Quantitative mapping of elemental distribution in leaves of the metallophytes Helichrysum candolleanum, Blepharis aspera, and Blepharis diversispina from Selkirk Cu–Ni mine, Botswana

Koosaletse-Mswela, Pulane; Przybyłowicz, W.J.; Cloete, Karen J.; Barnabas, Alban D.; Torto, Nelson; Mesjasz‐Przybyłowicz, Jolanta

doi:10.1016/j.nimb.2015.09.005

Cited by 11 publications

(4 citation statements)

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“…A unique place in science has been assigned to plants called metallophytes, i.e. plants accumulating heavy metals in their tissues [26]. They become a source of food for phytophagous insects, especially of the species that specialize in this group of plants, although it was detected that the part of plant species use heavy metals as protection against herbivores [27].…”

Section: Discussionmentioning

confidence: 99%

Bioaccumulation of Heavy Metals (Zn, Pb, Cd) in <i>Polistes nimphus</i> (Christ, 1791) (Hymenoptera, Vespidae) Living on Contaminated Sites

Mielczarek¹,

Wojciechowicz-Żytko²

2020

Pol. J. Environ. Stud.

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The aim of the study was to determine the extent of heavy metal accumulation (Zn, Cd and Pb) in the bodies of females of the predatory species Polistes nimphus (Christ, 1791) (Hymenoptera, Vespidae). The insects were captured in areas affected by the proximity of ZGH "Bolesław" -an industrial complex located in Bukowno near Olkusz (southern Poland), whose main activity is mining and processing of lead and zinc ores.Three sites that differed in terms of distance from the source of contamination and also in the concentrations of Zn, Cd and Pb in the top soil layer were selected. The heavy metal content of the soil was determined for each site. The most contaminated site was located in the immediate vicinity of ZGH "Bolesław" (4326.50 mg/kg Zn, 56.96 mg/kg Cd, 3977.00 mg/kg Pb); the least contaminated was the site furthest away from the source of contamination (48.75 mg/kg Zn, 0.72 mg/kg Cd, 25.43 mg/kg Pb).On all the sites, during the two-year study (2015)(2016), individuals of the genus Polistes were captured and female wasps of the species P. nimphus were isolated from among them. Then the extent of accumulation of Zn, Cd and Pb in their bodies was determined. Correlations between the concentrations of Cd, Zn and Pb in the soil and those in insect bodies were calculated.On all three sites, in both years of the study, the concentrations of heavy metals in insect bodies changed depending on their concentrations in the soil. The highest levels of the accumulated Zn, Cd and Pb were always observed on the site located in the vicinity of ZGH "Bolesław". The lowest values were observed on the site furthest away from the source of contamination. The concentrations of all three metals in the bodies of insects increased with their increasing concentrations in the soil, but the differences were not statistically significant.

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Section: Discussionmentioning

confidence: 99%

Bioaccumulation of Heavy Metals (Zn, Pb, Cd) in <i>Polistes nimphus</i> (Christ, 1791) (Hymenoptera, Vespidae) Living on Contaminated Sites

Mielczarek¹,

Wojciechowicz-Żytko²

2020

Pol. J. Environ. Stud.

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“…Two options have been successfully applied so far: (1) collecting whole live plants with soil in the field and rapid transport to a cryo‐preparation laboratory (e.g. Koosaletse‐Mswela et al ., ); or (2) cryo‐fixing specimens in the field using metal‐mirror impact freezing and transport to a laboratory in a cryoshipper (Mesjasz‐Przybyłowicz et al ., , Van der Ent et al ., ). Cryoshippers maintain liquid nitrogen temperature (dry vapour) for several days without refilling and are International Air Transport Association‐approved for air transport.…”

Section: Preparation Of Plant Samples For X‐ray Micro‐analysismentioning

confidence: 99%

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

et al. 2017

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Contents Summary432I.Introduction433II.Preparation of plant samples for X‐ray micro‐analysis433III.X‐ray elemental mapping techniques438IV.X‐ray data analysis442V.Case studies443VI.Conclusions446Acknowledgements449Author contributions449References449 Summary Hyperaccumulators are attractive models for studying metal(loid) homeostasis, and probing the spatial distribution and coordination chemistry of metal(loid)s in their tissues is important for advancing our understanding of their ecophysiology. X‐ray elemental mapping techniques are unique in providing in situ information, and with appropriate sample preparation offer results true to biological conditions of the living plant. The common platform of these techniques is a reliance on characteristic X‐rays of elements present in a sample, excited either by electrons (scanning/transmission electron microscopy), protons (proton‐induced X‐ray emission) or X‐rays (X‐ray fluorescence microscopy). Elucidating the cellular and tissue‐level distribution of metal(loid)s is inherently challenging and accurate X‐ray analysis places strict demands on sample collection, preparation and analytical conditions, to avoid elemental redistribution, chemical modification or ultrastructural alterations. We compare the merits and limitations of the individual techniques, and focus on the optimal field of applications for inferring ecophysiological processes in hyperaccumulator plants. X‐ray elemental mapping techniques can play a key role in answering questions at every level of metal(loid) homeostasis in plants, from the rhizosphere interface, to uptake pathways in the roots and shoots. Further improvements in technological capabilities offer exciting perspectives for the study of hyperaccumulator plants into the future.

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“…The recommended starting point for investigations is collecting samples from plants growing in their natural habitat. Collecting plant tissue samples in the field is, however, challenging, but two strategies have been successfully used: (i) collecting whole plants with soil in pots from the metalliferous outcrops and trans-port to a suitably equipped laboratory (Koosaletse-Mswela et al 2015), or (ii) freezing tissue samples in the field using metal-mirror freezing and transport to laboratory in a cryoshipper at liquid nitrogen temperature (Mesjasz-Przybylowicz et al 2016;van der Ent et al 2017). It is important that cryo-fixation is done as soon as possible (i.e.…”

Section: Knowledge Gaps: Priority Regions For Exploration and Discoverymentioning

confidence: 99%

Tools for the Discovery of Hyperaccumulator Plant Species and Understanding Their Ecophysiology

Gei

Erskine

Harris

et al. 2017

Mineral Resource Reviews

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Globally the discovery of hyperaccumulator plants has been hindered by systematic screening of plant species, and is highly biased towards Ni hyperaccumulators. This is mainly due to the existence of a reagent paper test that is only specific to nickel (based on dimethylglyoxime) such that more than 400 of the approximately 500 known hyperaccumulators species are for Ni. New technical advances now permit massive screening of herbarium specimens using non-destructive, portable X-Ray Fluorescence Spectroscopy (XRF), an approach that has already led to the discovery of numerous hyperaccumulator species new to science. The elemental distribution in selected hyperaccumulator plant tissues can then be further studied using techniques such as desktop or synchrotron micro-XRF, nuclear microprobe (PIXE), scanning/transmission electron microscopy with energy-dispersive spectroscopy (SEM/TEM-EDS), secondary ion mass spectrometry (SIMS) or laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS). The use of histochemical dyes combined with light microscopy further aids in the identification of anatomical and structural features of the studied plant tissues.

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Quantitative mapping of elemental distribution in leaves of the metallophytes Helichrysum candolleanum, Blepharis aspera, and Blepharis diversispina from Selkirk Cu–Ni mine, Botswana

Cited by 11 publications

References 23 publications

Bioaccumulation of Heavy Metals (Zn, Pb, Cd) in <i>Polistes nimphus</i> (Christ, 1791) (Hymenoptera, Vespidae) Living on Contaminated Sites

Bioaccumulation of Heavy Metals (Zn, Pb, Cd) in <i>Polistes nimphus</i> (Christ, 1791) (Hymenoptera, Vespidae) Living on Contaminated Sites

X‐ray elemental mapping techniques for elucidating the ecophysiology of hyperaccumulator plants

Tools for the Discovery of Hyperaccumulator Plant Species and Understanding Their Ecophysiology

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