Quantitative measurements of zebrafish heartrate and heart rate variability: A survey between 1990–2020

Ling, Dongmin; Chen, Huanxian; Chan, Ging; Lee, Simon Ming‐Yuen

doi:10.1016/j.compbiomed.2021.105045

Cited by 15 publications

(9 citation statements)

References 94 publications

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“…To analyze the ventilatory response and cardiac activity, the ventilatory frequency and heart rate, as well as the ventilatory interval and heartbeat coefficients of variation, based on heart rate variability analysis 19 , 20 , 45 , were calculated from the measured ventilatory signals and ECG. The measured data were then fitted using a linear mixed model for statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

“…However, the autonomic nervous system 10,11 and gill ventilation [12][13][14] are not fully functional in the larval stage; hence, the pharmacological effects exerted on these functions cannot be assessed until later stages.Zebrafish are commonly used as a human heart disease model [15][16][17][18] . In fact, cardiac activity is the most common physiological index assessed in adult zebrafish 19,20 . The heart rate of adult zebrafish is influenced by autonomic nervous system activity, and the cardiac action potential is similar to that of humans.…”

mentioning

confidence: 99%

“…The heart rate of adult zebrafish is influenced by autonomic nervous system activity, and the cardiac action potential is similar to that of humans. As such, the autonomic activity of the zebrafish heart is often assessed by measuring heart rate, and the associated variability, as in humans 19 . However, given that cardiac potential measurements require insertion of needle electrodes, spontaneous cardiac responses are not readily captured due to stress from restraint and nociception.Ventilation is also an important physiological index and is closely related to the emotion-like state of fish.…”

mentioning

confidence: 99%

“…Zebrafish are commonly used as a human heart disease model [15][16][17][18] . In fact, cardiac activity is the most common physiological index assessed in adult zebrafish 19,20 . The heart rate of adult zebrafish is influenced by autonomic nervous system activity, and the cardiac action potential is similar to that of humans.…”

mentioning

confidence: 99%

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Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions

Harada

Soh

Wakitani

et al. 2022

Sci Rep

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The zebrafish is widely used as a model in biological studies. In particular, the heart rate and cortisol levels of zebrafish are commonly measured to elucidate the pharmacological effects of chemical substances. Meanwhile, although ventilation is also an important physiological index reflecting emotion-like states, few studies have evaluated the effects of chemicals on ventilation in adult zebrafish. In this study, we assessed whether it is possible to evaluate the pharmacological effects elicited by caffeine in adult zebrafish under free-swimming conditions. We measured the ventilation in adult zebrafish exposed to multiple concentrations of caffeine under restraint and free-swimming conditions and evaluated the pharmacological effects of caffeine using linear mixed model analysis. In addition, results of electrocardiogram analysis and swimming speeds were compared with those in previous reports to ensure that an appropriate dose of caffeine was administered. Under restraint conditions, caffeine significantly decreased heart rate and increased ventilation in a concentration-dependent manner. Under free-swimming conditions, the ventilation rate significantly increased with increasing caffeine concentration. These results indicate that the pharmacological effects elicited by chemicals on ventilation can be evaluated in free-swimming zebrafish.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions

Harada

Soh

Wakitani

et al. 2022

Sci Rep

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“…Nowadays, D. rerio is used for modeling cardiovascular pathologies due to the presence of human disease genes in the genome (Howe et al, 2013). Transparent zebrafish embryos and larvae are the most convenient for heart functioning registration and vessel map reconstruction with optical approaches (Ling et al, 2022). The zebrafish's heart and vascular anatomy are similar to that of other vertebrates, including humans (Isogai et al, 2001;Liu et al, 2016;Bowley et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Influence of hypomagnetic field on the heartbeat in zebrafish embryos

Крылов

Мачихин²,

Sizov³

et al. 2022

Front. Physiol.

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The magnetic environment may influence the functioning of the cardiovascular system. It was reported that low-frequency and static magnetic fields affect hemodynamics, heart rate, and heart rate variability in animals and humans. Moreover, recent data suggest that magnetic fields affect the circadian rhythms of physiological processes. The influence of the magnetic environment on heart functionating during early development has been studied insufficiently. We utilized transparent zebrafish embryos to evaluate the effect of the hypomagnetic field on the characteristics of cardiac function using a noninvasive optical approach based on photoplethysmographic microscopic imaging. The embryos were exposed to the geomagnetic and hypomagnetic fields from the second to the 116th hour post fertilization under a 16 h light/8 h dark cycle or constant illumination. The exposure of embryos to the hypomagnetic field in both lighting modes led to increased embryo mortality, the appearance of abnormal phenotypes, and a significant increase in the embryo’s heartbeat rate. The difference between maximal and minimal heartbeat intervals, maximal to minimal heartbeat intervals ratio, and the coefficient of variation of heartbeat rate were increased in the embryos exposed to the hypomagnetic field under constant illumination from 96 to 116 h post fertilization. The dynamics of heartbeat rate changes followed a circadian pattern in all studied groups except zebrafish exposed to the hypomagnetic field under constant illumination. The results demonstrate the importance of natural magnetic background for the early development of zebrafish. The possible mechanisms of observed effects are discussed.

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A novel automated method for the simultaneous detection of breathing frequency and amplitude in zebrafish (Danio rerio) embryos and larvae

et al. 2023

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Quantitative measurements of zebrafish heartrate and heart rate variability: A survey between 1990–2020

Cited by 15 publications

References 94 publications

Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions

Pharmacological effects of caffeine on ventilation in adult zebrafish under free-swimming conditions

Influence of hypomagnetic field on the heartbeat in zebrafish embryos

A novel automated method for the simultaneous detection of breathing frequency and amplitude in zebrafish (Danio rerio) embryos and larvae

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