Quantitative magnetic resonance (QMR) method for bone and whole-body-composition analysis

Taicher, Gersh Z.; Tinsley, Frank C.; Reiderman, Arcady; Heiman, Mark L.

doi:10.1007/s00216-003-2224-3

Cited by 236 publications

(202 citation statements)

References 52 publications

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“…Previous studies in rodents have used physical methods, such as whole-body and regional dissection and analysis of fat deposits (42-44), or histological (microscopic) analysis of tissues (45). Whereas chemical analysis and dissection methods have been used for determining fat content in rodents, recent studies indicate that quantitative MRI provides more reliable measurements of fat content in rats and mice (43,46). Particularly in smaller animals, where dissection cannot easily separate organs and regional boundaries, noninvasive MRI techniques for measuring fat volume and distribution are preferable (46).…”

Section: Study Design and Methodologymentioning

confidence: 99%

“…Whereas chemical analysis and dissection methods have been used for determining fat content in rodents, recent studies indicate that quantitative MRI provides more reliable measurements of fat content in rats and mice (43,46). Particularly in smaller animals, where dissection cannot easily separate organs and regional boundaries, noninvasive MRI techniques for measuring fat volume and distribution are preferable (46). They provide detailed anatomical information in addition to fat distribution maps (45).…”

Section: Study Design and Methodologymentioning

confidence: 99%

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Altered Upper Airway and Soft Tissue Structures in the New Zealand Obese Mouse

Brennick¹,

Pack²,

Ko³

et al. 2009

Am J Respir Crit Care Med

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Rationale: The effect of obesity on upper airway soft tissue structure and size was examined in the New Zealand Obese (NZO) mouse and in a control lean mouse, the New Zealand White (NZW). Objectives: We hypothesized that the NZO mouse has increased volume of neck fat and upper airway soft tissues and decreased pharyngeal airway caliber. Methods: Pharyngeal airway size, volume of the upper airway soft tissue structures, and distribution of fat in the neck and body were measured using magnetic resonance imaging (MRI). Dynamic MRI was used to examine the differences in upper airway caliber between inspiration and expiration in NZO versus NZW mice. Measurements and Main Results:The data support the hypothesis that, in obese NZO versus lean NZW mice, airway caliber was significantly smaller (P , 0.03), with greater parapharyngeal fat pad volumes (P , 0.0001) and a greater volume of other upper airway soft tissue structures (tongue, P 5 0.003; lateral pharyngeal walls, P 5 0.01; soft palate, P 5 0.02). Dynamic MRI showed that the airway of the obese NZO mouse dilated during inspiration, whereas in the lean NZW mouse, the upper airway was reduced in size during inspiration. Conclusions: In addition to the increased volume of pharyngeal soft tissue structures, direct fat deposits within the tongue may contribute to airway compromise in obesity. Pharyngeal airway dilation during inspiration in NZO mice compared with narrowing in NZW mice suggests that airway compromise in obese mice may lead to muscle activation to defend upper airway patency during inspiration.

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Section: Study Design and Methodologymentioning

confidence: 99%

Section: Study Design and Methodologymentioning

confidence: 99%

Altered Upper Airway and Soft Tissue Structures in the New Zealand Obese Mouse

Brennick¹,

Pack²,

Ko³

et al. 2009

Am J Respir Crit Care Med

View full text Add to dashboard Cite

show abstract

“…Total body fat mass and lean mass were measured by magnetic resonance imaging using Bruker Minispec, as described (26). The subcutaneous, mesenteric, gonadal, and retroperitoneal adipose tissues were carefully dissected from each mouse and weighed before being flash-frozen in liquid nitrogen.…”

Section: Body Composition and Adipose Depotsmentioning

confidence: 99%

Genetic and hormonal control of hepatic steatosis in female and male mice

Norheim

Hui

Kulahcioglu

et al. 2017

Journal of Lipid Research

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“…15,16 This instrument creates a contrast between adipose tissue, muscle and free body fluids by taking advantage of the differences in relaxation times of the hydrogen spins and hydrogen density in these tissues. Each magnetic resonance measurement per animal was repeated two to three times and the median was used for further analyses.…”

Section: Phenotypesmentioning

confidence: 99%

A unique genetic defect on chromosome 3 is responsible for juvenile obesity in the Berlin Fat Mouse

et al. 2010

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Objective: This study aimed at the mapping and estimation of genetic and sex effects contributing to the obese phenotype of the Berlin Fat Mouse Inbred line 860 (BFMI860). This mouse line is predisposed for juvenile obesity. BFMI860 mice accumulate 24% total fat mass at 10 weeks of age under a standard maintenance diet. Design: A total of 471 mice of a (BFMI860 Â C57BL/6NCrl) F 2 intercross population were fed a standard maintenance diet and were analysed for body composition at 10 weeks when they finished their rapid growth phase. Results: The most striking result was the identification of a novel obesity locus on chromosome 3 (Chr 3) at 40 Mb, explaining 39% of the variance of total fat mass in the F 2 population under a standard diet. This locus was named jObes1 (juvenile obesity 1). The BFMI860 allele effect was recessive. Males and females homozygous at jObes1 had on average 3.0 and 3.3 g more total fat mass at 10 weeks than the other two genotype classes, respectively. The effect was evident in all white adipose tissues, brown adipose tissue and also in liver. The position of the Chr 3 effect is syntenic to an obesity locus in humans. Additional loci for total fat mass and different white adipose tissue weights with minor effects were detected on mouse Chr 5 and 6. Another locus on Chr 4 had influence especially on liver weight. Many loci including jObes1 affected males and females to a different extent. Conclusion:The major locus on Chr 3 for juvenile obesity and its interaction with sex is unique and makes the BFMI860 mice an interesting resource for the discovery of novel genetic factors predisposing obesity, which might also contribute to obesity in humans. The results suggested that metabolic and regulatory pathways differed between the sexes.

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Quantitative magnetic resonance (QMR) method for bone and whole-body-composition analysis

Cited by 236 publications

References 52 publications

Altered Upper Airway and Soft Tissue Structures in the New Zealand Obese Mouse

Altered Upper Airway and Soft Tissue Structures in the New Zealand Obese Mouse

Genetic and hormonal control of hepatic steatosis in female and male mice

A unique genetic defect on chromosome 3 is responsible for juvenile obesity in the Berlin Fat Mouse

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