Quantitative Measurement of the Iron‐Incorporating Enzyme in Relation to Marrow Cells and Liver Tissue in the Rabbit

Lochhead, Ann C.; Kramer, S. D.; Goldberg, A.

doi:10.1111/j.1365-2141.1963.tb05439.x

Cited by 15 publications

(5 citation statements)

References 22 publications

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“…The mean concentrations of lead in the unhomogenized lead-poisoned tissues were of a similar order to the concentrations which produced inhibition when added to normal tissue homogenates ( 1 0 -4~ and 1 0 -3~) , but the process of homogenization and preparation of the final incubation medium had reduced these mean concentrations twenty-fold, while the substrates iron and protoporphyrin were added in excess. Lochhead, Kramer & Goldberg (1963) found that ascorbic acid (5 pmole) and glutathione (75 pmole) in an incubation mixture of 10 ml markedly increased the activity of haem synthetase in rabbit bone marrow cells. In contrast the bone marrow in the present experiments showed little capacity for stimulation by reducing agents, or anaerobic conditions.…”

Section: Haem Synthetase Activitymentioning

confidence: 98%

Defects in Haem Synthesis in Mammalian Tissues in Experimental Lead Poisoning and Experimental Porphyria

Gibson

Goldberg

1970

Clinical Science

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1. The biochemical effects of experimental lead poisoning and experimental porphyria induced by allyl-isopropylacetamide have been compared in New Zealand White rabbits. 2. In lead poisoning there was an increase in urinary coproporphyrin excretion within 48 hr of the first dose of lead. Urinary δ-aminolaevulic acid excretion increased about 1 week later and urinary porphobilinogen excretion about 3 weeks later. 3. In lead-poisoned rabbits the activity of the enzyme δ-aminolaevulic acid dehydrase was impaired in the brain, liver, kidney and bone marrow. This inhibition was largely due to interference with the sulphydryl groups of the enzyme. 4. No inhibition of the enzyme haem synthetase could be detected in homogenates prepared from lead poisoned tissues but the addition of lead acetate in concentrations similar to those present in the original unhomogenized tissues produced marked inhibition. 5. There was no increase in either the δ-aminolaevulic acid or the porphobilinogen content of the brain in lead poisoning but the δ-aminolaevulic acid content of the liver, kidney and bone marrow, and the porphobiliogen content of the kidney were significantly increased. 6. Inhibition by lead of the enzymes δ-aminolaevulic acid dehydrase and haem synthetase may explain the biochemical abnormalities observed in lead poisoning. 7. In experimental porphyria the main enzyme abnormality found was a large increase in δ-aminolaevulic acid synthetase activity in the liver with increases in δ-aminolaevulic acid dehydrase activity in the liver and kidney.

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Section: Haem Synthetase Activitymentioning

confidence: 98%

Defects in Haem Synthesis in Mammalian Tissues in Experimental Lead Poisoning and Experimental Porphyria

Gibson

Goldberg

1970

Clinical Science

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“…Accumulation of iron within the normoblasts in the presence of hypochromic cells suggests a block in the utilization of iron for haem synthesis, and as a first step in evaluation of this, we have nieasured thc enzymatic incorporation, by reticulocytes, of iron into protoporphyrin 82 M. S. Losowsky and R. Hal! using the technique described by Lochhead, Kramer and Goldberg (1963). Results SO far show no diminution of iron incorporation in patients as compared with controls, and studics of the earlier stages of haem synthesis are now in progress.…”

Section: Further Studiesmentioning

confidence: 89%

Hereditary Sideroblastic Anaemia

Losowsky

Hall

1965

Br J Haematol

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CASES of sideroblastic anaemia without an obvious underlying cause and with other members of the family affected by what is clearly the same condition are few in the literature, and in most reports the family study has not been sufficiently extensive to establish the type of inheritance or even to ensure that the cause is hereditary rather than environmental. TABLE I a) SIDEROBLASTIC AND FAMILIAL Reference Sex-linked recessive inheritance Lukl et a/. (1958) Genetics uncertain Dacie (1954) Heilmeyer ef al. (1958) Byrd and Cooper (1961) Verloop et a/. (1962) Redmond et al. (1963) Notes Large family Mother and children z brothers a. 2 brothers b. 2 brothers, ? others a. z males, I female b. z males ( I P.R.A.) c. I male a. z. brothers (I P.R.A.) b. 2 brothers, ? others (b) SIDEROBLASTIC AND ? FAMILIAL Notes I Reference Mills et a/. (1950) Caroli ef al. (1957) Garby et a/. (1957) Verloop and Rademaker (1960) Male+? father, daughter Male (P.R.A.) + ? uncle Male+ ? sister, others Male (P.R.A.) + ? 3 children ~ ~~~ In this and subsequent tables, P.R.A. indicates a case of pyridoxineresponsive anaemia. Reference Sex-linked recessive inheritance Cooley (1945) Rundles and Falls (1946) Bourne et al. (1965) Mills and Lucia (1949) Gelpi and Ende (1958) Liidin (1957) Medal et al. (1961) Cotton and Harris (1962) Genetics uncertain Notes Large family a. Large family b. Large family (I P.R.A.) (Bishop and Bethell) Large family (3 P.R.A.) z half-brothers, son z brothers, ? others a. Father, 2 sons b. Brother and sister, her son z brothers, ? sister (2 P.R.A.)

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“…This work has been recently extended to mammalian erythroid tissue by Lochhead, Kramer & Goldberg (1963) who demonstrated activity of the enzyme in rabbit bone marrow. Vavra, Mayer & Moore (1964) reported low activities in human peripheral blood from a variety of patients.…”

Section: The Influence Of 24-dichlorophenoxyaceticmentioning

confidence: 98%

Proceedings of The Biochemical Society

1966

Biochemical Journal

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The discovery and production of selective herbicides in the past decade has been phenomenal. Even so many weed-control problems still remain unsolved and there is a continuing demand for new herbicides active against particular weeds and characterized by improved selectivity. A historical review of our most effective pesticides reveals that the discovery of biological activity in synthetic chemicals has, in most instances, resulted from empiricism or chance (Sexton, 1963; Moorefield, 1965). In the future, however, we shall rely more and more on an improved knowledge of the physiology and biochemistry of herbicide interactions to modify and extend the processes of empirical screening. To evolve a successful herbicide requires firstly the discovery, usually by chance, of a 'lead', i.e. a novel chemical structure possessing some degree of biological activity, and secondly a development programme wherein the relationships between chemical structure, biological activity and species selectivity are examined in an empirical screening programme (whole plants). In the progression of

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Quantitative Measurement of the Iron‐Incorporating Enzyme in Relation to Marrow Cells and Liver Tissue in the Rabbit

Cited by 15 publications

References 22 publications

Defects in Haem Synthesis in Mammalian Tissues in Experimental Lead Poisoning and Experimental Porphyria

Defects in Haem Synthesis in Mammalian Tissues in Experimental Lead Poisoning and Experimental Porphyria

Hereditary Sideroblastic Anaemia

Proceedings of The Biochemical Society

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