section: Taxonomical Analysissupporting

confidence: 73%

Determination of Uncultured Microorganisms Based on Metagenomics Signature or Biomarkers at Fresh Water Reservoirs

Panchal¹,

Bhairavkar²,

Patil³

2018

OAJS

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Metagenomics is the branch of science which does the direct analysis of genetic material of environmental samples. Maximum microorganisms present in the ecosystem are unculturable. Metagenomics with the expansion in the field of Next Generation Sequencing (NGS) has generated large amount of raw sequence reads of uncultured microbes from different ecosystem. This combination opens door for new metabolites, drug targets, new species and unknown world of new genes could be utilized for the therapeutics. Biomarkers could be utilized as an indicator for the identification of any biological processes, disease genes, metabolites, enzymes, and antibiotics while biosensor is an analytical devise for the detection of analyte with the help of biological component (biomarkers) and physicochemical detector. Current study tried to find probable biomarkers which could be utilized for biosensor development especially for uncultured microorganisms from fresh water ecosystems. We collected all fresh water raw reads from publically available different repositories. After preliminary screening on huge data sets, we performed quality analysis on raw read sequences, generated 10 different conserve domains by multiple sequence alignment. These CDs could be promising biomarkers for the identification of uncultured microbes from fresh water ecosystems which may be pathogenic for humans. DNA hybridization based Biosensor could be synthesized by using by using this biomarker sequences for detecting the presence of uncultured microorganisms (pathogenic/non-pathogenic) in fresh water ecosystem.

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Section: Taxonomical Analysissupporting

confidence: 73%

Determination of Uncultured Microorganisms Based on Metagenomics Signature or Biomarkers at Fresh Water Reservoirs

Panchal¹,

Bhairavkar²,

Patil³

2018

OAJS

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“…The combined phospholipid fatty acid (PLFA) and lipopolysaccharide 3-hydroxy fatty acid (LPS 3-OH-FA) analyses have earlier been used to describe microbial community structures and biomass in soils (51,52) and sediments (7). PLFAs have been studied from the biological filters used for drinking water treatment (25,26,27) and from water distribution system biofilms exposed to chlorine (36). Our aim was to use the PLFA and LPS 3-OH-FA analyses to determine whether the addition of phosphate would change the microbial community structure in drinking water biofilms.…”

mentioning

confidence: 99%

The Microbial Community Structure of Drinking Water Biofilms Can Be Affected by Phosphorus Availability

Keinänen

Korhonen²,

Lehtola³

et al. 2002

Appl Environ Microbiol

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Microbial communities in biofilms grown for 4 and 11 weeks under the flow of drinking water supplemented with 0, 1, 2, and 5 g of phosphorus liter ؊1 and in drinking and warm waters were compared by using phospholipid fatty acids (PLFAs) and lipopolysaccharide 3-hydroxy fatty acids (LPS 3-OH-FAs). Phosphate increased the proportion of PLFAs 16:17c and 18:17c and affected LPS 3-OH-FAs after 11 weeks of growth, indicating an increase in gram-negative bacteria and changes in their community structure. Differences in community structures between biofilms and drinking and warm waters can be assumed from PLFAs and LPS 3-OH-FAs, concomitantly with adaptive changes in fatty acid chain length, cyclization, and unsaturation.

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“…The emitter biofilm biomass and microbial community were analyzed for phospholipids fatty acids (PLFAs). This method provides an unbiased view of the complex microbial communities and viable biomass found in environmental samples such as soils (Ratcliff et al, 2006), sediments (Ringelberg et al, 1997), and drinking water system biofilms (Smith et al, 2000;Keinänen et al, 2004). This method is based on the assumption that phospholipids make up a relatively constant proportion of the cell biomass and that fatty acid variation among taxonomic groups provides markers that can be used to interpret community-level profiles (Zhang et al, 2008).…”

Section: Analysis Methodsmentioning

confidence: 99%

Biofilm Accumulation and Structure in the Flow Path of Drip Emitters Using Reclaimed Wastewater

Ding¹,

Yang²,

Rowan³

et al. 2010

Transactions of the ASABE

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Quantitative lipid biomarker detection of unculturable microbes and chlorine exposure in water distribution system biofilms

Cited by 46 publications

References 17 publications

Determination of Uncultured Microorganisms Based on Metagenomics Signature or Biomarkers at Fresh Water Reservoirs

Determination of Uncultured Microorganisms Based on Metagenomics Signature or Biomarkers at Fresh Water Reservoirs

The Microbial Community Structure of Drinking Water Biofilms Can Be Affected by Phosphorus Availability

Biofilm Accumulation and Structure in the Flow Path of Drip Emitters Using Reclaimed Wastewater

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