Quantitative measurements of absorption spectra in scattering media by low-coherence spectroscopy

Bosschaart, Nienke; Aalders, Maurice C. G.; Faber, Dirk J.; Weda, Jelmer J. A.; Gemert, Martin J. C. van; Leeuwen, Ton G. van

doi:10.1364/ol.34.003746

Cited by 31 publications

(43 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To compare the performance of the STFT to the DW method, we define a medium STFT window w M with Δz M = 22 µm in the spatial domain -as we used in our previous work [6][7][8] to quantify hemoglobin absorption in human skin -and a short (w S ) and a long (w L ) window for the DW method -comparable to those used by Robles et al [5] to quantify hemoglobin absorption in an animal model. Table 1 summarizes the window sizes in all domains and the resulting wavelength resolutions (Eq.…”

Section: Simulationsmentioning

confidence: 99%

“…Although the wavelength resolutions differ between the DW and STFT method (Table 1), N is equal for both methods in the 475-650 nm range, due to zeropadding in the calculation of the spectral/spatial distributions. Equation (6) shows that the lower χ 2 , the better the recovery of µ a .…”

Section: Quantification Of Performancementioning

confidence: 99%

“…sOCT and LCS not only enhance the contrast of OCT images [1][2][3][4][5], but also allow for the quantification of depth-and wavelength resolved optical properties, such as the absorption [5][6][7], scattering and backscattering coefficient [8,9]. These optical property spectra provide valuable information on the physiological status of tissue -for instance on the spatial distribution of hemoglobin concentration and oxygen saturation [7] and the identification of lipid in atherosclerotic plaques [10].…”

Section: Introductionmentioning

confidence: 99%

“…Methods that have been applied in practice for the extraction of spectral information from LCI signals are the short-time Fourier transform (STFT) [4,[6][7][8][9][10], wavelet transforms [3], the Wigner-Ville distribution (WV) [11] and the dual window method (DW) [5,12]. In general, all methods aim to recover spectra from distinct depth intervals in the LCI data set.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Quantitative comparison of analysis methods for spectroscopic optical coherence tomography

Bosschaart

Leeuwen

Aalders

et al. 2013

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Spectroscopic optical coherence tomography (sOCT) enables the mapping of chromophore concentrations and image contrast enhancement in tissue. Acquisition of depth resolved spectra by sOCT requires analysis methods with optimal spectral/spatial resolution and spectral recovery. In this article, we quantitatively compare the available methods, i.e. the short time Fourier transform (STFT), wavelet transforms, the Wigner-Ville distribution and the dual window method through simulations in tissue-like media. We conclude that all methods suffer from the trade-off in spectral/spatial resolution, and that the STFT is the optimal method for the specific application of the localized quantification of hemoglobin concentration and oxygen saturation.

show abstract

Section: Simulationsmentioning

confidence: 99%

Section: Quantification Of Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Quantitative comparison of analysis methods for spectroscopic optical coherence tomography

Bosschaart

Leeuwen

Aalders

et al. 2013

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…Advances in OCT have allowed for acquisition of depth-resolved functional information using SOCT [6][7][8], introducing the possibility to improve current clinical methods for quantifying tissue properties in vivo [9,10]. Compared to the other optical techniques mentioned above, SOCT provides spectroscopic information with high spatial resolution allowing for functional information to be directly overlaid with images of tissue structure.…”

Section: Introductionmentioning

confidence: 99%

Assessing hemoglobin concentration using spectroscopic optical coherence tomography for feasibility of tissue diagnostics

Robles¹,

Chowdhury²,

Wax³

2010

Biomed. Opt. Express

120

View full text Add to dashboard Cite

Hemoglobin (Hb) concentration and oxygen saturation levels are important biomarkers for various diseases, including cancer. Here, we investigate the ability to measure these parameters for tissue using spectroscopic optical coherence tomography (SOCT). A parallel frequency domain OCT system is used with detection spanning the visible region of the spectrum (450 nm to 700 nm). Oxygenated and deoxygenated Hb absorbing phantoms are analyzed. The results show that Hb concentrations as low as 1.2 g/L at 1 mm can be retrieved indicating that both normal and cancerous tissue measurements may be obtained. However, measurement of oxygen saturation levels may not be achieved with this approach.

show abstract

Towards non‐invasive tissue hydration measurements with optical coherence tomography

Neubrand,

van Leeuwen,

Faber

2024

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

The attenuation coefficient () measured by optical coherence tomography (OCT) has been used to determine tissue hydration. Previous dual‐wavelength OCT systems could not attain the needed precision, which we attribute to the absence of wavelength‐dependent scattering of tissue in the underlying model. Assuming that scattering can be described using two parameters, we propose a triple/quadrupole‐OCT system to achieve clinically relevant precision in water volume fraction. In this study, we conduct a quantitative analysis to determine the necessary precision of measurements and compare it with numerical simulation. Our findings emphasize that achieving a clinically relevant assessment of a 2% water fraction requires determining the attenuation coefficient with a remarkable precision of 0.01 . This precision threshold is influenced by the chosen wavelength for attenuation measurement and can be enhanced through the inclusion of a fourth wavelength range.

show abstract

Quantitative measurements of absorption spectra in scattering media by low-coherence spectroscopy

Cited by 31 publications

References 13 publications

Quantitative comparison of analysis methods for spectroscopic optical coherence tomography

Quantitative comparison of analysis methods for spectroscopic optical coherence tomography

Assessing hemoglobin concentration using spectroscopic optical coherence tomography for feasibility of tissue diagnostics

Towards non‐invasive tissue hydration measurements with optical coherence tomography

Contact Info

Product

Resources

About