Quantitative Measurement of Alcohol Dehydrogenase Activity within the Liver Lobule of Rats after Prolonged Ethanol Ingestion

Morrison, George R.; Brock, Frances E.

doi:10.1093/jn/92.2.286

Cited by 33 publications

(16 citation statements)

References 17 publications

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“…This suggests that fixation in 4% paraformaldehyde has little effect on the prevention of ADH diffusion. By microdissection, ADH activity was found to be more prominent in the centrilobular zone in the female rat [11,13], but higher in the midlobular zone in the male rat [11]. However, it has been reported that the predominant localization of rat ADH immunoreactive proteins and activity in the centrilobular zone was not affected by gender [20].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is of vital importance to identify the lobular distribution of ADH. However, studies concerning the lobular distribution of ADH have obtained varying results showing predominant centrilobular distribution in rats [8,13,20], mice [17] and humans [3], predominant midlobular distribution in rats [11], predominant periportal distribution in rats [7,21], and an even distribution in rats [2,4,16] and in guinea-pigs [11]. These contradictory findings of the distribution pattern of ADH might result from the diffusion of the soluble enzyme, differing methods or species differences.…”

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confidence: 99%

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Histochemical Localization of Alcohol Dehydrogenase Activities in the Livers of Mice, Rats, Hamsters, and Guinea-Pigs.

Watabiki

Tokiyasu

Yoshida

et al. 1997

Acta Histochem. Cytochem

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Histochemical Localization of Alcohol Dehydrogenase Activities in the Livers of Mice, Rats, Hamsters, and Guinea-Pigs.

Watabiki

Tokiyasu

Yoshida

et al. 1997

Acta Histochem. Cytochem

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“…Studies of the lobular distribution of ADH are based mainly on histochemical (2,8), immunohistochemical (1, 10), or microbiochemical methods (3,4,7), and the results are conflicting. In histochemistry, Greenberger et al (2) have reported a predominance of ADH activity in the portal zone of normal rat livers.…”

Section: Discussionmentioning

confidence: 99%

“…1, 6) may be attributed to whether or not PMS as the intermediate acceptor is present in the incubation medium. In microbiochemical studies, it has been shown that maximum ADH activity in lobules microdissected from normal rat liver was found in the perivenous area in the female (3,4), but in the intermediary zone in the male (3). Vaananen et al (7) found that ADH activity in normal male rat liver was evenly distributed in periportal and perivenous hepatocytes separated by bidirectional collagenase perfusion.…”

Section: Discussionmentioning

confidence: 99%

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Double staining procedure for histochemical localization of alcohol and aldehyde dehydrogenase activities in the mouse liver.

Watabiki

Tokiyasu

Ishida

et al. 1989

Acta Histochem. Cytochem

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The double staining procedure using both the nitroblue tetrazolium (NBT) and copper ferrocyanide methods was presented for the simultaneous localization of two different dehydrogenase activities in a single histologic section.Sections of normal mouse liver were stained for alcohol dehydrogenase (ADH) activity using the NBT method first, then stained for aldehyde dehydrogenase (ALDH) activity using the copper ferrocyanide method. However, the formazan showing ADH activity must chelate metal ion (Cu2+, Co2+, or Ni2+) prior to staining for ALDH activity.Both the blue metal-formazan complex showing ADH and brown copper ferrocyanide showing ALDH activities in a single liver section were localized mainly in the perivenous zone of the hepatic lobule. In addition, ALDH activity is always localized in the zone of the lobule where ADH activity is present.Ethanol is oxidized by alcohol dehydrogenase (ADH, EC 1.1.1.1), and by the metabolite, highly toxic acetaldehyde, aldehyde dehydrogenase (ALDH, EC 1.2.1.3). It seems to be important in which zone of the liver lobule this series of enzymatic activities are localized histochemically. Recently, the ADH activity in mouse liver has been detected principally in the perivenous zone by the nitroblue tetrazolium (NBT) method (8), and the ALDH activity by the copper ferrocyanide method (9). These reaction products, NBT formazan (blue) and copper ferrocyanide (brown), show contrasting colors. If double histochemical staining by these methods is possible in a single section, it will be feasible to know precisely the cytoarchitectural relationship between ADH and ALDH activities.The present study was carried out to establish the methodological basis of dual staining by a combination of the NBT method and the copper ferrocyanide method. In addition, we have also applied the dual staining procedure to the study of the localization of ADH and ALDH activities in a single histologic preparation of mouse liver.401

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UV‐curable methacrylic epoxy dispersions for cationic electrodeposition coating

Kim

Hong

2006

J of Applied Polymer Sci

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UV-curable epoxy dispersions were prepared for cationic electrodeposition coating. Sequential reactions were used to introduce methacrylate groups to the epoxyamine polymer as coupling agents to the multifunctional acrylates. The molecular weight values of the prepared epoxy-amine polymer were M n ¼ 2800 and M w ¼ 4300. The neutralized epoxy-amine polymer containing photoinitiator with or without multifunctional acrylate (pentaerythritol triacrylate, PETA) could be dispersed into a stable dispersion without any phase separation. The size of the particles in these epoxy dispersions was approximately 77.7 nm, and increased with the incorporation of PETA. The electrodeposition process was introduced to the prepared epoxy dispersions, and the electrodeposited films were cured by UV irradiation after a 10-min flash off at 808C. Studies of the kinetics using photo-DSC revealed that the crosslinked films containing PETA gave a higher conversion rate than those without PETA, resulting in better resistance to methyl ethyl ketone.

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Quantitative Measurement of Alcohol Dehydrogenase Activity within the Liver Lobule of Rats after Prolonged Ethanol Ingestion

Cited by 33 publications

References 17 publications

Histochemical Localization of Alcohol Dehydrogenase Activities in the Livers of Mice, Rats, Hamsters, and Guinea-Pigs.

Histochemical Localization of Alcohol Dehydrogenase Activities in the Livers of Mice, Rats, Hamsters, and Guinea-Pigs.

Double staining procedure for histochemical localization of alcohol and aldehyde dehydrogenase activities in the mouse liver.

UV‐curable methacrylic epoxy dispersions for cationic electrodeposition coating

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