Quantitative Measurement of Organic Acids in Tissues from Gastric Cancer Patients Indicates Increased Glucose Metabolism in Gastric Cancer

Hur, Hoon; Paik, Man‐Jeong; Yao, Xuan; Nguyen, Duc-Toan; Ham, In-Hye; Yun, Jisoo; Cho, Yong Kwan; Lee, Gwang; Han, Sang‐Uk

doi:10.1371/journal.pone.0098581

Cited by 49 publications

(29 citation statements)

References 27 publications

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“…Liquid chromatography-mass spectrometry (LC-MS) is the most common method of analysis. Several biomarkers have been proposed for GC diagnosis, prognosis, and surveillance[ 14 - 16 ]. Systematic reviews have demonstrated variation in the relative abundance of the metabolites of glycolysis, lactic acid fermentation, de novo lipid, and amino acid synthesis in biological samples of patients with GC compared with controls[ 13 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolomic alterations and chromosomal instability status in gastric cancer

Tsai

Yeh

et al. 2018

WJG

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AIMTo explore the correlation of metabolomics profiles of gastric cancer (GC) with its chromosomal instability (CIN) status.METHODSNineteen GC patients were classified as CIN and non-CIN type by The Cancer Genome Atlas Research Group system, based on 409 oncogenes and tumor suppressor genes sequenced. The aqueous metabolites of the GC tumor and its surrounding adjacent healthy tissues were identified through liquid chromatography-mass spectrometry. Groups were compared by defining variable importance in projection score of > 1.2, a fold change value or its reciprocal of > 1.2, and a P value of < 0.05 as a significant difference.RESULTSIn total, twelve men and seven women were enrolled, with a median age of 66 years (range, 47-87 years). The numbers of gene alterations in the CIN GC group were significantly higher than those in the non-CIN GC (32-218 vs 2-17; P < 0.0005). Compared with the adjacent healthy tissues, GC tumors demonstrated significantly higher aspartic acid, citicoline, glutamic acid, oxidized glutathione, succinyladenosine, and uridine diphosphate-N-acetylglucosamine levels, but significantly lower butyrylcarnitine, glutathione hydroxyhexanoycarnitine, inosinic acid, isovalerylcarnitine, and threonine levels (all P < 0.05). CIN tumors contained significantly higher phosphocholine and uridine 5’-monophosphate levels but significantly lower beta-citryl-L-glutamic acid levels than did non-CIN tumors (all P < 0.05). CIN GC tumors demonstrated additional altered pathways involving alanine, aspartate, and glutamate metabolism, glyoxylate and dicarboxylate metabolism, histidine metabolism, and phenylalanine, tyrosine, and tryptophan biosynthesis.CONCLUSIONMetabolomic profiles of GC tumors and the adjacent healthy tissue are distinct, and the CIN status is associated with downstream metabolic alterations in GC.

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Section: Introductionmentioning

confidence: 99%

Metabolomic alterations and chromosomal instability status in gastric cancer

Tsai

Yeh

et al. 2018

WJG

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“…In ginseng group, the composition was very similar to that of the normal control. A recent report revealed that the content of several compounds, including malic acid and 3-hydroxybutanoic acid, was significantly higher in tumor tissue compared to normal tissue (40). In our study, very high concentrations of malic acid and 2-hydroxybutanoic acid were also observed in the stool samples of the model group, which may have enhanced carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

American Ginseng Attenuates Colitis-Associated Colon Carcinogenesis in Mice: Impact on Gut Microbiota and Metabolomics

Wang

Xiao

et al. 2016

Cancer Prevention Research

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Inflammatory bowel disease is a risk factor for colorectal cancer (CRC) initiation and development. In this study the effects of American ginseng on chemically-induced colitis and colon carcinogenesis were evaluated using an azoxymethane (AOM)/dextran sulfate sodium (DSS) mouse model. During the acute phase on day 15, the oral administration of ginseng (15 and 30 mg/kg/day) significantly suppressed AOM/DSS-induced colitis, as demonstrated by the disease activity index and colon tissue histology. During the chronic phase in week 13, AOM/DSS-induced tumor multiplicity was significantly suppressed by ginseng. Ginseng significantly attenuated the increase of inflammatory cytokines such as IL-1α, IL-1β, IL-6, G-CSF and GM-CSF. Serum metabolomics data in the PCA plots showed good separation between the AOM/DSS model and ginseng-treated mice, and the most important endogenous metabolite changes were identified. The 16S rRNA data showed that after AOM/DSS, the microbiome community in the model group was obviously changed, and ginseng inhibited these changes. Fecal metabolomics analysis supported these findings. In conclusion, oral ginseng significantly decreased AOM/DSS-induced colitis and colon carcinogenesis by inhibiting inflammatory cytokines, and restoring the metabolomic and microbiota profiles accordingly. Selective endogenous small molecules could be used as biomarkers to elucidate the effects of ginseng treatment.

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“…Normalized levels were then plotted as lines radiating from a common central point. The far ends of the 17 lines for ginseng samples were then joined together to produce star patterns for environmentally friendly and conventionally cultured roots using MS Excel program (Microsoft, Redmond, WA, USA), as described previously …”

Section: Methodsmentioning

confidence: 99%

Monitoring of Organic Acids in Ginseng Roots Produced by Conventional and Environmentally Friendly Farming by Gas Chromatography–Mass Spectrometry

Seo

Kim

et al. 2018

Bulletin Korean Chem Soc

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Metabolomic analysis of organic acids has not been performed for evaluation whether ginseng has been cultivated using conventional or environmental‐friendly farming methods. In this study, profiling analysis was conducted for organic acids (OAs) in ginseng roots produced using conventional or environment‐friendly farming methods at five locations in each of five regions. In OA profiles, lactic acid was the most abundant OA in all regions, with the exception for environmentally friendly farmed ginseng in two of the five regions, in which glycolic acid was most abundant OA. OA profiles in all regions showed isocitric acid levels were increased by environment‐friendly cultivation, which suggests metabolic differences associated from farming method, and that isocitric acid might be a useful discriminatory biomarker of environmental‐friendly and conventional cultivation. The results of the present study suggest metabolomic studies of OAs in ginseng roots might be useful for monitoring whether ginseng has been cultivated using conventional or environmentally friendly farming methods.

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Quantitative Measurement of Organic Acids in Tissues from Gastric Cancer Patients Indicates Increased Glucose Metabolism in Gastric Cancer

Cited by 49 publications

References 27 publications

Metabolomic alterations and chromosomal instability status in gastric cancer

Metabolomic alterations and chromosomal instability status in gastric cancer

American Ginseng Attenuates Colitis-Associated Colon Carcinogenesis in Mice: Impact on Gut Microbiota and Metabolomics

Monitoring of Organic Acids in Ginseng Roots Produced by Conventional and Environmentally Friendly Farming by Gas Chromatography–Mass Spectrometry

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